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Daxx Represses Expression of a Subset of Antiapoptotic Genes Regulated by Nuclear Factor-B

Burnham Institute for Medical Research, La Jolla, California

Requests for reprints: John C. Reed, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037. Phone: 858-646-5300; Fax: 858-646-3194; E-mail: reedoffice{at}burnham.org.

Daxx is a nuclear protein that localizes to PML oncogenic domains, sensitizes cells to apoptosis, and functions as a transcriptional repressor. We found that Daxx represses the expression of several antiapoptotic genes regulated by nuclear factor-B, including cIAP2, in human tumor cell lines. Daxx interacts with RelB and inhibits RelB-mediated transcriptional activation of the human cIAP2 gene promoter. Daxx also forms complexes with RelB while bound to its target sites in the cIAP2 promoter, as shown by electrophoretic mobility shift assays and chromatin immunoprecipitation experiments. Using cells from daxx–/– mouse embryos, we observed that levels of the corresponding murine c-IAP mRNA and protein are increased in cells lacking Daxx. Conversely, c-IAP mRNA and protein levels were reduced in relB–/– cells. Taken together, these observations provide a mechanism that links two previously ascribed functions of Daxx: transcriptional repression and sensitization to apoptosis. (Cancer Res 2006; 66(18): 9026-35)

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				L. A. Puto and J. C. Reed Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation Genes & Dev., April 15, 2008; 22(8): 998 - 1010. [Abstract] [Full Text] [PDF]