This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bera, T. K. Articles by Pastan, I. Search for Related Content PubMed PubMed Citation Articles by Bera, T. K. Articles by Pastan, I.

POTE Paralogs Are Induced and Differentially Expressed in Many Cancers

¹ Laboratories of Molecular Biology and ² Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland

Requests for reprints: Ira Pastan, Laboratory of Molecular Biology, National Cancer Institute, Room 5106, 37 Convent Drive, Bethesda, MD 20892-4264. Phone: 301-496-4797; Fax: 301-402-1344; E-mail: pastani{at}mail.nih.gov.

To identify new antigens that are targets for the immunotherapy of prostate and breast cancer, we used expressed sequence tag and genomic databases and discovered POTE, a new primate-specific gene family. Each POTE gene encodes a protein that contains three domains, although the proteins vary greatly in size. The NH₂-terminal domain is novel and has properties of an extracellular domain but does not contain a signal sequence. The second and third domains are rich in ankyrin repeats and spectrin-like helices, respectively. The protein encoded by POTE-21, the first family member discovered, is localized on the plasma membrane of the cell. In humans, 13 highly homologous paralogs are dispersed among eight chromosomes. The expression of POTE genes in normal tissues is restricted to prostate, ovary, testis, and placenta. A survey of several cancer samples showed that POTE was expressed in 6 of 6 prostate, 12 of 13 breast, 5 of 5 colon, 5 of 6 lung, and 4 of 5 ovarian cancers. To determine the relative expression of each POTE paralog in cancer and normal samples, we employed a PCR-based cloning and analysis method. We found that POTE-2, POTE-2ß, POTE-2, and POTE-22 are predominantly expressed in cancers whereas POTE expression in normal tissues is somewhat more diverse. Because POTE is primate specific and is expressed in testis and many cancers but only in a few normal tissues, we conclude POTE is a new primate-specific member of the cancer-testis antigen family. It is likely that POTE has a unique role in primate biology. (Cancer Res 2006; 66(1): 52-6)

This article has been cited by other articles:

				T. K. Bera, X.-F. Liu, M. Yamada, O. Gavrilova, E. Mezey, L. Tessarollo, M. Anver, Y. Hahn, B. Lee, and I. Pastan A model for obesity and gigantism due to disruption of the Ankrd26 gene PNAS, January 8, 2008; 105(1): 270 - 275. [Abstract] [Full Text] [PDF]

				Y. Lee, T. Ise, D. Ha, A. Saint Fleur, Y. Hahn, X.-F. Liu, S. Nagata, B. Lee, T. K. Bera, and I. Pastan Evolution and expression of chimeric POTE-actin genes in the human genome PNAS, November 21, 2006; 103(47): 17885 - 17890. [Abstract] [Full Text] [PDF]