| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Tumor Biology |
Departments of Radiation Oncology [J. H. A. M. K., K. I. E. M. W., A. S. E. L., L. A. M. P., J. B., A. J. v. d. K.], Otorhinolaryngology [K. I. E. M. W., H. A. M. M., F. J. A. v. d. H.], and Pathology and Maxillofacial Surgery [P. C. M. d. W.], University Medical Center Nijmegen, 6500 HB Nijmegen, The Netherlands, and Radiation Oncology and Toxicology, University of North Carolina, Chapel Hill, North Carolina 27599-7512 [J. A. R.]
Hypoxia is associated with tumor aggressiveness and is an important cause of resistance to radiation treatment. Assays of tumor hypoxia could provide selection tools for hypoxia-modifying treatments. This study correlated the exogenous 2-nitroimidazole hypoxia marker 1-[(2-hydroxy-3-piperidinyl)propyl]-2-nitroimidazole hydrochloride (pimonidazole) with the endogenous hypoxia-related marker carbonic anhydrase 9 (CA9) and with vascular parameters using immunohistochemical techniques and a computerized image analysis system. Tumor biopsies were obtained from patients with head and neck carcinomas that were potential candidates for a Phase II trial with accelerated radiotherapy combined with carbogen and nicotinamide (ARCON). If, after completion of the diagnostic workup, the eligibility criteria were met and informed consent was obtained, patients were treated with ARCON. Those patients that were not eligible or refused ARCON were treated with radiotherapy, surgery, or a combined modality. Forty-three biopsies were analyzed, and the results were related with treatment outcome. The distribution patterns of pimonidazole and CA9 were similar, although the CA9 signal was generally observed already at shorter distances from blood vessels. There was a weak but significant correlation between the relative tumor areas positive for pimonidazole binding and areas with CA9 expression. Locoregional tumor control was significantly lower for patients who had hypoxic tumors or tumors with low vascular density. The 2-year control rates were 48 versus 87% for tumors with high and low pimonidazole binding levels (stratified by median, P = 0.01) and 48 and 88% for tumors with low and high vascular density (stratified by median, P = 0.01). These associations disappeared in the subgroup of patients treated with ARCON. There was no relationship between the level of CA9 expression and treatment outcome. It is concluded that pimonidazole binding and vascular density can predict treatment outcome in head and neck cancer and may be useful as selection tools for hypoxia-modifying treatments. Pimonidazole and CA9 demonstrate concordant staining patterns, but the latter is a less specific marker for hypoxia.
This article has been cited by other articles:
|
|
 |
|
  G. Komar, M. Seppanen, O. Eskola, P. Lindholm, T. J. Gronroos, S. Forsback, H. Sipila, S. M. Evans, O. Solin, and H. Minn 18F-EF5: A New PET Tracer for Imaging Hypoxia in Head and Neck Cancer J. Nucl. Med., December 1, 2008; 49(12): 1944 - 1951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Riedl, P. Brader, P. B. Zanzonico, Y. S. Chun, Y. Woo, P. Singh, S. Carlin, B. Wen, C. C. Ling, H. Hricak, et al. Imaging Hypoxia in Orthotopic Rat Liver Tumors with Iodine 124-labeled Iodoazomycin Galactopyranoside PET Radiology, August 1, 2008; 248(2): 561 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chan, M. Koritzinsky, H. Zhao, R. Bindra, P. M. Glazer, S. Powell, A. Belmaaza, B. Wouters, and R. G. Bristow Chronic Hypoxia Decreases Synthesis of Homologous Recombination Proteins to Offset Chemoresistance and Radioresistance Cancer Res., January 15, 2008; 68(2): 605 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Kim, H. J. Shin, K.-Y. Jung, S.