This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, C.-L. Articles by Tam, P. K. H. Search for Related Content PubMed PubMed Citation Articles by Lin, C.-L. Articles by Tam, P. K. H.

Immunization with Epstein-Barr Virus (EBV) Peptide-pulsed Dendritic Cells Induces Functional CD8+ T-Cell Immunity and May Lead to Tumor Regression in Patients with EBV-positive Nasopharyngeal Carcinoma¹

Department of Surgery, Chang Gung Memorial Hospital, Kaohsiung, 883 Taiwan [C-L. L., W-F. L., T-H. L., C-L. C.]; Department of Surgery, The University of Hong Kong Medical Centre, Queen Mary Hospital, Pokfulam Road, Hong Kong, SAR, China [C-L. L., Y. R., P. K. H. T.]; Department of Surgery, Kaohsiung Medical University, Kaohsiung, 807 Taiwan [S-L. H.]; Department of Radiology, Chang Gung Memorial Hospital, Kaohsiung, 883 Taiwan [Y-F. C.]; Graduate Institute of Basic Medical Sciences, Chang Gung University, Tao-Yuan, 333 Taiwan [Y-S. C.]; and CRC Institute for Cancer Studies, Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT United Kingdom [S. P. L., A. B. R.]

Nasopharyngeal carcinoma (NPC), a common neoplasm in Southeast Asia, is EBV-positive and expresses a limited number of antigens, including latent membrane protein 2. In this study, autologous monocyte-derived dendritic cells were cultured from patients with advanced NPC, matured with cytokine, pulsed with HLA-A1101-, A2402-, or B40011-restricted epitope peptides from EBV latent membrane protein 2 and injected into inguinal lymph nodes. Sixteen patients with local recurrence or distant metastasis after conventional therapies received four injections at weekly intervals. Epitope-specific CD8+ T-cell responses were elicited or boosted in 9 patients receiving HLA-A1101- or A2402-restricted peptides, with stronger responses seen to the A1101 peptide. Furthermore, epitope-specific cytotoxicity was detectable in peripheral blood T cells harvested at 3-months after vaccination from A1101-responsive patients, and in 2 patients, this coincided with partial tumor reduction. Approaches leading to stronger and more sustained EBV-specific T-cell responses, therefore, may have therapeutic potential in the context of NPC.

This article has been cited by other articles:

				S. L. Elliott, A. Suhrbier, J. J. Miles, G. Lawrence, S. J. Pye, T. T. Le, A. Rosenstengel, T. Nguyen, A. Allworth, S. R. Burrows, et al. Phase I Trial of a CD8+ T-Cell Peptide Epitope-Based Vaccine for Infectious Mononucleosis J. Virol., February 1, 2008; 82(3): 1448 - 1457. [Abstract] [Full Text] [PDF]

				C. W. Tsang, X. Lin, N. H. Gudgeon, G. S. Taylor, H. Jia, E. P. Hui, A. T. C. Chan, C. K. Lin, and A. B. Rickinson CD4+ T-Cell Responses to Epstein-Barr Virus Nuclear Antigen EBNA1 in Chinese Populations Are Highly Focused on Novel C-Terminal Domain-Derived Epitopes. J. Virol., August 1, 2006; 80(16): 8263 - 8266. [Abstract] [Full Text] [PDF]

				K. N. Heller, C. Gurer, and C. Munz Virus-specific CD4+ T cells: ready for direct attack J. Exp. Med., April 17, 2006; 203(4): 805 - 808. [Abstract] [Full Text] [PDF]

				P. Comoli, P. Pedrazzoli, R. Maccario, S. Basso, O. Carminati, M. Labirio, R. Schiavo, S. Secondino, C. Frasson, C. Perotti, et al. Cell Therapy of Stage IV Nasopharyngeal Carcinoma With Autologous Epstein-Barr Virus-Targeted Cytotoxic T Lymphocytes J. Clin. Oncol., December 10, 2005; 23(35): 8942 - 8949. [Abstract] [Full Text] [PDF]

				K. C. Straathof, A. M. Leen, E. L. Buza, G. Taylor, M. H. Huls, H. E. Heslop, C. M. Rooney, and C. M. Bollard Characterization of Latent Membrane Protein 2 Specificity in CTL Lines from Patients with EBV-Positive Nasopharyngeal Carcinoma and Lymphoma J. Immunol., September 15, 2005; 175(6): 4137 - 4147. [Abstract] [Full Text] [PDF]

				K. C. M. Straathof, C. M. Bollard, U. Popat, M. H. Huls, T. Lopez, M. C. Morriss, M. V. Gresik, A. P. Gee, H. V. Russell, M. K. Brenner, et al. Treatment of nasopharyngeal carcinoma with Epstein-Barr virus-specific T lymphocytes Blood, March 1, 2005; 105(5): 1898 - 1904. [Abstract] [Full Text] [PDF]

				A. Nencioni and P. Brossart Cellular Immunotherapy with Dendritic Cells in Cancer: Current Status Stem Cells, July 1, 2004; 22(4): 501 - 513. [Abstract] [Full Text] [PDF]

				C. Munz Epstein-Barr Virus Nuclear Antigen 1: from Immunologically Invisible to a Promising T Cell Target J. Exp. Med., May 17, 2004; 199(10): 1301 - 1304. [Abstract] [Full Text] [PDF]

				G. S. Taylor, T. A. Haigh, N. H. Gudgeon, R. J. Phelps, S. P. Lee, N. M. Steven, and A. B. Rickinson Dual Stimulation of Epstein-Barr Virus (EBV)-Specific CD4+- and CD8+-T-Cell Responses by a Chimeric Antigen Construct: Potential Therapeutic Vaccine for EBV-Positive Nasopharyngeal Carcinoma J. Virol., January 15, 2004; 78(2): 768 - 778. [Abstract] [Full Text] [PDF]

				P. Comoli, R. De Palma, S. Siena, A. Nocera, S. Basso, F. Del Galdo, R. Schiavo, O. Carminati, A. Tagliamacco, G. F. Abbate, et al. Adoptive transfer of allogeneic Epstein-Barr virus (EBV)-specific cytotoxic T cells with in vitro antitumor activity boosts LMP2-specific immune response in a patient with EBV-related nasopharyngeal carcinoma Ann. Onc., January 1, 2004; 15(1): 113 - 117. [Abstract] [Full Text] [PDF]

				K. Brown, W. Gao, S. Alber, A. Trichel, M. Murphey-Corb, S. C. Watkins, A. Gambotto, and S. M. Barratt-Boyes Adenovirus-Transduced Dendritic Cells Injected into Skin or Lymph Node Prime Potent Simian Immunodeficiency Virus-Specific T Cell Immunity in Monkeys J. Immunol., December 15, 2003; 171(12): 6875 - 6882. [Abstract] [Full Text] [PDF]