This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miksicek, R. J. Articles by Leygue, E. Search for Related Content PubMed PubMed Citation Articles by Miksicek, R. J. Articles by Leygue, E.

Identification of a Novel Breast- and Salivary Gland-specific, Mucin-like Gene Strongly Expressed in Normal and Tumor Human Mammary Epithelium¹

Department of Physiology, Michigan State University, East Lansing, Michigan 48824 [R. J. M.]; Department of Pathology, University of Manitoba, Winnipeg, Manitoba, R3E 0W3 Canada [Y. M., P. H. W.]; Department of Biochemistry and Medical Genetics and Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, R3E 0W3 Canada [P. H. W., C. W., L. C. M., E. L.]

Expression profiling using the public expressed sequence tag (EST) and serial analysis of geneexpression (SAGE) databases resulted in the identification of a putativebreast-specific mRNA that we have termed small breast epithelial mucin (SBEM). Hybridization analysis performed on 43 normal human tissues revealed that the SBEM gene was only expressed in mammary and salivary glands. Further reverse-transcription PCR analyses confirmed SBEM expression in most of established human breast epithelial cell lines analyzed (7 of 8) but not in cell lines of non-breast origin (0 of 6). SBEM mRNA expression was detected in >90% of invasive ductal carcinomas and correlated with the expression of a previously characterized breast-specific gene, mammaglobin-1 (n = 54; Spearman r = 0.34, P = 0.011). Interestingly, a higher SBEM:mammaglobin-1 ratio was observed in primary tumors with axillary lymph node metastasis than in node-negative tumors (n = 46; Mann-Whitney, P = 0.04). In a subset of 20 primary breast tumors and their matched axillary lymph nodes, a high concordance (Fisher’s exact test, P < 0.001) was seen between PCR detection of SBEM mRNA in lymph node tissue and their histopathological status, indicating that SBEM mRNA expression is conserved in nodal metastasis. The SBEM gene is predicted to code for a putative low molecular weight, secreted sialoglycoprotein, potentially useful for the diagnosis of metastatic breast cancer.

This article has been cited by other articles:

				M. Lacroix Significance, detection and markers of disseminated breast cancer cells Endocr. Relat. Cancer, December 1, 2006; 13(4): 1033 - 1067. [Abstract] [Full Text] [PDF]

				F. Hube, Y. Myal, E. Leygue, J. Rollin, Y. Gruel, Y. Yatabe, and on behalf of the authors Expression of Two Breast-Specific Molecules in the Lung J. Mol. Diagn., July 1, 2006; 8(3): 390 - 393. [Full Text] [PDF]

				L. Radvanyi, D. Singh-Sandhu, S. Gallichan, C. Lovitt, A. Pedyczak, G. Mallo, K. Gish, K. Kwok, W. Hanna, J. Zubovits, et al. The gene associated with trichorhinophalangeal syndrome in humans is overexpressed in breast cancer PNAS, August 2, 2005; 102(31): 11005 - 11010. [Abstract] [Full Text] [PDF]

				L C Murphy, B Peng, A Lewis, J R Davie, E Leygue, A Kemp, K Ung, M Vendetti, and R Shiu Inducible upregulation of oestrogen receptor-{beta}1 affects oestrogen and tamoxifen responsiveness in MCF7 human breast cancer cells J. Mol. Endocrinol., April 1, 2005; 34(2): 553 - 566. [Abstract] [Full Text] [PDF]

				M. Mitas, J. S. Almeida, K. Mikhitarian, W. E. Gillanders, D. N. Lewin, D. D. Spyropoulos, L. Hoover, A. Graham, T. Glenn, P. King, et al. Accurate Discrimination of Barrett's Esophagus and Esophageal Adenocarcinoma Using a Quantitative Three-Tiered Algorithm and Multimarker Real-time Reverse Transcription-PCR Clin. Cancer Res., March 15, 2005; 11(6): 2205 - 2214. [Abstract] [Full Text] [PDF]

				T. Koga, Y. Horio, T. Mitsudomi, T. Takahashi, and Y. Yatabe Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available SAGE Data J. Mol. Diagn., May 1, 2004; 6(2): 90 - 95. [Abstract] [Full Text] [PDF]