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Identification of the p33^ING1-regulated Genes that Include Cyclin B1 and Proto-oncogene DEK by Using cDNA Microarray in a Mouse Mammary Epithelial Cell Line NMuMG¹

Division of Biochemistry [M. T., T. O., T. K., T. N., K. W., K. M., M. O., S. H., M. H., A. N.] and Division of Animal Science [H. T.], Chiba Cancer Center Research Institute, Chuoh-ku, Chiba 260-8717; Department of General Surgery, Hokkaido University School of Medicine, Kita-ku, Sapporo 060-8638 [M. T., T. N., K. W., S. H., M. H., S. T.]; Department of Functional Genomics, Chiba University Graduate School of Medicine, Chuo-ku, Chiba 260-8670 [N.S., M.K.]; and Division of Biological Chemistry and Biologicals, National Institute of Health Science, Kamiyoga, Setagaya-ku, Tokyo 158-8501 [H. M., T. H.], Japan

The candidate tumor suppressor p33^ING1 plays an important role in inducinggrowth arrest at G₀-G₁ phase of the cell cycle and/or promoting apoptosis in cancerous cells. p33^ING1 is reported to act as a transcriptional cofactor by associating with tumor suppressor p53, HAT, or histone deacetyltransferase, suggesting that p33^ING1 is involved in chromatin-mediated transcriptional regulation. However, the molecular mechanism of p33^ING1-mediated transcriptional regulation is poorly understood. Here we analyzed expression profiles in mouse mammary epithelial cells (NMuMG) by using a cDNA microarray consisting of 2304 mouse cDNAs after inducing transformation with antisense inhibitor of growth 1 (ING1) in retrovirus vector. The subsequent confirmation of the altered expression levels of the selected genes by semiquantitative reverse transcription-PCR demonstrated that overexpression of the antisense ING1 stimulated expression of 14 genes, which included cyclin B1, 12-O-tetradecanoylphorbol-13-acetate-inducible sequence 11, proto-oncogene DEK, and osteopontin, whereas we have detected transcriptional repression of 5 genes, including TPT1. In addition, adenovirus-mediated overexpression of ING1 in NMuMG cells resulted in down-regulation of cyclin B1, 12-O-tetradecanoylphorbol-13-acetate-inducible sequence 11, DEK, and osteopontin, whereas the levels of TPT1 expression were increased. The further analysis using p53^-/- SAOS2 cells showed that the p33^ING1-induced cyclin B1 down-regulation was p53 dependent. Thus, our cDNA microarray analysis suggested that p33^ING1 targets the multiple genes, including proto-oncogene DEK and cyclin B1, at least some of which are regulated in a p53-dependent manner, in the cells undergoing cell growth or apoptosis.

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