Cancer Research Cancer Epigenetics Protein Translation and Cancer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, C.
Right arrow Articles by Stoner, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, C.
Right arrow Articles by Stoner, G. D.
[Cancer Research 62, 6857-6863, December 1, 2002]
© 2002 American Association for Cancer Research

Epidemiology and Prevention

Inhibition of Benzo(a)pyrene Diol-Epoxide-induced Transactivation of Activated Protein 1 and Nuclear Factor {kappa}B by Black Raspberry Extracts1

Chuanshu Huang2, Yi Huang3, Jingxia Li, Wenwei Hu, Robeena Aziz, Moon-shong Tang, Nanjun Sun, John Cassady and Gary D. Stoner

Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, New York 10987 [C. H., Y. H., J. L., W. H., M-s. T.], and Division of Environmental Health Sciences, School of Public Health [R. A., G. D. S.] and College of Pharmacy [N. S., J. C.], The Ohio State University, Columbus, Ohio 43210

Freeze-dried black raspberries have been shown to inhibit the development of chemically induced esophageal and colon cancer in rodents.In addition, organic extracts of black raspberries inhibit benzo(a)pyrene (BaP)-induced cell transformation in vitro. The molecular mechanisms through which black raspberries inhibit carcinogenesis remain unclear. We investigated the effects of black raspberry extracts on transactivation of activated protein 1 (AP-1) and nuclear factor {kappa}B (NF{kappa}B) induced by BaP diol-epoxide (BPDE), the ultimate carcinogen of BaP, in mouse epidermal JB6 Cl 41 (Cl 41) cells. Black raspberries were extracted with methanol, and the methanol extract was partitioned and chromatographed into several fractions designated RU-F003, RU-F004, RU-DM, and RU-ME. Pretreatment of Cl 41 cells with RU-F003, RU-DM, or RU-ME resulted in an inhibition of BPDE-induced AP-1 and NF{kappa}B activities. The RU-ME fraction was the most potent inhibitor among the fractions tested. In contrast, fraction RU-F004 did not inhibit BPDE-induced AP-1 or NF{kappa}B activities in Cl 41 cells. The inhibitory effects of RU-ME on BPDE-induced activation of AP-1 and NF{kappa}B appear to be mediated via inhibition of mitogen activated protein kinase activation and inhibitory subunit {kappa}B phosphorylation, respectively. Pretreatment of cells with berry fractions did not result in an inhibition of BPDE binding to DNA; thus, this was not a mechanism of reduced AP-1 and NF{kappa}B activities. None of the fractions was found to affect p53-dependent transcription activity. In view of the important roles of AP-1 and NF{kappa}B in tumor promotion/progression, these results suggest that the ability of black raspberries to inhibit tumor development may be mediated by impairing signal transduction pathways leading to activation of AP-1 and NF{kappa}B. The RU-ME fraction appears to be the major fraction responsible for the inhibitory activity of black raspberries.




This article has been cited by other articles:


