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Epidemiology and Prevention |
1 Institut National de la Santé et de la Recherche Médicale, INSERM U670, IFR 50, Faculté de Médecine; 2 Centre National de la Recherche Scientifique Unité Mixte de Recherche 6548, Faculté des Sciences; 3 Institut National de la Santé et de la Recherche Médicale, U627, IFR 50; 4 Institut National de la Santé et de la Recherche Médicale/Région Provence Alpes Côte d' Azur, ESPRI 2006/Laboratoire de Pathologie Clinique et Expérimentale, Hôpital Pasteur, Nice, France; and 5 Groupe Appareil Digestif et Environnement (EA3234), Faculté de Médecine, Rouen, France
Requests for reprints: Baharia Mograbi, Institut National de la Santé et de la Recherche Médicale ESPRI 2006, IFR 50, Faculté de Médecine, Avenue de Valombrose, 06107 Nice Cedex 02, France. Phone: 33-4-93-37-77-94; Fax: 33-4-93-37-77-52; E-mail: mograbi{at}hermes.unice.fr.
Macroautophagy (hereafter referred to as autophagy) has emerged as a key tumor suppressor pathway. During this process, the cytosolic constituents are sequestered into autophagosomes, which subsequently fuse with lysosomes to become autolysosomes where their contents are finally degraded. Although a reduced autophagy has been shown in human tumors or in response to oncogenes and carcinogens, the underlying mechanism(s) remain(s) unknown. Here, we show that widely used carcinogen Lindane promotes vacuolation of Sertoli cells. By electron and immunofluorescent microscopy analyses, we showed that these structures are acid autolysosomes, containing cellular debris, and labeled by LC3, Rab7, and LAMP1, markers of autophagosomes, late endosomes, and lysosomes, respectively. Such Lindane-induced vacuolation results from significant delay in autophagy degradation, in relation with a decline of the lysosomal activity of aryl sulfatase A. At molecular level, we show that this defect in autolysosomal maturation is independent of mammalian target of rapamycin and p38 inhibitions. Rather, the activation of the mitogen-activated protein kinase (MAPK)/extracellular signalregulated kinase (ERK) pathway is required for Lindane to disrupt the autophagic pathway. Most importantly, we provide the first evidence that sustained activation of ERK pathway is sufficient to commit cell to autophagic vacuolation. Taken together, these findings strongly support that the aberrant sustained activation of ERK by the carcinogen Lindane disrupts the maturation of autophagosomes into functional autolysosomes. Our findings therefore suggest the possibility that high constitutive ERK activity found in all cancers may provide a malignant advantage by impeding the tumor suppressive function of autophagy. (Cancer Res 2006; 66(13): 6861-70)
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