This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gröhn, O. H. J. Articles by Garwood, M. Search for Related Content PubMed PubMed Citation Articles by Gröhn, O. H. J. Articles by Garwood, M.

Novel Magnetic Resonance Imaging Contrasts for Monitoring Response to Gene Therapy in Rat Glioma

¹ Department of Biomedical NMR and National Bio NMR Facility,
² Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland;
³ Center for Magnetic Resonance Research, Department of Radiology, and Cancer Center, University of Minnesota, Minneapolis, Minnesota;
⁴ School of Biological Sciences, University of Manchester, Manchester, United Kingdom

Magnetic resonance imaging relaxation times, T₁ and Carr-Purcell T₂ (CP-T₂), were measured in a glioma herpes simplex virus-thymidine kinase gene therapy model. In treated tumors with >50% cell death by histology, T₁ and CP-T₂ measured with short spacing (_CP) between centers of adiabatic refocusing pulses showed similar enhanced sensitivity to cytotoxic cell damage over CP-T₂ measured with long _CP (long-_CP T₂: 54.3 ± 0.7 and 55.4 ± 1.2 ms, P = 0.30; short-_CP T₂: 61.3 ± 1.0 and 64.2 ± 1.1 ms, P < 0.05 before and day 2 of treatment, respectively). Without treatment, long-_CP T₂ provided the most pronounced contrast between tumor and normal cerebral tissue. These data demonstrate that endogenous T₂ contrast can be modulated and extended in a manner likely to be clinically important.

This article has been cited by other articles:

				M. I. Kettunen, A. Sierra, M. J. Narvainen, P. K. Valonen, S. Yla-Herttuala, R. A. Kauppinen, and O. H. J. Grohn Low Spin-Lock Field T1 Relaxation in the Rotating Frame as a Sensitive MR Imaging Marker for Gene Therapy Treatment Response in Rat Glioma Radiology, March 1, 2007; 243(3): 796 - 803. [Abstract] [Full Text] [PDF]