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Influence of Human CD8 on Antigen Recognition by T-Cell Receptor–Transduced Cells

Department of Surgery, The University of Chicago, Chicago, Illinois

Requests for reprints: Michael I. Nishimura, Department of Surgery, Medical University of South Carolina, Hollings Cancer Center, 86 Jonathon Lucas Street, Suite 512, P.O. Box 250613, Charleston, SC 29425. Phone: 843-792-7789; Fax: 843-792-2556; E-mail: nishimum{at}musc.edu.

The CD8 coreceptor on T cells has two functions. Namely, CD8 acts to stabilize the binding of the T-cell receptor (TCR) to the peptide-MHC complex while localizing p56^lck (lck) to the TCR/CD3 complex to facilitate early signaling events. Although both functions may be critical for efficient activation of a CTL, little is known about how the structural versus signaling roles of CD8, together with the relative strength of the TCR, influences T-cell function. We have addressed these issues by introducing full-length and truncated versions of the CD8 and CD8ß chains into CD8^– Jurkat cell clones expressing cloned TCRs with known antigen specificity and relative affinities. Using a combination of antigen recognition and tetramer-binding assays, we find that the intracellular lck-binding domain of CD8 is critical for enhanced T-cell activation regardless of the relative strength of the TCR. In contrast, the extracellular domain of CD8 seems to be critical for TCRs with lower affinity but not those with higher affinity. Based on our results, we conclude that there are different requirements for CD8 to enhance T-cell function depending on the strength of its TCR. (Cancer Res 2006; 66(23): 11455-61)

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