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The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada [S. K. S., I. D. C., M. T., V. E. B., P. B. D.], and Program in Developmental Biology [S. K. S., I. D. C., M. T., V. E. B., P. B. D.], Division of Neurosurgery [S. K. S., P. B. D.], Department of Pediatric Laboratory Medicine [C. H.], and Department of Laboratory Medicine and Pathobiology [J. S.], University of Toronto, Toronto, Ontario M5G 1X8 Canada
Most current research on human brain tumors is focused on the molecular and cellular analysis of the bulk tumor mass. However, there is overwhelming evidence in some malignancies that the tumor clone is heterogeneous with respect to proliferation and differentiation. In human leukemia, the tumor clone is organized as a hierarchy that originates from rare leukemic stem cells that possess extensive proliferative and self-renewal potential, and are responsible for maintaining the tumor clone. We report here the identification and purification of a cancer stem cell from human brain tumors of different phenotypes that possesses a marked capacity for proliferation, self-renewal, and differentiation. The increased self-renewal capacity of the brain tumor stem cell (BTSC) was highest from the most aggressive clinical samples of medulloblastoma compared with low-grade gliomas. The BTSC was exclusively isolated with the cell fraction expressing the neural stem cell surface marker CD133. These CD133+ cells could differentiate in culture into tumor cells that phenotypically resembled the tumor from the patient. The identification of a BTSC provides a powerful tool to investigate the tumorigenic process in the central nervous system and to develop therapies targeted to the BTSC.
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J. C. Liu, T. Deng, R. S. Lehal, J. Kim, and E. Zacksenhaus Identification of Tumorsphere- and Tumor-Initiating Cells in HER2/Neu-Induced Mammary Tumors Cancer Res., September 15, 2007; 67(18): 8671 - 8681. [Abstract] [Full Text] [PDF] |
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U. Ganapati, H. T. Tan, M. Lynch, M. Dolezal, S. de Vos, and J. C. Gasson Modeling Notch Signaling in Normal and Neoplastic Hematopoiesis: Global Gene Expression Profiling in Response to Activated Notch Expression Stem Cells, August 1, 2007; 25(8): 1872 - 1880. [Abstract] [Full Text] [PDF] |
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M. Yanagisawa and R. K Yu The expression and functions of glycoconjugates in neural stem cells Glycobiology, July 1, 2007; 17(7): 57R - 74R. [Abstract] [Full Text] [PDF] |
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A. J. Ghods, D. Irvin, G. Liu, X. Yuan, I. R. Abdulkadir, P. Tunici, B. Konda, S. Wachsmann-Hogiu, K. L. Black, and J. S. Yu Spheres Isolated from 9L Gliosarcoma Rat Cell Line Possess Chemoresistant and Aggressive Cancer Stem-Like Cells Stem Cells, July 1, 2007; 25(7): 1645 - 1653. [Abstract] [Full Text] [PDF] |
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C. V. Pfenninger, T. Roschupkina, F. Hertwig, D. Kottwitz, E. Englund, J. Bengzon, S. E. Jacobsen, and U. A. Nuber CD133 Is Not Present on Neurogenic Astrocytes in the Adult Subventricular Zone, but on Embryonic Neural Stem Cells, Ependymal Cells, and Glioblastoma Cells Cancer Res., June 15, 2007; 67(12): 5727 - 5736. [Abstract] [Full Text] [PDF] |
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K. Kato, M. Yoshimoto, K. Kato, S. Adachi, A. Yamayoshi, T. Arima, K. Asanoma, S. Kyo, T. Nakahata, and N. Wake Characterization of side-population cells in human normal endometrium Hum. Reprod., May 1, 2007; 22(5): 1214 - 1223. [Abstract] [Full Text] [PDF] |
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C. Folkins, S. Man, P. Xu, Y. Shaked, D. J. Hicklin, and R. S. Kerbel Anticancer Therapies Combining Antiangiogenic and Tumor Cell Cytotoxic Effects Reduce the Tumor Stem-Like Cell Fraction in Glioma Xenograft Tumors Cancer Res., April 15, 2007; 67(8): 3560 - 3564. [Abstract] [Full Text] [PDF] |
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X. Zheng, G. Shen, X. Yang, and W. Liu Most C6 Cells Are Cancer Stem Cells: Evidence from Clonal and Population Analyses Cancer Res., April 15, 2007; 67(8): 3691 - 3697. [Abstract] [Full Text] [PDF] |
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