Cancer Research Meeting Calendar Genetics and Biology of Brain Cancer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Onken, M. D.
Right arrow Articles by Harbour, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Onken, M. D.
Right arrow Articles by Harbour, J. W.
[Cancer Research 64, 7205-7209, October 15, 2004]
© 2004 American Association for Cancer Research

Advances in Brief

Gene Expression Profiling in Uveal Melanoma Reveals Two Molecular Classes and Predicts Metastatic Death

Michael D. Onken, Lori A. Worley, Justis P. Ehlers and J. William Harbour

Department of Ophthalmology & Visual Sciences, Washington University School of Medicine, St. Louis, Missouri

Melanomas are notoriously difficult to classify because of a lack of discrete clinical and pathological stages. Here, we show that primary uveal melanomas surprisingly cluster into two distinct molecular classes based on gene expression profile. Genes that discriminate class 1 (low-grade) from class 2 (high-grade) include highly significant clusters of down-regulated genes on chromosome 3 and up-regulated genes on chromosome 8q, which is consistent with previous cytogenetic studies. A three-gene signature allows biopsy-size tumor samples to be assigned accurately to tumor classes using either array or PCR platforms. Most importantly, this molecular classification strongly predicts metastatic death and outperforms other clinical and pathological prognostic indicators. These studies offer new insights into melanoma pathogenesis, and they provide a practical foundation for effective clinical predictive testing.




This article has been cited by other articles:


Home page
 IOVSHome page
J. Trolet, P. Hupe, I. Huon, I. Lebigot, C. Decraene, O. Delattre, X. Sastre-Garau, S. Saule, J.-P. Thiery, C. Plancher, et al.
Genomic Profiling and Identification of High-Risk Uveal Melanoma by Array CGH Analysis of Primary Tumors and Liver Metastases
Invest. Ophthalmol. Vis. Sci., June 1, 2009; 50(6): 2572 - 2580.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
B. Damato and S. E. Coupland
Translating Uveal Melanoma Cytogenetics Into Clinical Care
Arch Ophthalmol, April 1, 2009; 127(4): 423 - 429.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. D. Onken, L. A. Worley, M. D. Long, S. Duan, M. L. Council, A. M. Bowcock, and J. W. Harbour
Oncogenic Mutations in GNAQ Occur Early in Uveal Melanoma
Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5230 - 5234.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. van Gils, E. M. Lodder, H. W. Mensink, E. Kilic, N. C. Naus, H. T. Bruggenwirth, W. van IJcken, D. Paridaens, G. P. Luyten, and A. de Klein
Gene Expression Profiling in Uveal Melanoma: Two Regions on 3p Related to Prognosis
Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4254 - 4262.
[Abstract] [Full Text] [PDF]

Home page
 Mayo Clin Proc.Home page
A. Sekulic, P. Haluska Jr, A. J. Miller, J. G. De Lamo, S. Ejadi, J. S. Pulido, D. R. Salomao, E. C. Thorland, R. G. Vile, D. L. Swanson, et al.
Malignant Melanoma in the 21st Century: The Emerging Molecular Landscape
Mayo Clin. Proc., July 1, 2008; 83(7): 825 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. D. Onken, L. A. Worley, and J. W. Harbour
A Metastasis Modifier Locus on Human Chromosome 8p in Uveal Melanoma Identified by Integrative Genomic Analysis
Clin. Cancer Res., June 15, 2008; 14(12): 3737 - 3745.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
T. Tsai and J. M. O'Brien
The Future Promise and the Current Reality of Genetic Prognostication in Patients With Uveal Melanoma
Arch Ophthalmol, March 1, 2008; 126(3): 413 - 415.
[Full Text] [PDF]

