This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tantin, D. Articles by Sharp, P. A. Search for Related Content PubMed PubMed Citation Articles by Tantin, D. Articles by Sharp, P. A.

The Octamer Binding Transcription Factor Oct-1 Is a Stress Sensor

¹ Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts and Departments of ² Medicine and ³ Biochemistry, Microbiology, and Immunology, University of Ottawa, The Ottawa Health Science Research Institute, Ottawa, Ontario, Canada

Requests for reprints: Phillip A. Sharp, Center for Cancer Research, Massachusetts Institute of Technology, Room E17-529, 77 Massachusetts Avenue, Cambridge, MA 02139-4307. Phone: 617-253-6421; Fax: 617-253-3867; E-mail: sharppa{at}mit.edu.

The POU-domain transcription factor Oct-1 is widely expressed in adult tissues and has been proposed to regulate a large group of target genes. Microarray expression profiling was used to evaluate gene expression changes in Oct-1-deficient mouse fibroblasts. A number of genes associated with cellular stress exhibited altered expression. Consistent with this finding, Oct-1-deficient fibroblasts were hypersensitive to radiation, doxorubicin, and hydrogen peroxide and harbored elevated reactive oxygen species. Expression profiling identified a second group of genes dysregulated in Oct-1-deficient fibroblasts following irradiation, including many associated with oxidative and metabolic stress. A number of these genes contain octamer sequences in their immediate 5' regulatory regions, some of which are conserved in human. These results indicate that Oct-1 modulates the activity of genes important for the cellular response to stress.

This article has been cited by other articles:

				J. Lu, S. Zhang, D. Chen, H. Wang, W. Wu, X. Wang, Y. Lei, J. Wang, J. Qian, W. Fan, et al. Functional characterization of a promoter polymorphism in APE1/Ref-1 that contributes to reduced lung cancer susceptibility FASEB J, October 1, 2009; 23(10): 3459 - 3469. [Abstract] [Full Text] [PDF]

				P. Wang, Q. Wang, J. Sun, J. Wu, H. Li, N. Zhang, Y. Huang, B. Su, R.-k. Li, L. Liu, et al. POU Homeodomain Protein Oct-1 Functions as a Sensor for Cyclic AMP J. Biol. Chem., September 25, 2009; 284(39): 26456 - 26465. [Abstract] [Full Text] [PDF]

				A. S. Sirek, L. Liu, M. Naples, K. Adeli, D. S. Ng, and T. Jin Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, and Unc-86 Homeodomain Protein Octamer Transcription Factor-1 Endocrinology, August 1, 2009; 150(8): 3483 - 3492. [Abstract] [Full Text] [PDF]

				E. Theodorou, G. Dalembert, C. Heffelfinger, E. White, S. Weissman, L. Corcoran, and M. Snyder A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation Genes & Dev., March 1, 2009; 23(5): 575 - 588. [Abstract] [Full Text] [PDF]

				J. Kang, M. Gemberling, M. Nakamura, F. G. Whitby, H. Handa, W. G. Fairbrother, and D. Tantin A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress Genes & Dev., January 15, 2009; 23(2): 208 - 222. [Abstract] [Full Text] [PDF]

				A. N. Malhas, C. F. Lee, and D. J. Vaux Lamin B1 controls oxidative stress responses via Oct-1 J. Cell Biol., January 12, 2009; 184(1): 45 - 55. [Abstract] [Full Text] [PDF]

				R. C. Fry, J. P. Svensson, C. Valiathan, E. Wang, B. J. Hogan, S. Bhattacharya, J. M. Bugni, C. A. Whittaker, and L. D. Samson Genomic predictors of interindividual differences in response to DNA damaging agents Genes & Dev., October 1, 2008; 22(19): 2621 - 2626. [Abstract] [Full Text] [PDF]

				C. Zhou, Y. Tong, K. Wawrowsky, S. Bannykh, I. Donangelo, and S. Melmed Oct-1 induces pituitary tumor transforming gene expression in endocrine tumors Endocr. Relat. Cancer, September 1, 2008; 15(3): 817 - 831. [Abstract] [Full Text] [PDF]

				T. Thum and J. Borlak LOX-1 Receptor Blockade Abrogates oxLDL-induced Oxidative DNA Damage and Prevents Activation of the Transcriptional Repressor Oct-1 in Human Coronary Arterial Endothelium J. Biol. Chem., July 11, 2008; 283(28): 19456 - 19464. [Abstract] [Full Text] [PDF]

				G. Qiu and J. S. Hill Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages J. Lipid Res., October 1, 2007; 48(10): 2112 - 2122. [Abstract] [Full Text] [PDF]

				N. G. d. Paz, S. Simeonidis, C. Leo, D. W. Rose, and T. Collins Regulation of NF-{kappa}B-dependent Gene Expression by the POU Domain Transcription Factor Oct-1 J. Biol. Chem., March 16, 2007; 282(11): 8424 - 8434. [Abstract] [Full Text] [PDF]

				F. Shen, Z. Hu, J. Goswami, and S. L. Gaffen Identification of Common Transcriptional Regulatory Elements in Interleukin-17 Target Genes J. Biol. Chem., August 25, 2006; 281(34): 24138 - 24148. [Abstract] [Full Text] [PDF]