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8-Hydroxyguanosine Repair Is Defective in Some Microsatellite Stable Colorectal Cancer Cells¹

Departments of Pathology [A. R. P., D. H. O., L. H., J. R. E.], Oncology [W. G. N., T. L. D., J. R. E.], Urology [R. N. O., W. G. N., T. L. D.], and Pharmacology and Medicine [W. G. N.], Johns Hopkins University, Baltimore, Maryland 21205

Mutator phenotypes are involved in the carcinogenesis of some cancers, e.g., defects in mismatch repair produce a mutator phenotype that drives carcinogenesis and causes microsatellite instability in hereditary nonpolyposis colon cancers and some sporadic colorectal cancers (CRC). Less understood, however, is the potential role of mutator phenotypes in microsatellite stable (MSS) CRC carcinogenesis. A novel transversion mutator phenotype was reported recently in an MSS CRC cell line. We hypothesized that 8-hydroxyguanosine could be involved and found elevations in 5 of 15 (33%) MSS CRC cell lines analyzed. Repair of an adenine8-hydroxyguanosine mispair was functionally defective in the same five cell lines. The human MutY homologue transcript and MutY homologue protein levels were also decreased. These findings may reflect a MSS mutator phenotype contributing to the development of CRC.

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