Cancer Research Versailles No Abst Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Langley, R. R.
Right arrow Articles by Fidler, I. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Langley, R. R.
Right arrow Articles by Fidler, I. J.
[Cancer Research 63, 2971-2976, June 1, 2003]
© 2003 American Association for Cancer Research

Tumor Biology

Tissue-specific Microvascular Endothelial Cell Lines from H-2Kb-tsA58 Mice for Studies of Angiogenesis and Metastasis1

Robert R. Langley, Karen M. Ramirez, Rachel Z. Tsan, Melissa Van Arsdall, Monique B. Nilsson and Isaiah J. Fidler2

Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030

Microvascular endothelial cells play a critical role in tumor progression and metastasis by forming capillary networks that encourage tumor growth and by promoting the attachment of circulating tumor cells to the vascular wall of distant tissues. Efforts to study the molecular mechanisms that mediate these complex processes in different anatomical compartments have been impeded by difficulties in the isolation and propagation of endothelial cells from different organs. To overcome these limitations, we used two-color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature-sensitive SV40 large T antigen (H-2Kb-tsA58 mice; ImmortoMice). The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM-1, and proved successful in generating microvascular endothelial cell lines from a number of different organs. When cultured under permissive temperatures (33°C), individual cell lines displayed doubling times consistent with endothelial cells possessing an angiogenic phenotype. The transfer of endothelial cells to nonpermissive temperatures (37°C) resulted in cell differentiation and the induction of a quiescent state. Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1, internalization of acetylated low-density lipoprotein, and formation of loop structures on Matrigel surfaces. The immortalized endothelial cell lines established from H-2Kb-tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
S.-J. Kim, J.-S. Kim, J. Papadopoulos, S. Wook Kim, M. Maya, F. Zhang, J. He, D. Fan, R. Langley, and I. J. Fidler
Circulating Monocytes Expressing CD31: Implications for Acute and Chronic Angiogenesis
Am. J. Pathol., May 1, 2009; 174(5): 1972 - 1980.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. N. Armaiz-Pena, L. S. Mangala, W. A. Spannuth, Y. G. Lin, N. B. Jennings, A. M. Nick, R. R. Langley, R. Schmandt, S. K. Lutgendorf, S. W. Cole, et al.
Estrous Cycle Modulates Ovarian Carcinoma Growth
Clin. Cancer Res., May 1, 2009; 15(9): 2971 - 2978.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Yang, D. Qiao, K. Meyer, and A. Friedl
Signal Transducers and Activators of Transcription Mediate Fibroblast Growth Factor-Induced Vascular Endothelial Morphogenesis
Cancer Res., February 15, 2009; 69(4): 1668 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Giordano, J. Lahdenranta, L. Zhen, U. Chukwueke, I. Petrache, R. R. Langley, I. J. Fidler, R. Pasqualini, R. M. Tuder, and W. Arap
Targeted Induction of Lung Endothelial Cell Apoptosis Causes Emphysema-like Changes in the Mouse
J. Biol. Chem., October 24, 2008; 283(43): 29447 - 29460.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. Zhong, J. Roybal, R. Chaerkady, W. Zhang, K. Choi, C. A. Alvarez, H. Tran, C. J. Creighton, S. Yan, R. M. Strieter, et al.
Identification of Secreted Proteins that Mediate Cell-Cell Interactions in an In vitro Model of the Lung Cancer Microenvironment
Cancer Res., September 1, 2008; 68(17): 7237 - 7245.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Y. Hwang, L. S. Mangala, J. Y. Fok, Y. G. Lin, W. M. Merritt, W. A. Spannuth, A. M. Nick, D. J. Fiterman, P. E. Vivas-Mejia, M. T. Deavers, et al.