Home page
 Arch OphthalmolHome page
J. A. Shields, C. L. Shields, M. Materin, T. Sato, and A. Ganguly
Role of Cytogenetics in Management of Uveal Melanoma
Arch Ophthalmol, March 1, 2008; 126(3): 416 - 419.
[Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
S. Bakalian, J.-C. Marshall, P. Logan, D. Faingold, S. Maloney, S. Di Cesare, C. Martins, B. F. Fernandes, and M. N. Burnier Jr.
Molecular Pathways Mediating Liver Metastasis in Patients with Uveal Melanoma
Clin. Cancer Res., February 15, 2008; 14(4): 951 - 956.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Maat, L. V. Ly, E. S. Jordanova, D. de Wolff-Rouendaal, N. E. Schalij-Delfos, and M. J. Jager
Monosomy of Chromosome 3 and an Inflammatory Phenotype Occur Together in Uveal Melanoma
Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 505 - 510.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. P. Ehlers, L. Worley, M. D. Onken, and J. W. Harbour
Integrative Genomic Analysis of Aneuploidy in Uveal Melanoma
Clin. Cancer Res., January 1, 2008; 14(1): 115 - 122.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Meir, R. Dror, X. Yu, J. Qian, I. Simon, J. Pe'er, and I. Chowers
Molecular Characteristics of Liver Metastases from Uveal Melanoma
Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 4890 - 4896.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
J. R. Latendresse, L. Muskhelishvili, A. Warbritton, and W. H. Tolleson
Two Cases of Uveal Amelanotic Melanoma in Transgenic Tyr-HRAS+ Ink4a/Arf Heterozygous Mice
Toxicol Pathol, October 1, 2007; 35(6): 825 - 830.
[Abstract] [Full Text] [PDF]

Home page
 Br J OphthalmolHome page
A. D Singh, K. Sisley, Y. Xu, J. Li, P. Faber, S. J Plummer, H. S Mudhar, I. G Rennie, P. M Kessler, G. Casey, et al.
Reduced expression of autotaxin predicts survival in uveal melanoma
Br J Ophthalmol, October 1, 2007; 91(10): 1385 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Loudig, E. Milova, M. Brandwein-Gensler, A. Massimi, T. J. Belbin, G. Childs, R. H. Singer, T. Rohan, and M. B. Prystowsky
Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)
Nucleic Acids Res., August 1, 2007; 35(15): e94 - e94.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
C. L. Shields, A. Ganguly, M. A. Materin, L. Teixeira, A. Mashayekhi, L. A. Swanson, B. P. Marr, and J. A. Shields
Chromosome 3 Analysis of Uveal Melanoma Using Fine-Needle Aspiration Biopsy at the Time of Plaque Radiotherapy in 140 Consecutive Cases: The Deborah Iverson, MD, Lectureship
Arch Ophthalmol, August 1, 2007; 125(8): 1017 - 1024.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
J. W. Harbour
Molecular Prognostic Testing in Uveal Melanoma: Has It Finally Come of Age?
Arch Ophthalmol, August 1, 2007; 125(8): 1122 - 1123.
[Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. D. Onken, L. A. Worley, E. Person, D. H. Char, A. M. Bowcock, and J. W. Harbour
Loss of Heterozygosity of Chromosome 3 Detected with Single Nucleotide Polymorphisms Is Superior to Monosomy 3 for Predicting Metastasis in Uveal Melanoma
Clin. Cancer Res., May 15, 2007; 13(10): 2923 - 2927.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. J. Bosch, J. A. Thompson, M. K. Srivastava, U. K. Iheagwara, T. G. Murray, M. Lotem, B. R. Ksander, and S. Ostrand-Rosenberg
MHC Class II-Transduced Tumor Cells Originating in the Immune-Privileged Eye Prime and Boost CD4+ T Lymphocytes that Cross-react with Primary and Metastatic Uveal Melanoma Cells
Cancer Res., May 1, 2007; 67(9): 4499 - 4506.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. A. Worley, M. D. Onken, E. Person, D. Robirds, J. Branson, D. H. Char, A. Perry, and J. W. Harbour
Transcriptomic versus Chromosomal Prognostic Markers and Clinical Outcome in Uveal Melanoma
Clin. Cancer Res., March 1, 2007; 13(5): 1466 - 1471.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Sandinha, M. Farquharson, I. McKay, and F. Roberts
Correlation of Heterogeneity for Chromosome 3 Copy Number with Cell Type in Choroidal Melanoma of Mixed-Cell Type
Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5177 - 5180.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
P Kiewe, N. Bechrakis, A Schmittel, P Ruf, H Lindhofer, E Thiel, and D Nagorsen
Increased chondroitin sulphate proteoglycan expression (B5 immunoreactivity) in metastases of uveal melanoma
Ann. Onc., December 1, 2006; 17(12): 1830 - 1834.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
M. D. Onken, L. A. Worley, R. M. Davila, D. H. Char, and J. W. Harbour
Prognostic Testing in Uveal Melanoma by Transcriptomic Profiling of Fine Needle Biopsy Specimens
J. Mol. Diagn., November 1, 2006; 8(5): 567 - 573.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. S. Scicchitano, D. A. Dalmas, M. A. Bertiaux, S. M. Anderson, L. R. Turner, R. A. Thomas, R. Mirable, and R. W. Boyce
Preliminary Comparison of Quantity, Quality, and Microarray Performance of RNA Extracted From Formalin-fixed, Paraffin-embedded, and Unfixed Frozen Tissue Samples
J. Histochem. Cytochem., November 1, 2006; 54(11): 1229 - 1237.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. Chin, L. A. Garraway, and D. E. Fisher
Malignant melanoma: genetics and therapeutics in the genomic era.
Genes & Dev., August 15, 2006; 20(16): 2149 - 2182.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Q. Wang, S. Diskin, E. Rappaport, E. Attiyeh, Y. Mosse, D. Shue, E. Seiser, J. Jagannathan, S. Shusterman, M. Bansal, et al.
Integrative genomics identifies distinct molecular classes of neuroblastoma and shows that multiple genes are targeted by regional alterations in DNA copy number.
Cancer Res., June 15, 2006; 66(12): 6050 - 6062.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. W. Harbour
Eye Cancer: Unique Insights into Oncogenesis The Cogan Lecture
Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1737 - 1745.
[Full Text] [PDF]

