Cancer Research Infection and Cancer: Biology, Therapeutics, and Prevention
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pálmer, H. G.
Right arrow Articles by Muñoz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pálmer, H. G.
Right arrow Articles by Muñoz, A.
[Cancer Research 63, 7799-7806, November 15, 2003]
© 2003 American Association for Cancer Research

Regular Articles

Genetic Signatures of Differentiation Induced by 1{alpha},25-Dihydroxyvitamin D3 in Human Colon Cancer Cells

Héctor G. Pálmer1, Marta Sánchez-Carbayo2, Paloma Ordóñez-Morán1, María Jesús Larriba1, Carlos Cordón-Cardó2 and Alberto Muñoz1

1 Instituto de Investigaciones Biomédicas "Alberto Sols," Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Madrid, Spain,
2 Division of Molecular Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York

Epidemiological and preclinical data indicate that vitamin D and its most active metabolite 1{alpha},25-dihydroxyvitamin D3 [1{alpha},25(OH)2D3] have anticancer activity. Accordingly, clinical trials are under way using several nonhypercalcemic 1{alpha},25(OH)2D3 analogues against various neoplasms including colon cancer. 1{alpha},25(OH)2D3 induces proliferation arrest and epithelial differentiation of human SW480-ADH colon cancer cells. We examined the gene expression profiles associated with 1{alpha},25(OH)2D3 exposure using oligonucleotide microarrays. 1{alpha},25(OH)2D3 changed the expression levels of numerous previously unreported genes, including many involved in transcription, cell adhesion, DNA synthesis, apoptosis, redox status, and intracellular signaling. Most genes were up-regulated, and only a small fraction were down-regulated. Fourteen of 17 candidate genes studied were validated as 1{alpha},25(OH)2D3 target genes by Northern and Western blotting or immunocytochemistry. They included c-JUN, JUNB, JUND, FREAC-1/FoxF1, ZNF-44/KOX7, plectin, filamin, keratin-13, G0S2, and the putative tumor suppressors NES-1 and protease M. There was little overlap between genes regulated after short (4 h) or long (48 h) exposure. Gene regulatory effects of 1{alpha},25(OH)2D3 in SW480-ADH cells differed from those in LS-174T cells, which lack E-cadherin and do not differentiate in response to 1{alpha},25(OH)2D3. Data from this study reveal that 1{alpha},25(OH)2D3 causes a profound change in gene expression profiles and provide a mechanistic basis to the ongoing clinical studies using nonhypercalcemic vitamin D3 derivatives for colon cancer prevention and treatment.




This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
J. P. Vaque, B. Fernandez-Garcia, P. Garcia-Sanz, N. Ferrandiz, G. Bretones, F. Calvo, P. Crespo, M. C. Marin, and J. Leon
c-Myc Inhibits Ras-Mediated Differentiation of Pheochromocytoma Cells by Blocking c-Jun Up-Regulation
Mol. Cancer Res., February 1, 2008; 6(2): 325 - 339.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
O. Aguilera, C. Pena, J. M. Garcia, M. J. Larriba, P. Ordonez-Moran, D. Navarro, A. Barbachano, I. Lopez de Silanes, E. Ballestar, M. F. Fraga, et al.
The Wnt antagonist DICKKOPF-1 gene is induced by 1{alpha},25-dihydroxyvitamin D3 associated to the differentiation of human colon cancer cells
Carcinogenesis, September 1, 2007; 28(9): 1877 - 1884.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
L. Nonn, D. Duong, and D. M. Peehl
Chemopreventive anti-inflammatory activities of curcumin and other phytochemicals mediated by MAP kinase phosphatase-5 in prostate cells
Carcinogenesis, June 1, 2007; 28(6): 1188 - 1196.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-N. Zhu, H.-J. He, S. Kole, T. D'Souza, R. Agarwal, P. J. Morin, and M. Bernier
Filamin A-mediated Down-regulation of the Exchange Factor Ras-GRF1 Correlates with Decreased Matrix Metalloproteinase-9 Expression in Human Melanoma Cells
J. Biol. Chem., May 18, 2007; 282(20): 14816 - 14826.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Maruyama, F. Aoki, M. Toyota, Y. Sasaki, H. Akashi, H. Mita, H. Suzuki, K. Akino, M. Ohe-Toyota, Y. Maruyama, et al.
Comparative Genome Analysis Identifies the Vitamin D Receptor Gene as a Direct Target of p53-Mediated Transcriptional Activation.
Cancer Res., May 1, 2006; 66(9): 4574 - 4583.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. Nonn, L. Peng, D. Feldman, and D. M. Peehl
Inhibition of p38 by Vitamin D Reduces Interleukin-6 Production in Normal Prostate Cells via Mitogen-Activated Protein Kinase Phosphatase 5: Implications for Prostate Cancer Prevention by Vitamin D.
Cancer Res., April 15, 2006; 66(8): 4516 - 4524.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhang, P. Li, J. Bao, S. V. Nicosia, H. Wang, S. A. Enkemann, and W. Bai
Suppression of Death Receptor-mediated Apoptosis by 1,25-Dihydroxyvitamin D3 Revealed by Microarray Analysis
J. Biol. Chem., October 21, 2005; 280(42): 35458 - 35468.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Matilainen, M. Malinen, K. Saavalainen, and C. Carlberg
Regulation of multiple insulin-like growth factor binding protein genes by 1{alpha},25-dihydroxyvitamin D3
Nucleic Acids Res., September 26, 2005; 33(17): 5521 - 5532.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. L. M. Sutton, X. Zhang, T. I. Ellison, and P. N. MacDonald
The 1,25(OH)2D3-Regulated Transcription Factor MN1 Stimulates Vitamin D Receptor-Mediated Transcription and Inhibits Osteoblastic Cell Proliferation
Mol. Endocrinol., September 1, 2005; 19(9): 2234 - 2244.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
S. Nagpal, S. Na, and R. Rathnachalam
Noncalcemic Actions of Vitamin D Receptor Ligands
Endocr. Rev., August 1, 2005; 26(5): 662 - 687.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Sinkkonen, M. Malinen, K. Saavalainen, S. Vaisanen, and C. Carlberg
Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter
Nucleic Acids Res., April 29, 2005; 33(8): 2440 - 2451.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. Adachi, Y. Honma, H. Masuno, K. Kawana, I. Shimomura, S. Yamada, and M. Makishima
Selective activation of vitamin D receptor by lithocholic acid acetate, a bile acid derivative
J. Lipid Res., January 1, 2005; 46(1): 46 - 57.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. P. B. Dackiw, S. Ezzat, P. Huang, W. Liu, and S. L. Asa
Vitamin D3 Administration Induces Nuclear p27 Accumulation, Restores Differentiation, and Reduces Tumor Burden in a Mouse Model of Metastatic Follicular Thyroid Cancer
Endocrinology, December 1, 2004; 145(12): 5840 - 5846.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
S. R. Boutin, A. B. Rogers, Zeli Shen, R. C. Fry, J. A. Love, P. R. Nambiar, S. Suerbaum, and J. G. Fox
Hepatic Temporal Gene Expression Profiling in Helicobacter hepaticus-Infected A/JCr Mice
Toxicol Pathol, October 1, 2004; 32(6): 678 - 693.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2003 by the American Association for Cancer Research.