Clinical and Biological Significance of Tissue Transglutaminase in Ovarian Carcinoma
Cancer Res., July 15, 2008; 68(14): 5849 - 5858.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Qiao, X. Yang, K. Meyer, and A. Friedl
Glypican-1 Regulates Anaphase Promoting Complex/Cyclosome Substrates and Cell Cycle Progression in Endothelial Cells
Mol. Biol. Cell, July 1, 2008; 19(7): 2789 - 2801.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
W. M. Merritt, Y. G. Lin, W. A. Spannuth, M. S. Fletcher, A. A. Kamat, L. Y. Han, C. N. Landen, N. Jennings, K. De Geest, R. R. Langley, et al.
Effect of Interleukin-8 Gene Silencing With Liposome-Encapsulated Small Interfering RNA on Ovarian Cancer Cell Growth
J Natl Cancer Inst, March 5, 2008; 100(5): 359 - 372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Yao, T. Taguchi, T. Matsumura, R. Pestell, D. Edelstein, I. Giardino, G. Suske, N. Rabbani, P. J. Thornalley, V. P. Sarthy, et al.
High Glucose Increases Angiopoietin-2 Transcription in Microvascular Endothelial Cells through Methylglyoxal Modification of mSin3A
J. Biol. Chem., October 19, 2007; 282(42): 31038 - 31045.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. J. Kim, M. Ravoori, C. N. Landen, A. A. Kamat, L. Y. Han, C. Lu, Y. G. Lin, W. M. Merritt, N. Jennings, W. A. Spannuth, et al.
Antitumor and Antivascular Effects of AVE8062 in Ovarian Carcinoma
Cancer Res., October 1, 2007; 67(19): 9337 - 9345.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
W. Wu, M. S. O'Reilly, R. R. Langley, R. Z. Tsan, C. H. Baker, N. Bekele, X. M. Tang, A. Onn, I. J. Fidler, and R. S. Herbst
Expression of epidermal growth factor (EGF)/transforming growth factor-{alpha} by human lung cancer cells determines their response to EGF receptor tyrosine kinase inhibition in the lungs of mice
Mol. Cancer Ther., October 1, 2007; 6(10): 2652 - 2663.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
S. Kim, Y. D. Yazici, G. Calzada, Z.-Y. Wang, M. N. Younes, S. A. Jasser, A. K. El-Naggar, and J. N. Myers
Sorafenib inhibits the angiogenesis and growth of orthotopic anaplastic thyroid carcinoma xenografts in nude mice
Mol. Cancer Ther., June 1, 2007; 6(6): 1785 - 1792.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
R. R. Langley and I. J. Fidler
Tumor Cell-Organ Microenvironment Interactions in the Pathogenesis of Cancer Metastasis
Endocr. Rev., May 1, 2007; 28(3): 297 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Hara, S. Samuel, J. Liu, D. Rosen, R. R. Langley, and H. Naora
A Homeobox Gene Related to Drosophila Distal-Less Promotes Ovarian Tumorigenicity by Inducing Expression of Vascular Endothelial Growth Factor and Fibroblast Growth Factor-2
Am. J. Pathol., May 1, 2007; 170(5): 1594 - 1606.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Lu, T. Bonome, Y. Li, A. A. Kamat, L. Y. Han, R. Schmandt, R. L. Coleman, D. M. Gershenson, R. B. Jaffe, M. J. Birrer, et al.
Gene Alterations Identified by Expression Profiling in Tumor-Associated Endothelial Cells from Invasive Ovarian Carcinoma
Cancer Res., February 15, 2007; 67(4): 1757 - 1768.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
W. Wu, A. Onn, T. Isobe, S. Itasaka, R. R. Langley, T. Shitani, K. Shibuya, R. Komaki, A. J. Ryan, I. J. Fidler, et al.
Targeted therapy of orthotopic human lung cancer by combined vascular endothelial growth factor and epidermal growth factor receptor signaling blockade
Mol. Cancer Ther., February 1, 2007; 6(2): 471 - 483.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. A. Kamat, T. J. Kim, C. N. Landen Jr., C. Lu, L. Y. Han, Y. G. Lin, W. M. Merritt, P. H. Thaker, D. M. Gershenson, F. Z. Bischoff, et al.
