Cancer Research Cell Death Mechanisms and Cancer Therapy Sign up for Cancer Research eTOC's
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Zhang, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Zhang, W.
[Cancer Research 63, 4315-4321, August 1, 2003]
© 2003 American Association for Cancer Research

Advances in Brief

Insulin-like Growth Factor Binding Protein 2 Enhances Glioblastoma Invasion by Activating Invasion-enhancing Genes1

Hua Wang, Huamin Wang, Weiping Shen, Helen Huang, Limei Hu, Latha Ramdas, Yi-Hong Zhou, Warren S-L. Liao, Gregory N. Fuller and Wei Zhang2

Departments of Pathology [H. W., W. S., L. H., L. R., G. N. F., W. Z.], Biochemistry and Molecular Biology [W. L., H. H.], and Neuro-Oncology [Y. Z.], The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030 and Department of Pathology, The University of Texas-Houston Medical School, Houston, Texas 77030 [H. W.]

Comparison of gene expressing profiles between gliomas with different grades revealed frequent overexpression of insulin-like growth factor binding protein 2 (IGFBP2) in glioblastoma (GBM), the most advanced stage of glioma. To determine whether IGFBP2 is involved in the proliferative and invasive nature of GBM, we established stable SNB19 GBM cell lines that overexpress IGFBP2. Although there was no marked difference in the cell growth between IGFBP2 overexpressing SNB19(BP2) lines when compared with the control cells, these clones showed significantly increased invasive rates when compared with the parental or vector transfected SNB19 cells. Total RNAs from controls and SNB19(BP2) clones were used for microarray analysis to detect IGFBP2-mediated alterations in gene expression. When compared with parental or vector-transfected control cells, SNB19(BP2) cells consistently showed 3–5-fold increase in the expression of matrix metalloproteinase-2 (MMP-2) as well as other invasion related genes. Increased MMP-2 expression in SNB19(BP2) cells was subsequently confirmed by real time reverse-transcription PCR, Western blotting, and gelatin zymography. Furthermore, consistent with increased MMP-2 expression in SNB19(BP2) cells, transient transfection of a MMP-2 promoter/luciferase reporter also resulted in 3–6-fold higher luciferase activity in SNB19(BP2) cells than in parental or vector-transfected control cells. Finally, tissue microarray analysis of 68 GBM tissue specimens showed a significant correlation between the overexpression of IGFBP2 and elevated MMP-2 expression. Taken together, our data provide evidence that IGFBP2 contributes to glioma progression in part by enhancing MMP-2 gene transcription and in turn tumor cell invasion.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. M. Moore, K. M. Holmes, S. M. Smith, Y. Wu, E. Tchougounova, L. Uhrbom, R. Sawaya, J. M. Bruner, G. N. Fuller, and W. Zhang
IGFBP2 is a candidate biomarker for Ink4a-Arf status and a therapeutic target for high-grade gliomas
PNAS, September 29, 2009; 106(39): 16675 - 16679.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. H. Park, B.-H. Ahn, Y.-K. Hong, and D. S. Min
Overexpression of phospholipase D enhances matrix metalloproteinase-2 expression and glioma cell invasion via protein kinase C and protein kinase A/NF-{kappa}B/Sp1-mediated signaling pathways
Carcinogenesis, February 1, 2009; 30(2): 356 - 365.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Wang, X. Song, C. Logsdon, G. Zhou, D. B. Evans, J. L. Abbruzzese, S. R. Hamilton, T.-H. Tan, and H. Wang
Proteasome-Mediated Degradation and Functions of Hematopoietic Progenitor Kinase 1 in Pancreatic Cancer
Cancer Res., February 1, 2009; 69(3): 1063 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 Neuro Oncol DukeHome page
Y. Lin, T. Jiang, K. Zhou, L. Xu, B. Chen, G. Li, X. Qiu, T. Jiang, W. Zhang, and S. W. Song
Plasma IGFBP-2 levels predict clinical outcomes of patients with high-grade gliomas
Neuro-oncol, January 1, 2009; 11(5): 468 - 476.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. I. So, R. J. Levitt, B. Eigl, L. Fazli, M. Muramaki, S. Leung, M. C.U. Cheang, T. O. Nielsen, M. Gleave, and M. Pollak
Insulin-Like Growth Factor Binding Protein-2 Is a Novel Therapeutic Target Associated with Breast Cancer
Clin. Cancer Res., November 1, 2008; 14(21): 6944 - 6954.
[Abstract] [Full Text] [PDF]

Home page
 J Child NeurolHome page
O. J. Becher, K. M. Peterson, S. Khatua, M. R. Santi, and T. J. MacDonald
IGFBP2 Is Overexpressed by Pediatric Malignant Astrocytomas and Induces the Repair Enzyme DNA-PK
J Child Neurol, October 1, 2008; 23(10): 1205 - 1213.
[Abstract] [PDF]

Home page
 J Biomol ScreenHome page
C.-L. Chang
Genome-Wide Oligonucleotide Microarray Analysis of Gene-Expression Profiles of Taiwanese Patients with Anaplastic Astrocytoma and Glioblastoma Multiforme
J Biomol Screen, October 1, 2008; 13(9): 912 - 921.
[Abstract] [PDF]

Home page
 Cancer Res.Home page
A. M. Cervera, N. Apostolova, F. L. Crespo, M. Mata, and K. J. McCreath
Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Cancer Res., June 1, 2008; 68(11): 4058 - 4067.
[Abstract] [Full Text] [PDF]

