Cancer Research Cell Death Mechanisms and Cancer Therapy Protein Translation and Cancer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nair, S. S.
Right arrow Articles by Vadlamudi, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nair, S. S.
Right arrow Articles by Vadlamudi, R. K.
[Cancer Research 64, 6416-6423, September 15, 2004]
© 2004 American Association for Cancer Research

Regular Articles

Potential Role of a Novel Transcriptional Coactivator PELP1 in Histone H1 Displacement in Cancer Cells

Sujit S. Nair, Sandip K. Mishra, Zhibo Yang, Seetharaman Balasenthil, Rakesh Kumar and Ratna K. Vadlamudi

Department of Molecular and Cellular Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas

The estrogen receptor plays an important role in breast cancer progression. Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), also called modulator of nongenomic activity of estrogen receptor (MNAR), a novel coactivator of estrogen receptor, modulates estrogen receptor transactivation functions. The mechanisms by which PELP1 modulates estrogen receptor genomic functions is not known. Here, using biochemical and scanning confocal microscopic analysis, we have demonstrated nuclear localization and functional implications of PELP1. Subnuclear fractionation showed PELP1 association with chromatin and nuclear matrix fractions. Ligand stimulation promoted recruitment of PELP1 to 17ß-estradiol responsive promoters, its colocalization with acetylated H3, and increased PELP1-associated histone acetyltransferase enzymatic activity. Far Western analysis revealed that PELP1 interacts with histone 1 and 3, with more preference toward histone 1. Using deletion analysis, we have identified the PELP1 COOH-terminal region as the histone 1 binding site. The PELP1 mutant lacking histone 1-binding domain acts as a dominant-negative and blocks estrogen receptor {alpha}-mediated transcription. Chromatin immunoprecipitation analysis showed a cyclic association and dissociation of PELP1 with the promoter, with recruitment of histone 1 and PELP1 occurring in opposite phases. PELP1 overexpression increased the micrococcal nuclease sensitivity of estrogen response element-containing nucleosomes. Our results provide novel insights about the transcription regulation of PELP1 and suggest that PELP1 participates in chromatin remodeling activity via displacement of histone 1 in cancer cells.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
V. M. Popov, J. Zhou, L. A. Shirley, J. Quong, W.-S. Yeow, J. A. Wright, K. Wu, H. Rui, R. K. Vadlamudi, J. Jiang, et al.
The Cell Fate Determination Factor DACH1 Is Expressed in Estrogen Receptor-{alpha}-Positive Breast Cancer and Represses Estrogen Receptor-{alpha} Signaling
Cancer Res., July 15, 2009; 69(14): 5752 - 5760.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. Kumar, H. Zhang, C. Holm, R. K. Vadlamudi, G. Landberg, and S. K. Rayala
Extranuclear Coactivator Signaling Confers Insensitivity to Tamoxifen
Clin. Cancer Res., June 15, 2009; 15(12): 4123 - 4130.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Kayahara, J. Ohanian, V. Ohanian, A. Berry, R. Vadlamudi, and D. W. Ray
MNAR functionally interacts with both NH2- and COOH-terminal GR domains to modulate transactivation
Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1047 - E1055.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Dimple, S. S. Nair, R. Rajhans, P. R. Pitcheswara, J. Liu, S. Balasenthil, X.-F. Le, M. E. Burow, N. Auersperg, R. R. Tekmal, et al.
Role of PELP1/MNAR Signaling in Ovarian Tumorigenesis
Cancer Res., June 15, 2008; 68(12): 4902 - 4909.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. K. Nagpal, S. Nair, D. Chakravarty, R. Rajhans, S. Pothana, D. W. Brann, R. R. Tekmal, and R. K. Vadlamudi
Growth Factor Regulation of Estrogen Receptor Coregulator PELP1 Functions via Protein Kinase A Pathway
Mol. Cancer Res., May 1, 2008; 6(5): 851 - 861.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. Rajhans, H. B. Nair, S. S. Nair, V. Cortez, K. Ikuko, N. B. Kirma, D. Zhou, A. E. Holden, D. W Brann, S. Chen, et al.
Modulation of in Situ Estrogen Synthesis by Proline-, Glutamic Acid-, and Leucine-Rich Protein-1: Potential Estrogen Receptor Autocrine Signaling Loop in Breast Cancer Cells
