This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lillard-Wetherell, K. Articles by Groden, J. Search for Related Content PubMed PubMed Citation Articles by Lillard-Wetherell, K. Articles by Groden, J.

BLM Helicase Complements Disrupted Type II Telomere Lengthening in Telomerase-Negative sgs1 Yeast

Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio

Requests for reprints: Joanna Groden, Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524. Phone: 513-558-0088; Fax: 513-558-2794; E-mail: seelkat{at}sbcglobal.net.

Recombination-mediated pathways for telomere lengthening may be utilized in the absence of telomerase activity. The RecQ-like helicases, BLM and Sgs1, are implicated in recombination-mediated telomere lengthening in human cells and budding yeast, respectively. Here, we show that BLM expression rescues disrupted telomere lengthening in telomerase-negative sgs1 yeast. BLM helicase activity is required for this complementation, indicating BLM and Sgs1 resolve the same telomeric structures. These data support a conserved function for BLM and Sgs1 in recombination-mediated telomere lengthening.

This article has been cited by other articles:

				S. Bhattacharyya, J. Keirsey, B. Russell, J. Kavecansky, K. Lillard-Wetherell, K. Tahmaseb, J. J. Turchi, and J. Groden Telomerase-associated Protein 1, HSP90, and Topoisomerase II{alpha} Associate Directly with the BLM Helicase in Immortalized Cells Using ALT and Modulate Its Helicase Activity Using Telomeric DNA Substrates J. Biol. Chem., May 29, 2009; 284(22): 14966 - 14977. [Abstract] [Full Text] [PDF]

				J. Y. Lee, J. L. Mogen, A. Chavez, and F. B. Johnson Sgs1 RecQ Helicase Inhibits Survival of Saccharomyces cerevisiae Cells Lacking Telomerase and Homologous Recombination J. Biol. Chem., October 31, 2008; 283(44): 29847 - 29858. [Abstract] [Full Text] [PDF]

				R. M. Brosh Jr and V. A. Bohr Human premature aging, DNA repair and RecQ helicases Nucleic Acids Res., December 3, 2007; 35(22): 7527 - 7544. [Abstract] [Full Text] [PDF]