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Acetylcholinesterase Plays a Pivotal Role in Apoptosome Formation

¹ Department of Anatomy and Cell Biology (BK21 program), Dong-A University College of Medicine and Medical Science Research Center, Seo Gu, Busan, South Korea, and ² Department of Pharmacy, College of Pharmacy, Pusan National University, Geumjeong-gu, Busan, South Korea

Although a recent study (Zhang et al. Cell Death Differ 2002; 9; 790–800) presented that acetylcholinesterase (AChE) might be an important common component in leading to various types of apoptosis, the molecular mechanism, by which AChE functions, had remained elusive before that study. We explored the role of AChE in apoptosis by silencing the AChE gene. Silencing of the AChE gene abolished the expression of AChE and prevented caspase-9 activation, decrease of cell viability, nuclear condensation and poly(adenosine diphosphate-ribose) polymerase cleavage but not mitochondrial events. Importantly, silencing of the AChE gene blocked the interaction between apoptotic protease-activating factor-1 and cytochrome c. Here we propose that AChE plays a pivotal role in the formation of apoptosome.

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