This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Draffin, J. E. Articles by Waugh, D. J. J. Search for Related Content PubMed PubMed Citation Articles by Draffin, J. E. Articles by Waugh, D. J. J.

CD44 Potentiates the Adherence of Metastatic Prostate and Breast Cancer Cells to Bone Marrow Endothelial Cells

Department of Oncology, Centre for Cancer Research, Queen’s University Belfast, Belfast City Hospital, Belfast, Northern Ireland

The aim of this current study was to examine the significance of CD44 expression in mediating cancer cell adhesion to human bone marrow endothelial cell(s) (hBMEC). Differential CD44 expression on two metastatic prostate cancer cell lines, PC3 (CD44 +ve) and DU145 (CD44 –ve) and four breast cancer cell lines was confirmed by immunoblotting and immunocytochemistry. In cell adhesion assays, PC3 but not DU145 cells demonstrated a rapid adhesion to hBMECs. Treatment of PC3 cells with a neutralizing antibody against CD44 standard (CD44s) and CD44 splice variants decreased PC3 cell adhesion to hBMECs. Similarly, depletion of CD44 expression using RNA interference decreased the ability of PC3 cells and two CD44 +ve breast cancer cell lines (MDA-MB-231 and MDA-MB-157) to bind FITC-conjugated hyaluronan (FITC-HA) and to adhere to hBMECs. In contrast, transfection of DU145 cells or the T47D and MCF-7 breast cancer cell lines to express CD44s increased cell surface binding of FITC-HA and cell adherence to hBMECs. Treatment of PC3 and MDA-MD-231 cells but not hBMECs with hyaluronidase attenuated cell adhesion, suggesting that cell surface expression of CD44 on prostate and breast cancer cells may promote the retention of a HA coat that facilitates their initial arrest on bone marrow endothelium.

This article has been cited by other articles:

				S. R Barthel, J. D Gavino, G. K Wiese, J. M Jaynes, J. Siddiqui, and C. J Dimitroff Analysis of glycosyltransferase expression in metastatic prostate cancer cells capable of rolling activity on microvascular endothelial (E)-selectin Glycobiology, October 1, 2008; 18(10): 806 - 817. [Abstract] [Full Text] [PDF]

				C. Rampon, N. Weiss, C. Deboux, N. Chaverot, F. Miller, D. Buchet, H. Tricoire-Leignel, S. Cazaubon, A. Baron-Van Evercooren, and P.-O. Couraud Molecular Mechanism of Systemic Delivery of Neural Precursor Cells to the Brain: Assembly of Brain Endothelial Apical Cups and Control of Transmigration by CD44 Stem Cells, July 1, 2008; 26(7): 1673 - 1682. [Abstract] [Full Text] [PDF]

				Y.-X. Zhang, P. G. Knyazev, Y. V. Cheburkin, K. Sharma, Y. P. Knyazev, L. Orfi, I. Szabadkai, H. Daub, G. Keri, and A. Ullrich AXL Is a Potential Target for Therapeutic Intervention in Breast Cancer Progression Cancer Res., March 15, 2008; 68(6): 1905 - 1915. [Abstract] [Full Text] [PDF]

				C. D. McCabe, D. D. Spyropoulos, D. Martin, and C. S. Moreno Genome-Wide Analysis of the Homeobox C6 Transcriptional Network in Prostate Cancer Cancer Res., March 15, 2008; 68(6): 1988 - 1996. [Abstract] [Full Text] [PDF]

				A. J. Najy, K. C. Day, and M. L. Day ADAM15 Supports Prostate Cancer Metastasis by Modulating Tumor Cell-Endothelial Cell Interaction Cancer Res., February 15, 2008; 68(4): 1092 - 1099. [Abstract] [Full Text] [PDF]

				A. Ouhtit, Z. Y. Abd Elmageed, M. E. Abdraboh, T. F. Lioe, and M. H.G. Raj In Vivo Evidence for the Role of CD44s in Promoting Breast Cancer Metastasis to the Liver Am. J. Pathol., December 1, 2007; 171(6): 2033 - 2039. [Abstract] [Full Text] [PDF]

				A. Weigert, N. Tzieply, A. von Knethen, A. M. Johann, H. Schmidt, G. Geisslinger, and B. Brune Tumor Cell Apoptosis Polarizes Macrophages Role of Sphingosine-1-Phosphate Mol. Biol. Cell, October 1, 2007; 18(10): 3810 - 3819. [Abstract] [Full Text] [PDF]

				F.-Y. Liu and T.-C. Yen In Reply J. Clin. Oncol., April 1, 2007; 25(10): 1297 - 1299. [Full Text] [PDF]

				J. Wang, J. Wang, J. Dai, Y. Jung, C.-L. Wei, Y. Wang, A. M. Havens, P. J. Hogg, E. T. Keller, K. J. Pienta, et al. A Glycolytic Mechanism Regulating an Angiogenic Switch in Prostate Cancer Cancer Res., January 1, 2007; 67(1): 149 - 159. [Abstract] [Full Text] [PDF]

				M. S. Wicha Cancer stem cells and metastasis: lethal seeds. Clin. Cancer Res., October 1, 2006; 12(19): 5606 - 5607. [Full Text] [PDF]

				M. Balic, H. Lin, L. Young, D. Hawes, A. Giuliano, G. McNamara, R. H. Datar, and R. J. Cote Most early disseminated cancer cells detected in bone marrow of breast cancer patients have a putative breast cancer stem cell phenotype. Clin. Cancer Res., October 1, 2006; 12(19): 5615 - 5621. [Abstract] [Full Text] [PDF]

				A. C. Cook, A. F. Chambers, E. A. Turley, and A. B. Tuck Osteopontin Induction of Hyaluronan Synthase 2 Expression Promotes Breast Cancer Malignancy J. Biol. Chem., August 25, 2006; 281(34): 24381 - 24389. [Abstract] [Full Text] [PDF]

				C. M. van Golen, T. S. Schwab, B. Kim, M. E. Soules, S. Su Oh, K. Fung, K. L. van Golen, and E. L. Feldman Insulin-Like Growth Factor-I Receptor Expression Regulates Neuroblastoma Metastasis to Bone. Cancer Res., July 1, 2006; 66(13): 6570 - 6578. [Abstract] [Full Text] [PDF]

				H. Zhu, N. Mitsuhashi, A. Klein, L. W. Barsky, K. Weinberg, M. L. Barr, A. Demetriou, and G. D. Wu The Role of the Hyaluronan Receptor CD44 in Mesenchymal Stem Cell Migration in the Extracellular Matrix Stem Cells, April 1, 2006; 24(4): 928 - 935. [Abstract] [Full Text] [PDF]

				G M K Tse, P-H Tan, T K F Ma, C B Gilks, C S P Poon, and B K B Law CD44s is useful in the differentiation of benign and malignant papillary lesions of the breast J. Clin. Pathol., November 1, 2005; 58(11): 1185 - 1188. [Abstract] [Full Text] [PDF]

				C. J. Dimitroff, L. Descheny, N. Trujillo, R. Kim, V. Nguyen, W. Huang, K. J. Pienta, J. L. Kutok, and M. A. Rubin Identification of Leukocyte E-Selectin Ligands, P-Selectin Glycoprotein Ligand-1 and E-Selectin Ligand-1, on Human Metastatic Prostate Tumor Cells Cancer Res., July 1, 2005; 65(13): 5750 - 5760. [Abstract] [Full Text] [PDF]