This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yuan, J. Articles by Strebhardt, K. Search for Related Content PubMed PubMed Citation Articles by Yuan, J. Articles by Strebhardt, K.

Efficient Internalization of the Polo-Box of Polo-like Kinase 1 Fused to an Antennapedia Peptide Results in Inhibition of Cancer Cell Proliferation¹

Department of Obstetrics and Gynecology, Medical School, J.W. Goethe-University, 60590 Frankfurt, Germany

Polo-like kinases (Plk) regulate multiple stages in mitosis. Plk1 is overexpressed in tumors.The COOH-terminal regions of Plks contain a conserved domain, termed polo-box, which is required for subcellular localization and for physical interaction with substrates. We linked the polo-box (amino acids 410–429) of Plk1 to an Antennapedia peptide and studied its impact on tumor cells. Whereas the wild-type polo-box inhibited the proliferation of tumor cells associated with induction of apoptosis, a mutated derivative was much less effective. The treatment caused mitotic arrest, misaligned chromosomes, and multiple centrosomes. Taken together, membrane-permeable polo-box peptides inhibit cancer cell proliferation efficiently.

This article has been cited by other articles:

				N. Jiang, X. Wang, M. Jhanwar-Uniyal, Z. Darzynkiewicz, and W. Dai Polo Box Domain of Plk3 Functions as a Centrosome Localization Signal, Overexpression of Which Causes Mitotic Arrest, Cytokinesis Defects, and Apoptosis J. Biol. Chem., April 14, 2006; 281(15): 10577 - 10582. [Abstract] [Full Text] [PDF]

				F. Eckerdt, J. Yuan, K. Saxena, B. Martin, S. Kappel, C. Lindenau, A. Kramer, S. Naumann, S. Daum, G. Fischer, et al. Polo-like Kinase 1-mediated Phosphorylation Stabilizes Pin1 by Inhibiting Its Ubiquitination in Human Cells J. Biol. Chem., November 4, 2005; 280(44): 36575 - 36583. [Abstract] [Full Text] [PDF]

				R. Guan, P. Tapang, J. D. Leverson, D. Albert, V. L. Giranda, and Y. Luo Small Interfering RNA-Mediated Polo-Like Kinase 1 Depletion Preferentially Reduces the Survival of p53-Defective, Oncogenic Transformed Cells and Inhibits Tumor Growth in Animals Cancer Res., April 1, 2005; 65(7): 2698 - 2704. [Abstract] [Full Text] [PDF]

				L. Tsvetkov and D. F. Stern Interaction of Chromatin-associated Plk1 and Mcm7 J. Biol. Chem., March 25, 2005; 280(12): 11943 - 11947. [Abstract] [Full Text] [PDF]

				T. F. Burns, P. Fei, K. A. Scata, D. T. Dicker, and W. S. El-Deiry Silencing of the Novel p53 Target Gene Snk/Plk2 Leads to Mitotic Catastrophe in Paclitaxel (Taxol)-Exposed Cells Mol. Cell. Biol., August 15, 2003; 23(16): 5556 - 5571. [Abstract] [Full Text] [PDF]

				R. N. Re Intracellular Renin and the Nature of Intracrine Enzymes Hypertension, August 1, 2003; 42(2): 117 - 122. [Abstract] [Full Text] [PDF]

				B. Yang, L. Gao, L. Li, Z. Lu, X. Fan, C. A. Patel, R. J. Pomerantz, G. C. DuBois, and H. Zhang Potent Suppression of Viral Infectivity by the Peptides That Inhibit Multimerization of Human Immunodeficiency Virus Type 1 (HIV-1) Vif Proteins J. Biol. Chem., February 14, 2003; 278(8): 6596 - 6602. [Abstract] [Full Text] [PDF]