| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
B p50 Homodimer/Bcl-3 Complexes in Nasopharyngeal Carcinoma
1 Department of Microbiology-Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina;
2 Subang Jaya Medical Centre, Selangor DE, Malaysia; and
3 Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
EBV latent infection is associated with the development of lymphoid and epithelial malignancies such as nasopharyngeal carcinoma (NPC). The EBV latent membrane protein 1 (LMP1) acts as a constitutively active tumor necrosis factor receptor and activates cellular signaling pathways such as c-Jun-NH2-terminal kinase, cdc42, Akt, and nuclear factor (NF)-
B. In epithelial cells, two regions of LMP1 induce specific forms of NF-B. COOH-terminal activating region 2 only activates p52/p65 dimers, whereas COOH-terminal activating region 1 activates p50/p50, p50/p52, and p52/p65 dimers and also uniquely up-regulates the epidermal growth factor receptor (EGFR) at the mRNA level. Deregulation of specific NF-B members is associated with the development of many cancers. In this study, the status of NF-B activation was investigated in NPC to determine which NF-B dimers may contribute to the development of NPC. Electrophoretic mobility shift assay, immunoblot, ELISA, and immunohistochemistry data demonstrate that in NPC, NF-B p50 homodimers are specifically activated, and this activation is not dependent on LMP1 expression. Coimmunoprecipitation assays indicate that homodimers are bound to the transcriptional coactivator Bcl-3, and chromatin immunoprecipitation indicates that this complex is bound to NF-B consensus motifs within the egfr promoter in NPC. The discrete yet striking NF-B p50 activation in NPC suggests that p50/p50 homodimers may be important factors in the development of NPC and may contribute to oncogenesis through transcriptional up-regulation of target genes through their interaction with Bcl-3.
This article has been cited by other articles:
|
|
 |
|
  P. Johansson, A. Jansson, U. Ruetschi, and L. Rymo Nuclear Factor-{kappa}B Binds to the Epstein-Barr Virus LMP1 Promoter and Upregulates Its Expression J. Virol., February 1, 2009; 83(3): 1393 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Kim, N. Sharma, and J. K. Nyborg The Proto-Oncogene Bcl3, Induced by Tax, Represses Tax-Mediated Transcription via p300 Displacement from the Human T-Cell Leukemia Virus Type 1 Promoter J. Virol., December 1, 2008; 82(23): 11939 - 11947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Kuo, P.-Y. Yeh, L.-T. Chen, M.-S. Wu, C.-W. Lin, K.-H. Yeh, Y.-S. Tzeng, J.-Y. Chen, P.-N. Hsu, J.-T. Lin, et al. Overexpression of B cell-activating factor of TNF family (BAFF) is associated with Helicobacter pylori-independent growth of gastric diffuse large B-cell lymphoma with histologic evidence of MALT lymphoma Blood, October 1, 2008; 112(7): 2927 - 2934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H.Y. Shair, C. I. Schnegg, and N. Raab-Traub EBV Latent Membrane Protein 1 Effects on Plakoglobin, Cell Growth, and Migration Cancer Res., September 1, 2008; 68(17): 6997 - 7005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-P. Kung and N. Raab-Traub Epstein-Barr Virus Latent Membrane Protein 1 Induces Expression of the Epidermal Growth Factor Receptor through Effects on Bcl-3 and STAT3 J. Virol., June 1, 2008; 82(11): 5486 - 5493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takami, H. Nakagami, R. Morishita, T. Katsuya, H. Hayashi, M. Mori, H. Koriyama, Y. Baba, O. Yasuda, H. Rakugi, et al. Potential Role of CYLD (Cylindromatosis) as a Deubiquitinating Enzyme in Vascular Cells Am. J. Pathol., March 1, 2008; 172(3): 818 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Thornburg and N. Raab-Traub Induction of Epidermal Growth Factor Receptor Expression by Epstein-Barr Virus Latent Membrane Protein 1 C-Terminal-Activating Region 1 Is Mediated by NF-{kappa}B p50 Homodimer/Bcl-3 Complexes J. Virol., December 1, 2007; 81(23): 12954 - 12961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-M. Chen, T. E. Spratt, B. A. Stanley, D. A. De Cotiis, M. C. Bewley, J. M. Flanagan, D. Desai, A. Das, E. S. Fiala, S. Amin, et al. Inhibition of Nuclear Factor-{kappa}B DNA Binding by Organoselenocyanates through Covalent Modification of the p50 Subunit Cancer Res., November 1, 2007; 67(21): 10475 - 10483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Mainou, D. N. Everly Jr., and N. Raab-Traub Unique Signaling Properties of CTAR1 in LMP1-Mediated Transformation J. Virol., September 15, 2007; 81(18): 9680 - 9692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dai, R. A. Phillips, and S. A. Ahmed Despite Inhibition of Nuclear Localization of NF-{kappa}B p65, c-Rel, and RelB, 17-beta Estradiol Up-Regulates NF-{kappa}B Signaling in Mouse Splenocytes: The Potential Role of Bcl-3 J. Immunol., August 1, 2007; 179(3): 1776 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. V. Yang, A. K. Sood, M. Chen, Y. Li, T. D. Eubank, C. B. Marsh, S. Jewell, N. A. Flavahan, C. Morrison, P.-E. Yeh, et al. Norepinephrine Up-regulates the Expression of Vascular Endothelial Growth Factor, Matrix Metalloproteinase (MMP)-2, and MMP-9 in Nasopharyngeal Carcinoma Tumor Cells Cancer Res., November 1, 2006; 66(21): 10357 - 10364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gao, D. L. Dai, M. Martinka, and G. Li Prognostic Significance of Nuclear Factor-{kappa}B p105/p50 in Human Melanoma and Its Role in Cell Migration. Cancer Res., September 1, 2006; 66(17): 8382 - 8388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Mainou and N. Raab-Traub LMP1 Strain Variants: Biological and Molecular Properties. J. Virol., July 1, 2006; 80(13): 6458 - 6468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kashatus, P. Cogswell, and A. S. Baldwin Expression of the Bcl-3 proto-oncogene suppresses p53 activation Genes & Dev., January 15, 2006; 20(2): 225 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Y. Yeh, S.-H. Kuo, K.-H. Yeh, S.-E. Chuang, C.-H. Hsu, W. C. Chang, H.-I Lin, M. Gao, and A.-L. Cheng A Pathway for Tumor Necrosis Factor-{alpha}-induced Bcl10 Nuclear Translocation: Bcl10 IS UP-REGULATED BY NF-{kappa}B AND PHOSPHORYLATED BY Akt1 AND THEN COMPLEXES WITH Bcl3 TO ENTER THE NUCLEUS J. Biol. Chem., January 6, 2006; 281(1): 167 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Serio, K. V. Reddy, and T. D. Bigby Lipopolysaccharide induces 5-lipoxygenase-activating protein gene expression in THP-1 cells via a NF-{kappa}B and C/EBP-mediated mechanism Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1125 - C1133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Guan, S. Hou, and R. P. Ricciardi DNA Binding of Repressor Nuclear Factor-{kappa}B p50/p50 Depends on Phosphorylation of Ser337 by the Protein Kinase A Catalytic Subunit J. Biol. Chem., March 18, 2005; 280(11): 9957 - 9962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Morrison, M. L. Gulley, R. Pathmanathan, and N. Raab-Traub Differential Signaling Pathways Are Activated in the Epstein-Barr Virus-Associated Malignancies Nasopharyngeal Carcinoma and Hodgkin Lymphoma Cancer Res., August 1, 2004; 64(15): 5251 - 5260. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
