Cancer Research Donn Young EMT and Cancer Progression and Treatment
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bunz, F.
Right arrow Articles by Lengauer, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bunz, F.
Right arrow Articles by Lengauer, C.
[Cancer Research 62, 1129-1133, February 15, 2002]
© 2002 American Association for Cancer Research

Molecular Biology and Genetics

Targeted Inactivation of p53 in Human Cells Does Not Result in Aneuploidy1

Fred Bunz, Christine Fauth, Michael R. Speicher, Annie Dutriaux, John M. Sedivy, Kenneth W. Kinzler, Bert Vogelstein and Christoph Lengauer2

The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, [F. B., K. W. K., B. V., C. L.] and the Howard Hughes Medical Institute [B. V.], Johns Hopkins University, Baltimore, Maryland 21231; Institute of Anthropology and Human Genetics, University of Munich, Munich 80333, Germany [C. F., M. R. S.]; and Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912 [A. D., J. M. S.]

Because p53 mutation and aneuploidy usually coexist, it has been suggested that p53 inactivation leads to aneuploidy. We have rigorously tested this hypothesis in diploid human cell lines in which p53 was experimentally inactivated by targeted homologous recombination. Cells completely deficient in p53 did not become aneuploid, although a slight tendency toward tetraploidization was observed. No increased rates of numerical or structural chromosomal instabilities were observed in the p53-deficient cells. Rates of sister chromatid exchange and homologous recombination were also unaffected by p53 status. These results show that inactivation of p53 does not, in and of itself, lead to the development of aneuploidy.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. Ali, D. A. Heathcote, S. H.B. Kroll, A. S. Jogalekar, B. Scheiper, H. Patel, J. Brackow, A. Siwicka, M. J. Fuchter, M. Periyasamy, et al.
The Development of a Selective Cyclin-Dependent Kinase Inhibitor That Shows Antitumor Activity
Cancer Res., August 1, 2009; 69(15): 6208 - 6215.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. M. Torres, B. R. Williams, and A. Amon
Aneuploidy: Cells Losing Their Balance
Genetics, June 1, 2008; 179(2): 737 - 746.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Li, J. Tan, L. Zhuang, B. Banerjee, X. Yang, J. F. L. Chau, P. L. Lee, M. P. Hande, B. Li, and Q. Yu
Ribosomal Protein S27-like, a p53-Inducible Modulator of Cell Fate in Response to Genotoxic Stress
Cancer Res., December 1, 2007; 67(23): 11317 - 11326.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. I. van de Wetering and C. M. Knudson
Chromosomal Instability and Supernumerary Centrosomes Represent Precursor Defects in a Mouse Model of T-Cell Lymphoma
Cancer Res., September 1, 2007; 67(17): 8081 - 8088.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
X. Lin and S. B. Howell
DNA mismatch repair and p53 function are major determinants of the rate of development of cisplatin resistance
Mol. Cancer Ther., May 1, 2006; 5(5): 1239 - 1247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Teer, Y. J. Machida, H. Labit, O. Novac, O. Hyrien, K. Marheineke, M. Zannis-Hadjopoulos, and A. Dutta
Proliferating Human Cells Hypomorphic for Origin Recognition Complex 2 and Pre-replicative Complex Formation Have a Defect in p53 Activation and Cdk2 Kinase Activation
J. Biol. Chem., March 10, 2006; 281(10): 6253 - 6260.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Si and E. S. Robertson
Kaposi's Sarcoma-Associated Herpesvirus-Encoded Latency-Associated Nuclear Antigen Induces Chromosomal Instability through Inhibition of p53 Function
J. Virol., January 15, 2006; 80(2): 697 - 709.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. C. Shen, H. Heng, Y. Wang, S. Lu, G. Liu, C.-X. Deng, S.C. Brooks, and Y. A. Wang
ATM and p21 Cooperate to Suppress Aneuploidy and Subsequent Tumor Development
Cancer Res., October 1, 2005; 65(19): 8747 - 8753.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
S.C. Reshmi and S.M. Gollin
Chromosomal Instability in Oral Cancer Cells