-K. Baek, B. K. Shin, J. Choi, B. S. Kim, S. W. Shin, Y. H. Kim, J. S. Kim, et al. Prognostic Value of Carbonic Anhydrase IX and Ki-67 Expression in Squamous Cell Carcinoma of the Tongue Jpn. J. Clin. Oncol., November 1, 2007; 37(11): 812 - 819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Overgaard Hypoxic Radiosensitization: Adored and Ignored J. Clin. Oncol., September 10, 2007; 25(26): 4066 - 4074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Riesterer, L. Milas, and K. K. Ang Use of Molecular Biomarkers for Predicting the Response to Radiotherapy With or Without Chemotherapy J. Clin. Oncol., September 10, 2007; 25(26): 4075 - 4083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-F. Li, S. Carlin, M. Urano, J. Russell, C. C. Ling, and J. A. O'Donoghue Visualization of Hypoxia in Microscopic Tumors by Immunofluorescent Microscopy Cancer Res., August 15, 2007; 67(16): 7646 - 7653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yoo, G. S. Baia, J. S. Smith, M. W. McDermott, A. W. Bollen, S. R. VandenBerg, K. R. Lamborn, and A. Lal Expression of the Hypoxia Marker Carbonic Anhydrase 9 Is Associated with Anaplastic Phenotypes in Meningiomas Clin. Cancer Res., January 1, 2007; 13(1): 68 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Patiar and A. L Harris Role of hypoxia-inducible factor-1{alpha} as a cancer therapy target Endocr. Relat. Cancer, December 1, 2006; 13(Supplement_1): S61 - S75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Y Isa, T H Ward, C M L West, N J Slevin, and J J Homer Hypoxia in head and neck cancer Br. J. Radiol., October 1, 2006; 79(946): 791 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P P Dendy and P Wardman Hypoxia in biology and medicine: the legacy of L H Gray. Br. J. Radiol., July 1, 2006; 79(943): 545 - 549. [Full Text] [PDF]
|
|
|
|
|
|
K Newbold, M Partridge, G Cook, S A Sohaib, E Charles-Edwards, P Rhys-Evans, K Harrington, and C Nutting Advanced imaging applied to radiotherapy planning in head and neck cancer: a clinical review. Br. J. Radiol., July 1, 2006; 79(943): 554 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rischin, R. J. Hicks, R. Fisher, D. Binns, J. Corry, S. Porceddu, and L. J. Peters Prognostic Significance of [18F]-Misonidazole Positron Emission Tomography-Detected Tumor Hypoxia in Patients With Advanced Head and Neck Cancer Randomly Assigned to Chemoradiation With or Without Tirapazamine: A Substudy of Trans-Tasman Radiation Oncology Group Study 98.02 J. Clin. Oncol., May 1, 2006; 24(13): 2098 - 2104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-c. Zhang, S. Hironaka, A. Ohtsu, S. Yoshida, T. Hasebe, M. Fukayama, and A. Ochiai Computer-assisted analysis of biopsy specimen microvessels predicts the outcome of esophageal cancers treated with chemoradiotherapy. Clin. Cancer Res., March 15, 2006; 12(6): 1735 - 1742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jokilehto, K. Rantanen, M. Luukkaa, P. Heikkinen, R. Grenman, H. Minn, P. Kronqvist, and P. M. Jaakkola Overexpression and Nuclear Translocation of Hypoxia-Inducible Factor Prolyl Hydroxylase PHD2 in Head and Neck Squamous Cell Carcinoma Is Associated with Tumor Aggressiveness Clin. Cancer Res., February 15, 2006; 12(4): 1080 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Koukourakis, S. M. Bentzen, A. Giatromanolaki, G. D. Wilson, F. M. Daley, M. I. Saunders, S. Dische, E. Sivridis, and A. L. Harris Endogenous Markers of Two Separate Hypoxia Response Pathways (hypoxia inducible factor 2 alpha and carbonic anhydrase 9) Are Associated With Radiotherapy Failure in Head and Neck Cancer Patients Recruited in the CHART Randomized Trial J. Clin. Oncol., February 10, 2006; 24(5): 727 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mayer, M. Hockel, and P. Vaupel Carbonic Anhydrase IX Expression and Tumor Oxygenation Status Do Not Correlate at the Microregional Level in Locally Advanced Cancers of the Uterine Cervix Clin. Cancer Res., October 15, 2005; 11(20): 7220 - 7225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sobhanifar, C. Aquino-Parsons, E. J. Stanbridge, and P. Olive Reduced Expression of Hypoxia-Inducible Factor-1{alpha} in Perinecrotic Regions of Solid Tumors Cancer Res., August 15, 2005; 65(16): 7259 - 7266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. Arcasoy, K. Amin, S.