Home page
 Cancer Res.Home page
M. D. Onken, J. P. Ehlers, L. A. Worley, J. Makita, Y. Yokota, and J. W. Harbour
Functional Gene Expression Analysis Uncovers Phenotypic Switch in Aggressive Uveal Melanomas.
Cancer Res., May 1, 2006; 66(9): 4602 - 4609.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
S. Peters, V. Voelter, L. Zografos, S. Pampallona, R. Popescu, M. Gillet, W. Bosshard, G. Fiorentini, M. Lotem, R. Weitzen, et al.
Intra-arterial hepatic fotemustine for the treatment of liver metastases from uveal melanoma: experience in 101 patients
Ann. Onc., April 1, 2006; 17(4): 578 - 583.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Zuidervaart, P. J. Hensbergen, M.-C. Wong, A. M. Deelder, C. P. Tensen, M. J. Jager, and N. A. Gruis
Proteomic analysis of uveal melanoma reveals novel potential markers involved in tumor progression.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 786 - 793.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. S. Kadkol, A. Y. Lin, V. Barak, I. Kalickman, L. Leach, K. Valyi-Nagy, D. Majumdar, S. Setty, A. J. Maniotis, R. Folberg, et al.
Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 802 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Elfilali, S. Lair, C. Verbeke, P. La Rosa, F. Radvanyi, and E. Barillot
ITTACA: a new database for integrated tumor transcriptome array and clinical data analysis
Nucleic Acids Res., January 1, 2006; 34(suppl_1): D613 - D616.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
P. Rietschel, K. S. Panageas, C. Hanlon, A. Patel, D. H. Abramson, and P. B. Chapman
Variates of Survival in Metastatic Uveal Melanoma
J. Clin. Oncol., November 1, 2005; 23(31): 8076 - 8080.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. P. Ehlers, L. Worley, M. D. Onken, and J. W. Harbour
DDEF1 Is Located in an Amplified Region of Chromosome 8q and Is Overexpressed in Uveal Melanoma
Clin. Cancer Res., May 15, 2005; 11(10): 3609 - 3613.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. P. Ehlers and J. W. Harbour
NBS1 Expression as a Prognostic Marker in Uveal Melanoma
Clin. Cancer Res., March 1, 2005; 11(5): 1849 - 1853.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2004 by the American Association for Cancer Research.