Cancer Research CTRC-AACR San Antonio Breast Cancer Symposium AACR Conference on Molecular Diagnostics - 2008
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[Cancer Research 62, 7166-7169, December 15, 2002]
© 2002 American Association for Cancer Research

Advances in Brief

GFP Expression in the Mammary Gland for Imaging of Mammary Tumor Cells in Transgenic Mice1

Fayyaz Ahmed, Jeffrey Wyckoff, Elaine Y. Lin, Weigang Wang, Yarong Wang, Lothar Hennighausen, Jun-ichi Miyazaki, Joan Jones, Jeffrey W. Pollard, John S. Condeelis and Jeffrey E. Segall2

Departments of Anatomy and Structural Biology [F. A., J. W., W. W., Y. W., J. S. C., J. E. S.], Developmental and Molecular Biology [E. Y. L., J. W. P.], and Pathology [J. J.], Albert Einstein College of Medicine, Bronx, New York 10461; Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 [L. H.]; and Division of Stem Cell Regulation Research, Osaka University Medical School, Osaka 565-0871, Japan [J. M.]

To examine the behavior of tumor cells in tumors developing directly from mammary tissue in transgenic models, we have evaluated transgenic mice expressing green fluorescent protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific. These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution.




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