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[Cancer Research 63, 2028-2032, May 1, 2003]
© 2003 American Association for Cancer Research

Advances in Brief

Molecular Characterization of Circulating EBV DNA in the Plasma of Nasopharyngeal Carcinoma and Lymphoma Patients1

K.C. Allen Chan2, Jun Zhang2, Anthony T.C. Chan, Kenny I.K. Lei, Sing-Fai Leung, Lisa Y.S. Chan, Katherine C.K. Chow and Y.M. Dennis Lo3

Departments of Chemical Pathology [K. C. A. C., J. Z., L. Y. S. C., K. C. K. C., Y. M. D. L.] and Clinical Oncology [A. T. C. C., K. I. K. L., S-F. L.], The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China

Despite the increasing clinical applications of circulating EBV DNA analysis as a tumor marker, the molecular nature of these EBV DNA molecules remains unclear. We subjected plasma/serum samples of nasopharyngeal carcinoma and lymphoma patients to DNase digestion and ultracentrifugation and showed that circulating EBV DNA molecules are "naked" DNA fragments instead of being contained inside virions. We further showed that these EBV DNA fragments were relatively short, and 87% of them were shorter than 181 bp. These results provide fundamental information that may improve our understanding of the release of tumor-derived nucleic acids into the blood of cancer patients.




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Copyright © 2003 by the American Association for Cancer Research.