Home page
 Neuro Oncol DukeHome page
A. Nakamizo, T. Amano, W. Zhang, X.-Q. Zhang, L. Ramdas, T.-J. Liu, B. N. Bekele, T. Shono, T. Sasaki, W. F. Benedict, et al.
Phosphorylation of Thr18 and Ser20 of p53 in Ad-p53-induced apoptosis
Neuro-oncol, January 1, 2008; 10(3): 275 - 291.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Shirahata, K. Iwao-Koizumi, S. Saito, N. Ueno, M. Oda, N. Hashimoto, J. A. Takahashi, and K. Kato
Gene Expression-Based Molecular Diagnostic System for Malignant Gliomas Is Superior to Histological Diagnosis
Clin. Cancer Res., December 15, 2007; 13(24): 7341 - 7356.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F. B. Furnari, T. Fenton, R. M. Bachoo, A. Mukasa, J. M. Stommel, A. Stegh, W. C. Hahn, K. L. Ligon, D. N. Louis, C. Brennan, et al.
Malignant astrocytic glioma: genetics, biology, and paths to treatment
Genes & Dev., November 1, 2007; 21(21): 2683 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Fukushima, T. Tezuka, T. Shimomura, S. Nakano, and H. Kataoka
Silencing of Insulin-like Growth Factor-binding Protein-2 in Human Glioblastoma Cells Reduces Both Invasiveness and Expression of Progression-associated Gene CD24
J. Biol. Chem., June 22, 2007; 282(25): 18634 - 18644.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Mehrian-Shai, C. D. Chen, T. Shi, S. Horvath, S. F. Nelson, J. K. V. Reichardt, and C. L. Sawyers
Insulin growth factor-binding protein 2 is a candidate biomarker for PTEN status and PI3K/Akt pathway activation in glioblastoma and prostate cancer
PNAS, March 27, 2007; 104(13): 5563 - 5568.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. A. Mayes, Y. Hu, Y. Teng, E. Siegel, X. Wu, K. Panda, F. Tan, W.K. A. Yung, and Y.-H. Zhou
PAX6 Suppresses the Invasiveness of Glioblastoma Cells and the Expression of the Matrix Metalloproteinase-2 Gene.
Cancer Res., October 15, 2006; 66(20): 9809 - 9817.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C.-M. Park, M.-J. Park, H.-J. Kwak, H.-C. Lee, M.-S. Kim, S.-H. Lee, I.-C. Park, C. H. Rhee, and S.-I. Hong
Ionizing Radiation Enhances Matrix Metalloproteinase-2 Secretion and Invasion of Glioma Cells through Src/Epidermal Growth Factor Receptor-Mediated p38/Akt and Phosphatidylinositol 3-Kinase/Akt Signaling Pathways.
Cancer Res., September 1, 2006; 66(17): 8511 - 8519.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
K. W Frommer, K. Reichenmiller, B. S Schutt, A. Hoeflich, M. B Ranke, G. Dodt, and M. W Elmlinger
IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T.
J. Mol. Endocrinol., August 1, 2006; 37(1): 13 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. K. Wang, L. Hu, G. N. Fuller, and W. Zhang
An Interaction between Insulin-like Growth Factor-binding Protein 2 (IGFBP2) and Integrin {alpha}5 Is Essential for IGFBP2-induced Cell Mobility
J. Biol. Chem., May 19, 2006; 281(20): 14085 - 14091.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
Y. Zhou, J. Zhang, Q. Liu, R. Bell, D. A. Muruve, P. Forsyth, M. Arcellana-Panlilio, S. Robbins, and V.W. Yong
The chemokine GRO-{alpha} (CXCL1) confers increased tumorigenicity to glioma cells
Carcinogenesis, December 1, 2005; 26(12): 2058 - 2068.
[Abstract] [Full Text] [PDF]

Home page
 Neuro Oncol DukeHome page
Y.-H. Zhou, K. R. Hess, L. Liu, Mark. E. Linskey, and W.K. A. Yung
Modeling prognosis for patients with malignant astrocytic gliomas: Quantifying the expression of multiple genetic markers and clinical variables
Neuro-oncol, October 1, 2005; 7(4): 485 - 494.
[Abstract] [PDF]

Home page
 Cancer Res.Home page
M. Bredel, C. Bredel, D. Juric, G. R. Harsh, H. Vogel, L. D. Recht, and B. I. Sikic
Functional Network Analysis Reveals Extended Gliomagenesis Pathway Maps and Three Novel MYC-Interacting Genes in Human Gliomas
Cancer Res., October 1, 2005; 65(19): 8679 - 8689.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. W. Song, G. N. Fuller, H. Zheng, and W. Zhang
Inactivation of the Invasion Inhibitory Gene IIp45 by Alternative Splicing in Gliomas
Cancer Res., May 1, 2005; 65(9): 3562 - 3567.
[Abstract] [Full Text] [PDF]

Home page
 Neuro Oncol DukeHome page
B. Suarez-Merino, M. Hubank, T. Revesz, W. Harkness, R. Hayward, D. Thompson, J. L. Darling, D. G.T. Thomas, and T. J. Warr
Microarray analysis of pediatric ependymoma identifies a cluster of 112 candidate genes including four transcripts at 22q12.1-q13.3
Neuro-oncol, January 1, 2005; 7(1): 20 - 31.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
P. La, R. W. Schnepp, C. D. Petersen, A. C. Silva, and X. Hua
Tumor Suppressor Menin Regulates Expression of Insulin-Like Growth Factor Binding Protein 2
Endocrinology, July 1, 2004; 145(7): 3443 - 3450.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Song, G. N. Fuller, A. Khan, S. Kong, W. Shen, E. Taylor, L. Ramdas, F. F. Lang, and W. Zhang
IIp45, an insulin-like growth factor binding protein 2 (IGFBP-2) binding protein, antagonizes IGFBP-2 stimulation of glioma cell invasion
PNAS, November 25, 2003; 100(24): 13970 - 13975.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2003 by the American Association for Cancer Research.