| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Carcinogenesis |
Molecular Epidemiology Laboratory, Toxicology and Molecular Biology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505
The molecular mechanisms potentially responsible for cell transformation and tumorigenesis induced by cadmium, a human carcinogen, were investigated by differential gene expression analysis of BALB/c-3T3 cells transformed with cadmium chloride (CdCl2). Differential display analysis of gene expression revealed consistent overexpression of mouse translation initiation factor 3 (TIF3; GenBank accession number AF271072) in the cells transformed with CdCl2 when compared with nontransformed cells. The predicted protein encoded by TIF3 cDNA exhibited 99% similarity to human eukaryotic initiation factor 3 p36 protein. A Mr 36,000 protein was detected in cells transfected with an expression vector containing TIF3 cDNA. Transfection of NIH3T3 cells with an expression vector containing TIF3 cDNA resulted in overexpression of the encoded protein, and this was associated with cell transformation, as evidenced by the appearance of transformed foci exhibiting anchorage-independent growth on soft agar and tumorigenic potential in nude mice. Expression of the antisense RNA against TIF3 mRNA resulted in significant reversal of oncogenic potential of the CdCl2-transformed BALB/c-3T3 cells. Taken together, these findings demonstrate for the first time that the cell transformation and tumorigenesis induced by CdCl2 are due, at least in part, to the overexpression of TIF3, a novel cadmium-responsive proto-oncogene.
This article has been cited by other articles:
|
|
 |
|
  L. Zhang, X. Pan, and J. W. B. Hershey Individual Overexpression of Five Subunits of Human Translation Initiation Factor eIF3 Promotes Malignant Transformation of Immortal Fibroblast Cells J. Biol. Chem., February 23, 2007; 282(8): 5790 - 5800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. An, J.-K. Chen, H.-j. Chen, W. Chang, Y.-G. Jiang, Q.-Y. Wei, and X.-M. Chen Characterization of 67 kD Laminin Receptor, a Protein Whose Gene Is Overexpressed on Treatment of Cells with Anti-Benzo[a]pyrene-7,8-Diol-9,10-Epoxide Toxicol. Sci., April 1, 2006; 90(2): 326 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Othumpangat, M. Kashon, and P. Joseph Eukaryotic Translation Initiation Factor 4E Is a Cellular Target for Toxicity and Death Due to Exposure to Cadmium Chloride J. Biol. Chem., July 1, 2005; 280(26): 25162 - 25169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Guan, S.-F. Jia, Z. Zhou, J. Stewart, and E. S. Kleinerman Herceptin Down-Regulates HER-2/neu and Vascular Endothelial Growth Factor Expression and Enhances Taxol-Induced Cytotoxicity of Human Ewing's Sarcoma Cells In vitro and In vivo Clin. Cancer Res., March 1, 2005; 11(5): 2008 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-K. Jeon, H.-S. Jin, D.-H. Lee, W.-S. Choi, C.-K. Moon, Y. J. Oh, and T. H. Lee Proteome Analysis Associated with Cadmium Adaptation in U937 Cells: IDENTIFICATION OF CALBINDIN-D28k AS A SECONDARY CADMIUM-RESPONSIVE PROTEIN THAT CONFERS RESISTANCE TO CADMIUM-INDUCED APOPTOSIS J. Biol. Chem., July 23, 2004; 279(30): 31575 - 31583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iida, C. H. Anna, J. Hartis, M. Bruno, B. Wetmore, J. R. Dubin, S. Sieber, L. Bennett, M. L. Cunningham, R. S. Paules, et al. Changes in global gene and protein expression during early mouse liver carcinogenesis induced by non-genotoxic model carcinogens oxazepam and Wyeth-14,643 Carcinogenesis, April 1, 2003; 24(4): 757 - 770. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Cell Growth & Differentiation |