Metronomic Chemotherapy Enhances the Efficacy of Antivascular Therapy in Ovarian Cancer
Cancer Res., January 1, 2007; 67(1): 281 - 288.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
C. N. Landen Jr., C. Lu, L. Y. Han, K. T. Coffman, E. Bruckheimer, J. Halder, L. S. Mangala, W. M. Merritt, Y. G. Lin, C. Gao, et al.
Efficacy and Antivascular Effects of EphA2 Reduction With an Agonistic Antibody in Ovarian Cancer.
J Natl Cancer Inst, November 1, 2006; 98(21): 1558 - 1570.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. Halder, A. A. Kamat, C. N. Landen Jr., L. Y. Han, S. K. Lutgendorf, Y. G. Lin, W. M. Merritt, N. B. Jennings, A. Chavez-Reyes, R. L. Coleman, et al.
Focal Adhesion Kinase Targeting Using In vivo Short Interfering RNA Delivery in Neutral Liposomes for Ovarian Carcinoma Therapy.
Clin. Cancer Res., August 15, 2006; 12(16): 4916 - 4924.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
S.-J. Kim, H. Uehara, S. Yazici, J. E. Busby, T. Nakamura, J. He, M. Maya, C. Logothetis, P. Mathew, X. Wang, et al.
Targeting platelet-derived growth factor receptor on endothelial cells of multidrug-resistant prostate cancer.
J Natl Cancer Inst, June 7, 2006; 98(11): 783 - 793.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Wislez, N. Fujimoto, J. G. Izzo, A. E. Hanna, D. D. Cody, R. R. Langley, H. Tang, M. D. Burdick, M. Sato, J. D. Minna, et al.
High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
Cancer Res., April 15, 2006; 66(8): 4198 - 4207.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. M.A. Rouschop, N. Claessen, S. T. Pals, J. J. Weening, and S. Florquin
CD44 Disruption Prevents Degeneration of the Capillary Network in Obstructive Nephropathy via Reduction of TGF-beta1-Induced Apoptosis
J. Am. Soc. Nephrol., March 1, 2006; 17(3): 746 - 753.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. B. Nilsson, R. R. Langley, and I. J. Fidler
Interleukin-6, Secreted by Human Ovarian Carcinoma Cells, Is a Potent Proangiogenic Cytokine
Cancer Res., December 1, 2005; 65(23): 10794 - 10800.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
J. M. Summy, J. G. Trevino, D. P. Lesslie, C. H. Baker, W. C. Shakespeare, Y. Wang, R. Sundaramoorthi, C. A. Metcalf III, J. A. Keats, T. K. Sawyer, et al.
AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes
Mol. Cancer Ther., December 1, 2005; 4(12): 1900 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
P. H. Thaker, S. Yazici, M. B. Nilsson, K. Yokoi, R. Z. Tsan, J. He, S.-J. Kim, I. J. Fidler, and A. K. Sood
Antivascular Therapy for Orthotopic Human Ovarian Carcinoma through Blockade of the Vascular Endothelial Growth Factor and Epidermal Growth Factor Receptors
Clin. Cancer Res., July 1, 2005; 11(13): 4923 - 4933.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Kilic, L. Oliveira-Ferrer, J.-H. Wurmbach, S. Loges, F. Chalajour, S. N. Vahid, J. Weil, M. Fernando, and S. Ergun
Pro-angiogenic Signaling by the Endothelial Presence of CEACAM1
J. Biol. Chem., January 21, 2005; 280(3): 2361 - 2369.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. R. Langley, D. Fan, R. Z. Tsan, R. Rebhun, J. He, S.-J. Kim, and I. J. Fidler
Activation of the Platelet-Derived Growth Factor-Receptor Enhances Survival of Murine Bone Endothelial Cells
Cancer Res., June 1, 2004; 64(11): 3727 - 3730.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Pasqualini and W. Arap
Hybridoma-free generation of monoclonal antibodies
PNAS, January 6, 2004; 101(1): 257 - 259.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2003 by the American Association for Cancer Research.