-C. Chou, Z. A. Haroon, M. Varia, and J. A. Raleigh Erythropoietin and Erythropoietin Receptor Expression in Head and Neck Cancer: Relationship to Tumor Hypoxia Clin. Cancer Res., January 1, 2005; 11(1): 20 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Hoogsteen, H. A.M. Marres, K. I.E.M. Wijffels, P. F.J.W. Rijken, J. P.W. Peters, F. J.A. van den Hoogen, E. Oosterwijk, A. J. van der Kogel, and J. H.A.M. Kaanders Colocalization of Carbonic Anhydrase 9 Expression and Cell Proliferation in Human Head and Neck Squamous Cell Carcinoma Clin. Cancer Res., January 1, 2005; 11(1): 97 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hutchison, H. R. Valentine, J. A. Loncaster, S. E. Davidson, R. D. Hunter, S. A. Roberts, A. L. Harris, I. J. Stratford, P. M. Price, and C. M. L. West Hypoxia-Inducible Factor 1{alpha} Expression as an Intrinsic Marker of Hypoxia: Correlation with Tumor Oxygen, Pimonidazole Measurements, and Outcome in Locally Advanced Carcinoma of the Cervix Clin. Cancer Res., December 15, 2004; 10(24): 8405 - 8412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kim, Z. N. Rabbani, R. T. Vollmer, E.-G. Schreiber, E. Oosterwijk, M. W. Dewhirst, Z. Vujaskovic, and M. J. Kelley Carbonic Anhydrase IX in Early-Stage Non-Small Cell Lung Cancer Clin. Cancer Res., December 1, 2004; 10(23): 7925 - 7933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Maseide, R. A. Kandel, R. S. Bell, C. N. Catton, B. O'Sullivan, J. S. Wunder, M. Pintilie, D. Hedley, and R. P. Hill Carbonic Anhydrase IX as a Marker for Poor Prognosis in Soft Tissue Sarcoma Clin. Cancer Res., July 1, 2004; 10(13): 4464 - 4471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Cairns and R. P. Hill Acute Hypoxia Enhances Spontaneous Lymph Node Metastasis in an Orthotopic Murine Model of Human Cervical Carcinoma Cancer Res., March 15, 2004; 64(6): 2054 - 2061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Evans, K. D. Judy, I. Dunphy, W. T. Jenkins, P. T. Nelson, R. Collins, E. P. Wileyto, K. Jenkins, S. M. Hahn, C. W. Stevens, et al. Comparative Measurements of Hypoxia in Human Brain Tumors Using Needle Electrodes and EF5 Binding Cancer Res., March 1, 2004; 64(5): 1886 - 1892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-i. Kusakai, A. Suzuki, T. Ogura, S.'i. Miyamoto, A. Ochiai, M. Kaminishi, and H. Esumi ARK5 Expression in Colorectal Cancer and Its Implications for Tumor Progression Am. J. Pathol., March 1, 2004; 164(3): 987 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bassnett and R. McNulty The effect of elevated intraocular oxygen on organelle degradation in the embryonic chicken lens J. Exp. Biol., December 1, 2003; 206(23): 4353 - 4361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hedley, M. Pintilie, J. Woo, A. Morrison, D. Birle, A. Fyles, M. Milosevic, and R. Hill Carbonic Anhydrase IX Expression, Hypoxia, and Prognosis in Patients with Uterine Cervical Carcinomas Clin. Cancer Res., November 15, 2003; 9(15): 5666 - 5674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Azuma, S.-C. Chou, R. A. Lininger, B. J. Murphy, M. A. Varia, and J. A. Raleigh Hypoxia and Differentiation in Squamous Cell Carcinomas of the Uterine Cervix: Pimonidazole and Involucrin Clin. Cancer Res., October 15, 2003; 9(13): 4944 - 4952. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
