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Peptidomics-based Approach Reveals the Secretion of the 29-Residue COOH-Terminal Fragment of the Putative Tumor Suppressor Protein DMBT1 from Pancreatic Adenocarcinoma Cell Lines¹

Growth Factor Division [K. Sas., K. Sat., Y. A., K. Yam.] and Central Animal Laboratory [K. Yan.], National Cancer Center Research Institute, Tokyo 104-0045, and Department of Surgery II, Yamaguchi University School of Medicine, Yamaguchi 755-8505 [M. O.], Japan

Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung, esophageal,gastric, and colorectal cancer. Here we report the mass spectrometric identification of a 3335 Da peptide, which was found in serum-free conditioned medium from 5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2 nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated inside the cells by limited proteolysis and extracellularly secreted. Our peptidomics-based approach will help screen candidate marker peptides for a particular type of cancer.

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