Home page
 Cancer Prevention ResearchHome page
T. E. Johnson, F. Kassie, M. G. O'Sullivan, M. Negia, T. E. Hanson, P. Upadhyaya, P. P. Ruvolo, S. S. Hecht, and C. Xing
Chemopreventive Effect of Kava on 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone plus Benzo[a]pyrene-Induced Lung Tumorigenesis in A/J Mice
Cancer Prevention Research, November 1, 2008; 1(6): 430 - 438.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
G. D. Stoner, L.-S. Wang, and B. C. Casto
Laboratory and clinical studies of cancer chemoprevention by antioxidants in berries
Carcinogenesis, September 1, 2008; 29(9): 1665 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. R. Mallery, J. C. Zwick, P. Pei, M. Tong, P. E. Larsen, B. S. Shumway, B. Lu, H. W. Fields, R. J. Mumper, and G. D. Stoner
Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
Cancer Res., June 15, 2008; 68(12): 4945 - 4957.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
B. S. Shumway, L. A. Kresty, P. E. Larsen, J. C. Zwick, B. Lu, H. W. Fields, R. J. Mumper, G. D. Stoner, and S. R. Mallery
Effects of a Topically Applied Bioadhesive Berry Gel on Loss of Heterozygosity Indices in Premalignant Oral Lesions
Clin. Cancer Res., April 15, 2008; 14(8): 2421 - 2430.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
D. Zhang, J. Li, L. Song, W. Ouyang, J. Gao, and C. Huang
A JNK1/AP-1-Dependent, COX-2 Induction Is Implicated in 12-O-Tetradecanoylphorbol-13-Acetate-Induced Cell Transformation through Regulating Cell Cycle Progression
Mol. Cancer Res., January 1, 2008; 6(1): 165 - 174.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
D. Zhang, J. Li, K. Wu, W. Ouyang, J. Ding, Z.-g. Liu, M. Costa, and C. Huang
JNK1, but not JNK2, is required for COX-2 induction by nickel compounds
Carcinogenesis, April 1, 2007; 28(4): 883 - 891.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. W. Chung, H. Y. Chung, A. Toriba, T. Kameda, N. Tang, R. Kizu, and K. Hayakawa
An Environmental Quinoid Polycyclic Aromatic Hydrocarbon, Acenaphthenequinone, Modulates Cyclooxygenase-2 Expression through Reactive Oxygen Species Generation and Nuclear Factor Kappa B Activation in A549 Cells
Toxicol. Sci., February 1, 2007; 95(2): 348 - 355.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
T. Chen, M. E. Rose, H. Hwang, R. G. Nines, and G. D. Stoner
Black raspberries inhibit N-nitrosomethylbenzylamine (NMBA)-induced angiogenesis in rat esophagus parallel to the suppression of COX-2 and iNOS
Carcinogenesis, November 1, 2006; 27(11): 2301 - 2307.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. S. Hecht, C. Huang, G. D. Stoner, J. Li, P. M.J. Kenney, S. J. Sturla, and S. G. Carmella
Identification of cyanidin glycosides as constituents of freeze-dried black raspberries which inhibit anti-benzo[a]pyrene-7,8-diol-9,10-epoxide induced NF{kappa}B and AP-1 activity
Carcinogenesis, August 1, 2006; 27(8): 1617 - 1626.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Vanamala, T. Leonardi, B. S. Patil, S. S. Taddeo, M. E. Murphy, L. M. Pike, R. S. Chapkin, J. R. Lupton, and N. D. Turner
Suppression of colon carcinogenesis by bioactive compounds in grapefruit
Carcinogenesis, June 1, 2006; 27(6): 1257 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
R. P. Singh, S. Dhanalakshmi, S. Mohan, C. Agarwal, and R. Agarwal
Silibinin inhibits UVB- and epidermal growth factor-induced mitogenic and cell survival signaling involving activator protein-1 and nuclear factor-{kappa}B in mouse epidermal JB6 cells
Mol. Cancer Ther., May 1, 2006; 5(5): 1145 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Huang, J. Li, L. Song, D. Zhang, Q. Tong, M. Ding, L. Bowman, R. Aziz, and G. D. Stoner
Black Raspberry Extracts Inhibit Benzo(a)Pyrene Diol-Epoxide-Induced Activator Protein 1 Activation and VEGF Transcription by Targeting the Phosphotidylinositol 3-Kinase/Akt Pathway
Cancer Res., January 1, 2006; 66(1): 581 - 587.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Wang, W. Ouyang, J. Li, L. Wei, Q. Ma, Z. Zhang, Q. Tong, J. He, and C. Huang
Loss of Tumor Suppressor p53 Decreases PTEN Expression and Enhances Signaling Pathways Leading to Activation of Activator Protein 1 and Nuclear Factor {kappa}B Induced by UV Radiation
Cancer Res., August 1, 2005; 65(15): 6601 - 6611.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhao, Z. He, N. Chen, Y.-Y. Cho, F. Zhu, C. Lu, W.-y. Ma, A. M. Bode, and Z. Dong
2-Arachidonoylglycerol Stimulates Activator Protein-1-dependent Transcriptional Activity and Enhances Epidermal Growth Factor-induced Cell Transformation in JB6 P+ Cells
J. Biol. Chem., July 22, 2005; 280(29): 26735 - 26742.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Chen, Y. Yan, J. Li, Q. Ma, G. D. Stoner, J. Ye, and C. Huang
Differential requirement of signal pathways for benzo[a]pyrene (B[a]P)-induced nitric oxide synthase (iNOS) in rat esophageal epithelial cells
Carcinogenesis, June 1, 2005; 26(6): 1035 - 1043.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Dhanalakshmi, C. Agarwal, R. P. Singh, and R. Agarwal
Silibinin Up-regulates DNA-Protein Kinase-dependent p53 Activation to Enhance UVB-induced Apoptosis in Mouse Epithelial JB6 Cells
J. Biol. Chem., May 27, 2005; 280(21): 20375 - 20383.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. L. Sabichi, M.-F. Demierre, E. T. Hawk, C. E. Lerman, and S. M. Lippman
Frontiers in Cancer Prevention Research
Cancer Res., September 15, 2003; 63(18): 5649 - 5655.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2002 by the American Association for Cancer Research.