| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
1 Departments of Obstetrics and Gynecology,
2 Chemistry, and
3 Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan
Resveratrol (3,5,4-trihydroxystilbene), a natural phytoalexin present in grapes, nuts, and red wine, has antineoplastic activities. Several molecular mechanisms have been described to underlie its effects on cells in vitro and in vivo. In the present study, the response of ovarian cancer cells to resveratrol is explored. Resveratrol inhibited growth and induced death in a panel of five human ovarian carcinoma cell lines. The response was associated with mitochondrial release of cytochrome c, formation of the apoptosome complex, and caspase activation. Surprisingly, even with these molecular features of apoptosis, analysis of resveratrol-treated cells by light and electron microscopy revealed morphology and ultrastructural changes indicative of autophagocytic, rather than apoptotic, death. This suggests that resveratrol can induce cell death through two distinct pathways. Consistent with resveratrol’s ability to kill cells via nonapoptotic processes, cells transfected to express high levels of the antiapoptotic proteins Bcl-xL and Bcl-2 are equally sensitive as control cells to resveratrol. Together, these findings show that resveratrol induces cell death in ovarian cancer cells through a mechanism distinct from apoptosis, therefore suggesting that it may provide leverage to treat ovarian cancer that is chemoresistant on the basis of ineffective apoptosis.
This article has been cited by other articles:
|
|
 |
|
  K. Singletary and J. Milner Diet, Autophagy, and Cancer: A Review Cancer Epidemiol. Biomarkers Prev., July 1, 2008; 17(7): 1596 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. F. Trincheri, C. Follo, G. Nicotra, C. Peracchio, R. Castino, and C. Isidoro Resveratrol-induced apoptosis depends on the lipid kinase activity of Vps34 and on the formation of autophagolysosomes Carcinogenesis, February 1, 2008; 29(2): 381 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lefranc, V. Facchini, and R. Kiss Proautophagic Drugs: A Novel Means to Combat Apoptosis-Resistant Cancers, with a Special Emphasis on Glioblastomas Oncologist, December 1, 2007; 12(12): 1395 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohshiro, S. K. Rayala, S. Kondo, A. Gaur, R. K. Vadlamudi, A. K. El-Naggar, and R. Kumar Identifying the Estrogen Receptor Coactivator PELP1 in Autophagosomes Cancer Res., September 1, 2007; 67(17): 8164 - 8171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Gills, J. LoPiccolo, J. Tsurutani, R. H. Shoemaker, C. J.M. Best, M. S. Abu-Asab, J. Borojerdi, N. A. Warfel, E. R. Gardner, M. Danish, et al. Nelfinavir, A Lead HIV Protease Inhibitor, Is a Broad-Spectrum, Anticancer Agent that Induces Endoplasmic Reticulum Stress, Autophagy, and Apoptosis In vitro and In vivo Clin. Cancer Res., September 1, 2007; 13(17): 5183 - 5194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. F. Trincheri, G. Nicotra, C. Follo, R. Castino, and C. Isidoro Resveratrol induces cell death in colorectal cancer cells by a novel pathway involving lysosomal cathepsin D Carcinogenesis, May 1, 2007; 28(5): 922 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chaudhuri, S. Orsulic, and B. T. Ashok Antiproliferative activity of sulforaphane in Akt-overexpressing ovarian cancer cells Mol. Cancer Ther., January 1, 2007; 6(1): 334 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Ellington, M. A. Berhow, and K. W. Singletary Inhibition of Akt signaling and enhanced ERK1/2 activity are involved in induction of macroautophagy by triterpenoid B-group soyasaponins in colon cancer cells Carcinogenesis, February 1, 2006; 27(2): 298 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tyagi, R. P. Singh, C. Agarwal, S. Siriwardana, R. A. Sclafani, and R. Agarwal Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells Carcinogenesis, November 1, 2005; 26(11): 1978 - 1987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-A. Gonzalez-Polo, P. Boya, A.-L. Pauleau, A. Jalil, N. Larochette, S. Souquere, E.-L. Eskelinen, G. Pierron, P. Saftig, and G. Kroemer The apoptosis/autophagy paradox: autophagic vacuolization before apoptotic death J. Cell Sci., July 15, 2005; 118(14): 3091 - 3102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Boya, R.-A. Gonzalez-Polo, N. Casares, J.-L. Perfettini, P. Dessen, N. Larochette, D. Metivier, D. Meley, S. Souquere, T. Yoshimori, et al. Inhibition of Macroautophagy Triggers Apoptosis Mol. Cell. Biol., February 1, 2005; 25(3): 1025 - 1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Ellington, M. Berhow, and K. W. Singletary Induction of macroautophagy in human colon cancer cells by soybean B-group triterpenoid saponins Carcinogenesis, January 1, 2005; 26(1): 159 - 167. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
