This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wu, J. Articles by Huang, T. H-M. Search for Related Content PubMed PubMed Citation Articles by Wu, J. Articles by Huang, T. H-M. Related Collections Cellular Pathobiology Cellular Pathobiology: Cancer Genes and Genomics

ChIP-chip Comes of Age for Genome-wide Functional Analysis

Departments of ¹ Molecular Genetics and ² Molecular Virology, Immunology, and Medical Genetics, Division of Human Cancer Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, Ohio

Requests for reprints: Tim H-M. Huang or Christoph Plass, Human Cancer Genetics Program, The Ohio State University, 420 West 12th Avenue, Columbus, OH 43210. E-mail: tim.huang{at}osumc.edu or christoph.plass@osumc.edu.

In the post-genome era, attention has focused on the functions of genome sequences and how they are regulated. The emerging epigenomic changes and the interactions between cis-acting elements and protein factors may play a central role in gene regulation. To understand the crosstalk between DNA and protein on a genome-wide scale, one emerging technique, called ChIP-chip, takes the strategy of combining chromatin immunoprecipitation with microarray. This new high-throughput strategy helps screen the targets of critical transcription factors and profile the genome-wide distribution of histone modifications, which will enable the feasibility of conducting a large-scale study, such as the Human Epigenome Project. (Cancer Res 2006; 66(14): 6899-902)

This article has been cited by other articles:

				C. Linhart, Y. Halperin, and R. Shamir Transcription factor and microRNA motif discovery: The Amadeus platform and a compendium of metazoan target sets Genome Res., July 1, 2008; 18(7): 1180 - 1189. [Abstract] [Full Text] [PDF]

				D. B. Veprintsev and A. R. Fersht Algorithm for prediction of tumour suppressor p53 affinity for binding sites in DNA Nucleic Acids Res., March 1, 2008; 36(5): 1589 - 1598. [Abstract] [Full Text] [PDF]

				M. Sakaguchi, H. Sonegawa, H. Murata, M. Kitazoe, J.-i. Futami, K. Kataoka, H. Yamada, and N.-h. Huh S100A11, an Dual Mediator for Growth Regulation of Human Keratinocytes Mol. Biol. Cell, January 1, 2008; 19(1): 78 - 85. [Abstract] [Full Text] [PDF]

				D. M. Lonard, R. B. Lanz, and B. W. O'Malley Nuclear Receptor Coregulators and Human Disease Endocr. Rev., August 1, 2007; 28(5): 575 - 587. [Abstract] [Full Text] [PDF]

				M. Kininis, B. S. Chen, A. G. Diehl, G. D. Isaacs, T. Zhang, A. C. Siepel, A. G. Clark, and W. L. Kraus Genomic Analyses of Transcription Factor Binding, Histone Acetylation, and Gene Expression Reveal Mechanistically Distinct Classes of Estrogen-Regulated Promoters Mol. Cell. Biol., July 15, 2007; 27(14): 5090 - 5104. [Abstract] [Full Text] [PDF]

				G. M. Euskirchen, J. S. Rozowsky, C.-L. Wei, W. H. Lee, Z. D. Zhang, S. Hartman, O. Emanuelsson, V. Stolc, S. Weissman, M. B. Gerstein, et al. Mapping of transcription factor binding regions in mammalian cells by ChIP: Comparison of array- and sequencing-based technologies Genome Res., June 1, 2007; 17(6): 898 - 909. [Abstract] [Full Text] [PDF]

				J. Qiu, S. Yao, C. Hindmarch, V. Antunes, J. Paton, and D. Murphy Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A J. Neurosci., February 28, 2007; 27(9): 2196 - 2203. [Abstract] [Full Text] [PDF]