Cancer Research Infection and Cancer: Biology, Therapeutics, and Prevention Translational Medicine Conference in Israel
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quaglino, E.
Right arrow Articles by Cavallo, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quaglino, E.
Right arrow Articles by Cavallo, F.
[Cancer Research 64, 2858-2864, April 15, 2004]
© 2004 American Association for Cancer Research

Immunology

Electroporated DNA Vaccine Clears Away Multifocal Mammary Carcinomas in Her-2/neu Transgenic Mice

Elena Quaglino1, Manuela Iezzi2, Cristina Mastini1, Augusto Amici3, Federica Pericle1, Emma Di Carlo2, Serenella M. Pupa4, Carla De Giovanni5, Michela Spadaro1, Claudia Curcio1, Pier Luigi Lollini5, Piero Musiani2, Guido Forni1 and Federica Cavallo1

1 Department of Clinical and Biological Sciences, University of Turin, Orbassano; 2 Center of Excellence on Aging (CeSI), University of Chieti; 3 Department of Molecular, Cellular and Animal Biology, University of Camerino, Camerino; 4 Molecular Targeting Unit, National Cancer Institute, Milan; and 5 Cancer Research Section, Department of Experimental Pathology, University of Bologna, Bologna, Italy

The transforming rat Her-2/neu oncogene embedded into the genome of virgin transgenic BALB/c mice (BALB-neuT) provokes the development of an invasive carcinoma in each of their 10 mammary glands. i.m. vaccination with DNA plasmids coding for the extracellular and transmembrane domains of the protein product of the Her-2/neu oncogene started when mice already display multifocal in situ carcinomas temporarily halts neoplastic progression, but all mice develop a tumor by week 43. By contrast, progressive clearance of neoplastic lesions and complete protection of all 1-year-old mice are achieved when the same plasmids are electroporated at 10-week intervals. Pathological findings, in vitro tests, and the results from the immunization of both IFN-{gamma} and immunoglobulin gene knockout BALB-neuT mice, and of adoptive transfer experiments, all suggest that tumor clearance rests on the combination of antibodies and IFN-{gamma}-releasing T cells. These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions.




This article has been cited by other articles:


Home page
 J. Virol.Home page
A. Luckay, M. K. Sidhu, R. Kjeken, S. Megati, S.-Y. Chong, V. Roopchand, D. Garcia-Hand, R. Abdullah, R. Braun, D. C. Montefiori, et al.
Effect of Plasmid DNA Vaccine Design and In Vivo Electroporation on the Resulting Vaccine-Specific Immune Responses in Rhesus Macaques
J. Virol., May 15, 2007; 81(10): 5257 - 5269.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. Nava-Parada, G. Forni, K. L. Knutson, L. R. Pease, and E. Celis
Peptide Vaccine Given with a Toll-Like Receptor Agonist Is Effective for the Treatment and Prevention of Spontaneous Breast Tumors
Cancer Res., February 1, 2007; 67(3): 1326 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Rolla, C. Nicolo, S. Malinarich, M. Orsini, G. Forni, F. Cavallo, and F. Ria
Distinct and Non-Overlapping T Cell Receptor Repertoires Expanded by DNA Vaccination in Wild-Type and HER-2 Transgenic BALB/c Mice
J. Immunol., December 1, 2006; 177(11): 7626 - 7633.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. Ambrosino, M. Spadaro, M. Iezzi, C. Curcio, G. Forni, P. Musiani, W.-Z. Wei, and F. Cavallo
Immunosurveillance of erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance.
Cancer Res., August 1, 2006; 66(15): 7734 - 7740.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Pannellini, M. Spadaro, E. Di Carlo, E. Ambrosino, M. Iezzi, A. Amici, P. L. Lollini, G. Forni, F. Cavallo, and P. Musiani
Timely DNA Vaccine Combined with Systemic IL-12 Prevents Parotid Carcinomas before a Dominant-Negative p53 Makes Their Growth Independent of HER-2/neu Expression.
J. Immunol., June 15, 2006; 176(12): 7695 - 7703.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Holmgren, E. Ambrosino, O. Birot, C. Tullus, N. Veitonmaki, T. Levchenko, L.-M. Carlson, P. Musiani, M. Iezzi, C. Curcio, et al.
A DNA vaccine targeting angiomotin inhibits angiogenesis and suppresses tumor growth
PNAS, June 13, 2006; 103(24): 9208 - 9213.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Tegerstedt, J. A. Lindencrona, C. Curcio, K. Andreasson, C. Tullus, G. Forni, T. Dalianis, R. Kiessling, and T. Ramqvist
A Single Vaccination with Polyomavirus VP1/VP2Her2 Virus-Like Particles Prevents Outgrowth of HER-2/neu-Expressing Tumors
Cancer Res., July 1, 2005; 65(13): 5953 - 5957.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Park, M. Terabe, Y. Sakai, J. Munasinghe, G. Forni, J. C. Morris, and J. A. Berzofsky
Early Role of CD4+ Th1 Cells and Antibodies in HER-2 Adenovirus Vaccine Protection against Autochthonous Mammary Carcinomas
J. Immunol., April 1, 2005; 174(7): 4228 - 4236.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Spadaro, E. Ambrosino, M. Iezzi, E. Di Carlo, P. Sacchetti, C. Curcio, A. Amici, W.-Z. Wei, P. Musiani, P.-L. Lollini, et al.
Cure of Mammary Carcinomas in Her-2 Transgenic Mice through Sequential Stimulation of Innate (Neoadjuvant Interleukin-12) and Adaptive (DNA Vaccine Electroporation) Immunity
Clin. Cancer Res., March 1, 2005; 11(5): 1941 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. M. Pupa, M. Iezzi, E. Di Carlo, A. Invernizzi, F. Cavallo, R. Meazza, A. Comes, S. Ferrini, P. Musiani, and S. Menard
Inhibition of Mammary Carcinoma Development in HER-2/neu Transgenic Mice through Induction of Autoimmunity by Xenogeneic DNA Vaccination
Cancer Res., February 1, 2005; 65(3): 1071 - 1078.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Croci, G. Nicoletti, L. Landuzzi, C. De Giovanni, A. Astolfi, C. Marini, E. Di Carlo, P. Musiani, G. Forni, P. Nanni, et al.
Immunological Prevention of a Multigene Cancer Syndrome
Cancer Res., November 15, 2004; 64(22): 8428 - 8434.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Takeuchi, S.-i. Hiraoka, X.-Y. Zhou, M. Nagafuku, S. Ono, T. Tsujimura, M. Nakazawa, Y. Yura, T. Hamaoka, and H. Fujiwara
Anti-HER-2/neu Immune Responses Are Induced before the Development of Clinical Tumors but Declined following Tumorigenesis in HER-2/neu Transgenic Mice
Cancer Res., October 15, 2004; 64(20): 7588 - 7595.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Nanni, L. Landuzzi, G. Nicoletti, C. De Giovanni, I. Rossi, S. Croci, A. Astolfi, M. Iezzi, E. Di Carlo, P. Musiani, et al.
Immunoprevention of Mammary Carcinoma in HER-2/neu Transgenic Mice Is IFN-{gamma} and B Cell Dependent
J. Immunol., August 15, 2004; 173(4): 2288 - 2296.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2004 by the American Association for Cancer Research.