Cancer Research 09 AM Call for Abstracts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mishra, S.
Right arrow Articles by Murphy, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mishra, S.
Right arrow Articles by Murphy, L. J.
[Cancer Research 64, 2516-2522, April 1, 2004]
© 2004 American Association for Cancer Research

Regular Articles

Insulin-Like Growth Factor Binding Protein-3 Interacts with Autocrine Motility Factor/Phosphoglucose Isomerase (AMF/PGI) and Inhibits the AMF/PGI Function

Suresh Mishra1, Avrahram Raz3 and Liam J. Murphy1,2

Departments of 1 Physiology and 2 Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada, and 3 Barbara Ann Karmanos Cancer Institute, Wayne State University, School of Medicine, Detroit, Michigan

Autocrine motility factor/phosphoglucose isomerase (AMF/PGI) was identified as a binding partner for insulin-like growth factor binding protein-3 (IGFBP-3) in solubilized T47D and MCF-7 human breast cancer cell membranes. The interaction between AMF/PGI and IGFBP-3 was verified by cross-linking biotinylated IGFBP-3 to intact cells. After solubilization of the membranes, the biotinylated complexes were precipitated with streptavidin-agarose conjugate and analyzed by SDS-PAGE. A Mr ~80,000 complex was identified when the nitrocellulose membranes were probed either with streptavidin-horseradish peroxidase conjugate or AMF/PGI antiserum confirming the cross-linking of IGFBP-3 to AMF/PGI. The interaction between IGFBP-3 and AMF/PGI was also further confirmed by ligand blotting of purified AMF/PGI using biotinylated IGFBP-3. Both glycosylated and nonglycosylated IGFBP-3 inhibited the catalytic activity of AMF/PGI in a dose-dependent fashion. In addition, IGFBP-3 inhibited the binding of AMF/PGI to breast cancer cells and AMF/PGI-induced migration of both T47D and MCF-7 human breast cancer cells. IGFBP-3 also decreased the phosphorylation of AMF/PGI and reduced the translocation of AMF/PGI to the cell membrane and AMF/PGI. AMF/PGI resulted in a dose-dependent inhibition of IGFBP-3 induced apoptosis in T47D and MCF-7 cells. In summary, we have identified AMF/PGI as a membrane-associated binding partner for IGFBP-3 in breast cancer cells. The ability of IGFBP-3 to bind and inhibit the actions of AMF/PGI may have some role in the antiproliferative proapoptotic effects of IGFBP-3.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
T. Yanagawa, T. Funasaka, S. Tsutsumi, H. Hu, H. Watanabe, and A. Raz
Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Activities by the Poly(ADP-Ribose) Polymerase Family-14
Cancer Res., September 15, 2007; 67(18): 8682 - 8689.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Oufattole, S. W.-J. Lin, B. Liu, D. Mascarenhas, P. Cohen, and B. D. Rodgers
Ribonucleic Acid Polymerase II Binding Subunit 3 (Rpb3), a Potential Nuclear Target of Insulin-Like Growth Factor Binding Protein-3
Endocrinology, May 1, 2006; 147(5): 2138 - 2146.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. V. Silha, P. C. Sheppard, S. Mishra, Y. Gui, J. Schwartz, J. G. Dodd, and L. J. Murphy
Insulin-Like Growth Factor (IGF) Binding Protein-3 Attenuates Prostate Tumor Growth by IGF-Dependent and IGF-Independent Mechanisms
Endocrinology, May 1, 2006; 147(5): 2112 - 2121.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Al-Zahrani, M. S. Sandhu, R. N. Luben, D. Thompson, C. Baynes, K. A. Pooley, C. Luccarini, H. Munday, B. Perkins, P. Smith, et al.
IGF1 and IGFBP3 tagging polymorphisms are associated with circulating levels of IGF1, IGFBP3 and risk of breast cancer
Hum. Mol. Genet., January 1, 2006; 15(1): 1 - 10.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Lagana, J. G. Goetz, N. Y, Y. Altschuler, and I. R. Nabi
pH-specific sequestration of phosphoglucose isomerase/autocrine motility factor by fibronectin and heparan sulphate
J. Cell Sci., September 15, 2005; 118(18): 4175 - 4185.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
B. Liu, K.-W. Lee, H. Li, L. Ma, G. L. Lin, R. A.S. Chandraratna, and P. Cohen
Combination Therapy of Insulin-Like Growth Factor Binding Protein-3 and Retinoid X Receptor Ligands Synergize on Prostate Cancer Cell Apoptosis In vitro and In vivo
Clin. Cancer Res., July 1, 2005; 11(13): 4851 - 4856.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
J. T. Prchal and X. T. Gregg
Red Cell Enzymes
Hematology, January 1, 2005; 2005(1): 19 - 23.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2004 by the American Association for Cancer Research.