Journal of Dental Research, February 1, 2005; 84(2): 107 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Yun, C. Lie-A-Cheong, and A. C. G. Porter
Discriminatory suppression of homologous recombination by p53
Nucleic Acids Res., December 15, 2004; 32(22): 6479 - 6489.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-t. Kim, P. P. Ongusaha, Y.-K. Hong, S. K. Kurdistani, M. Nakamura, K. P. Lu, and S. W. Lee
Function of Drg1/Rit42 in p53-dependent Mitotic Spindle Checkpoint
J. Biol. Chem., September 10, 2004; 279(37): 38597 - 38602.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Pati, B. R. Haddad, A. Haegele, H. Thompson, F. S. Kittrell, A. Shepard, C. Montagna, N. Zhang, G. Ge, S. K. Otta, et al.
Hormone-Induced Chromosomal Instability in p53-Null Mammary Epithelium
Cancer Res., August 15, 2004; 64(16): 5608 - 5616.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. M. Bailey, M. A. Brenneman, and E. H. Goodwin
Frequent recombination in telomeric DNA may extend the proliferative life of telomerase-negative cells
Nucleic Acids Res., July 16, 2004; 32(12): 3743 - 3751.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Kawamura, H. Izumi, Z. Ma, R. Ikeda, M. Moriyama, T. Tanaka, T. Nojima, L. S. Levin, K. Fujikawa-Yamamoto, K. Suzuki, et al.
Induction of Centrosome Amplification and Chromosome Instability in Human Bladder Cancer Cells by p53 Mutation and Cyclin E Overexpression
Cancer Res., July 15, 2004; 64(14): 4800 - 4809.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Myung, G. Ghosh, F. J. Fattah, G. Li, H. Kim, A. Dutia, E. Pak, S. Smith, and E. A. Hendrickson
Regulation of Telomere Length and Suppression of Genomic Instability in Human Somatic Cells by Ku86
Mol. Cell. Biol., June 1, 2004; 24(11): 5050 - 5059.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. Furlan, R. Cerutti, S. Uccella, S. La Rosa, E. Rigoli, A. Genasetti, and C. Capella
Different Molecular Profiles Characterize Well-Differentiated Endocrine Tumors and Poorly Differentiated Endocrine Carcinomas of the Gastroenteropancreatic Tract
Clin. Cancer Res., February 1, 2004; 10(3): 947 - 957.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Cotsiki, R. L. Lock, Y. Cheng, G. L. Williams, J. Zhao, D. Perera, R. Freire, A. Entwistle, E. A. Golemis, T. M. Roberts, et al.
Simian virus 40 large T antigen targets the spindle assembly checkpoint protein Bub1
PNAS, January 27, 2004; 101(4): 947 - 952.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Traverso, C. Bettegowda, J. Kraus, M. R. Speicher, K. W. Kinzler, B. Vogelstein, and C. Lengauer
Hyper-Recombination and Genetic Instability in BLM-Deficient Epithelial Cells
Cancer Res., December 15, 2003; 63(24): 8578 - 8581.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R.-A. Risques, V. Moreno, M. Ribas, E. Marcuello, G. Capella, and M. A. Peinado
Genetic Pathways and Genome-Wide Determinants of Clinical Outcome in Colorectal Cancer
Cancer Res., November 1, 2003; 63(21): 7206 - 7214.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. Ott, H. Vogelsang, J. Mueller, K. Becker, M. Muller, U. Fink, J. R. Siewert, H. Hofler, and G. Keller
Chromosomal Instability Rather Than p53 Mutation Is Associated with Response to Neoadjuvant Cisplatin-based Chemotherapy in Gastric Carcinoma
Clin. Cancer Res., June 1, 2003; 9(6): 2307 - 2315.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. A. Pihan, J. Wallace, Y. Zhou, and S. J. Doxsey
Centrosome Abnormalities and Chromosome Instability Occur Together in Pre-invasive Carcinomas
Cancer Res., March 15, 2003; 63(6): 1398 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Duensing and K. Munger
The Human Papillomavirus Type 16 E6 and E7 Oncoproteins Independently Induce Numerical and Structural Chromosome Instability
Cancer Res., December 1, 2002; 62(23): 7075 - 7082.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Tanaka and J. F.X. Diffley
Deregulated G1-cyclin expression induces genomic instability by preventing efficient pre-RC formation
Genes & Dev., October 15, 2002; 16(20): 2639 - 2649.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2002 by the American Association for Cancer Research.