Cancer Research CTRC-AACR San Antonio Breast Cancer Symposium Translational Medicine Conference in Israel
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hensen, K.
Right arrow Articles by Voz, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hensen, K.
Right arrow Articles by Voz, M. L.
[Cancer Research 62, 1510-1517, March 1, 2002]
© 2002 American Association for Cancer Research

Molecular Biology and Genetics

The Tumorigenic Diversity of the Three PLAG Family Members Is Associated with Different DNA Binding Capacities1

Karen Hensen2, Isabelle C. C. Van Valckenborgh, Koen Kas, Wim J. M. Van de Ven and Marianne L. Voz3

Laboratory for Molecular Oncology, Center for Human Genetics (CME), University of Leuven (KUL) and Flanders Interuniversity Institute for Biotechnology (VIB), Herestraat 49 B-3000 Leuven, Belgium

Pleomorphic adenoma gene (PLAG) 1, the main translocation target in pleomorphic adenomas of the salivary glands, is a member of a new subfamily of zinc finger proteins comprising the tumor suppressor candidate PLAG-like1 (also called ZAC1 or lost on transformation 1) and PLAGL2. In this report, we show that NIH3T3 cells overexpressing PLAG1 or PLAGL2 display the typical markers of neoplastic transformation: (a) the cells lose cell-cell contact inhibition; (b) show anchorage-independent growth; and (c) are able to induce tumors in nude mice. In contrast, PLAGL1 has been shown to prevent the proliferation of tumor cells by inducing cell cycle arrest and apoptosis. This difference in function is also reflected in their DNA binding, as we show here that the three PLAG proteins, although highly homologous in their DNA-binding domain, bind different DNA sequences in a distinct fashion. Interestingly, the PLAG1- and PLAGL2-induced transformation is accompanied by a drastic up-regulation of insulin-like growth factor-II, which we prove is a target of PLAG1 and PLAGL2. This strongly suggests that the oncogenic capacity of PLAG1 and PLAGL2 is mediated at least partly by activating the insulin-like growth factor-II mitogenic pathway.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. C. B. Ammons, D. W. Siemsen, L. K. Nelson-Overton, M. T. Quinn, and K. A. Gauss
Binding of Pleomorphic Adenoma Gene-like 2 to the Tumor Necrosis Factor (TNF)-{alpha}-responsive Region of the NCF2 Promoter Regulates p67phox Expression and NADPH Oxidase Activity
J. Biol. Chem., June 15, 2007; 282(24): 17941 - 17952.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Zheng and Y.-C. Yang
Sumoylation and Acetylation Play Opposite Roles in the Transactivation of PLAG1 and PLAGL2
J. Biol. Chem., December 9, 2005; 280(49): 40773 - 40781.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
Y. Lu, P.-Y. Liu, P. Xiao, and H.-W. Deng
Hotelling's T2 multivariate profiling for detecting differential expression in microarrays
Bioinformatics, July 15, 2005; 21(14): 3105 - 3113.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Declercq, F. Van Dyck, C. V. Braem, I. C. Van Valckenborgh, M. Voz, M. Wassef, L. Schoonjans, B. Van Damme, L. Fiette, and W. J.M. Van de Ven
Salivary Gland Tumors in Transgenic Mice with Targeted PLAG1 Proto-Oncogene Overexpression
Cancer Res., June 1, 2005; 65(11): 4544 - 4553.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. F. Landrette, Y.-H. Kuo, K. Hensen, S. B. van Waalwijk van Doorn-Khosrovani, P. N. Perrat, W. J. M. Van de Ven, R. Delwel, and L. H. Castilla
Plag1 and Plagl2 are oncogenes that induce acute myeloid leukemia in cooperation with Cbfb-MYH11
Blood, April 1, 2005; 105(7): 2900 - 2907.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M.-C. W. Yang, J. C. Weissler, L. S. Terada, F. Deng, and Y.-S. Yang
Pleiomorphic Adenoma Gene-Like-2, a Zinc Finger Protein, Transactivates the Surfactant Protein-C Promoter
Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 35 - 43.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Van Dyck, E. L. D. Delvaux, W. J. M. Van de Ven, and M. V. Chavez
Repression of the Transactivating Capacity of the Oncoprotein PLAG1 by SUMOylation
J. Biol. Chem., August 20, 2004; 279(34): 36121 - 36131.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. H. Castilla, P. Perrat, N. J. Martinez, S. F. Landrette, R. Keys, S. Oikemus, J. Flanegan, S. Heilman, L. Garrett, A. Dutra, et al.
Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia
PNAS, April 6, 2004; 101(14): 4924 - 4929.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2002 by the American Association for Cancer Research.