This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsumoto, N. Articles by Okayasu, I. Search for Related Content PubMed PubMed Citation Articles by Matsumoto, N. Articles by Okayasu, I.

High Epithelial and Stromal Genetic Instability of Chromosome 17 in Ulcerative Colitis-associated Carcinogenesis¹

Department of Pathology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan

To define the relative frequencies of genetic instability in stromal and epithelial compartments during ulcerative colitis (UC)-associated tumorigenesis, samples from laser-captured microdissection were assessed for microsatellite instability and loss of heterozygosity in regenerative tissue, dysplasia, and carcinomas in long-standing UC cases. Five National Cancer Institute-recommended standard markers and four markers located close to p53 and BRCA1 genes in chromosome 17 were tested, and p53 gene sequencing was also carried out. Although chromosome 17-MSI and -loss of heterozygosity in epithelium correlated with histological progression, in stroma they showed a consistently high frequency throughout the different stages, indicating a distinct carcinogenesis pathway of UC. The rates for standard markers were lower in both epithelium and stroma.

This article has been cited by other articles:

				Y. Bian, T. J. Knobloch, M. Sadim, V. Kaklamani, A. Raji, G.-Y. Yang, C. M. Weghorst, and B. Pasche Somatic acquisition of TGFBR1*6A by epithelial and stromal cells during head and neck and colon cancer development Hum. Mol. Genet., December 15, 2007; 16(24): 3128 - 3135. [Abstract] [Full Text] [PDF]

				K Ishiguro, T Yoshida, H Yagishita, Y Numata, and T Okayasu Epithelial and stromal genetic instability contributes to genesis of colorectal adenomas Gut, May 1, 2006; 55(5): 695 - 702. [Abstract] [Full Text] [PDF]