Mol. Endocrinol., March 1, 2008; 22(3): 649 - 664.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. E. Gururaj, S. Peng, R. K. Vadlamudi, and R. Kumar
Estrogen Induces Expression of BCAS3, a Novel Estrogen Receptor-{alpha} Coactivator, through Proline-, Glutamic Acid-, and Leucine-Rich Protein-1 (PELP1)
Mol. Endocrinol., August 1, 2007; 21(8): 1847 - 1860.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Rajhans, S. Nair, A. H. Holden, R. Kumar, R. R. Tekmal, and R. K. Vadlamudi
Oncogenic Potential of the Nuclear Receptor Coregulator Proline-, Glutamic Acid-, Leucine-Rich Protein 1/Modulator of the Nongenomic Actions of the Estrogen Receptor
Cancer Res., June 1, 2007; 67(11): 5505 - 5512.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. S. Nair, Z. Guo, J. M. Mueller, S. Koochekpour, Y. Qiu, R. R. Tekmal, R. Schule, H.-J. Kung, R. Kumar, and R. K. Vadlamudi
Proline-, Glutamic Acid-, and Leucine-Rich Protein-1/Modulator of Nongenomic Activity of Estrogen Receptor Enhances Androgen Receptor Functions through LIM-Only Coactivator, Four-and-a-Half LIM-Only Protein 2
Mol. Endocrinol., March 1, 2007; 21(3): 613 - 624.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. den Hollander, S. K. Rayala, D. Coverley, and R. Kumar
Ciz1, a Novel DNA-Binding Coactivator of the Estrogen Receptor {alpha}, Confers Hypersensitivity to Estrogen Action.
Cancer Res., November 15, 2006; 66(22): 11021 - 11029.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. Ohshiro, S. K. Rayala, M. D. Williams, R. Kumar, and A. K. El-Naggar
Biological Role of Estrogen Receptor {beta} in Salivary Gland Adenocarcinoma Cells.
Clin. Cancer Res., October 15, 2006; 12(20): 5994 - 5999.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Rayala, P. den Hollander, B. Manavathi, A. H. Talukder, C. Song, S. Peng, A. Barnekow, J. Kremerskothen, and R. Kumar
Essential Role of KIBRA in Co-activator Function of Dynein Light Chain 1 in Mammalian Cells
J. Biol. Chem., July 14, 2006; 281(28): 19092 - 19099.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Rosendorff, S. Sakakibara, S. Lu, E. Kieff, Y. Xuan, A. DiBacco, Y. Shi, Y. Shi, and G. Gill
NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression
PNAS, April 4, 2006; 103(14): 5308 - 5313.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Rayala, P. d. Hollander, S. Balasenthil, P. R. Molli, A. J. Bean, R. K. Vadlamudi, R.-A. Wang, and R. Kumar
Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (HRS) Interacts with PELP1 and Activates MAPK
J. Biol. Chem., February 17, 2006; 281(7): 4395 - 4403.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
S. K. Rayala, J. Mascarenhas, R. K. Vadlamudi, and R. Kumar
Altered localization of a coactivator sensitizes breast cancer cells to tumor necrosis factor-induced apoptosis.
Mol. Cancer Ther., February 1, 2006; 5(2): 230 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. E. Gururaj, S. K. Rayala, R. K. Vadlamudi, and R. Kumar
Novel Mechanisms of Resistance to Endocrine Therapy: Genomic and Nongenomic Considerations
Clin. Cancer Res., February 1, 2006; 12(3): 1001s - 1007s.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. K. Vadlamudi, B. Manavathi, S. Balasenthil, S. S. Nair, Z. Yang, A. A. Sahin, and R. Kumar
Functional Implications of Altered Subcellular Localization of PELP1 in Breast Cancer Cells
Cancer Res., September 1, 2005; 65(17): 7724 - 7732.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
B. Manavathi, S. S. Nair, R.-A. Wang, R. Kumar, and R. K. Vadlamudi
Proline-, Glutamic Acid-, and Leucine-Rich Protein-1 Is Essential in Growth Factor Regulation of Signal Transducers and Activators of Transcription 3 Activation
Cancer Res., July 1, 2005; 65(13): 5571 - 5577.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. K. Vadlamudi, S. Balasenthil, R. R. Broaddus, J.-A. Gustafsson, and R. Kumar
Deregulation of Estrogen Receptor Coactivator Proline-, Glutamic Acid-, and Leucine-Rich Protein-1/Modulator of Nongenomic Activity of Estrogen Receptor in Human Endometrial Tumors
J. Clin. Endocrinol. Metab., December 1, 2004; 89(12): 6130 - 6138.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2004 by the American Association for Cancer Research.