| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Epidemiology and Prevention |
1 Diet and Cancer Group, Dunn Human Nutrition Unit, Wellcome Trust/Medical Research Council Building, Cambridge, United Kingdom; 2 Pollock and Pool Ltd., Reading, Berkshire, United Kingdom; and 3 Department of Chemistry, The Open University, Walton Hall, Milton Keynes, United Kingdom
Requests for reprints: Sheila A. Bingham, Dunn Human Nutrition Unit, Wellcome Trust/Medical Research Council Building, Cambridge, United Kingdom CB2 2XY. Phone: 44-1223-252760; Fax: 44-1223-252765; E-mail: sab{at}mrc-dunn.cam.ac.uk.
Red meat is associated with increased risk of colorectal cancer and increases the endogenous formation of N-nitrosocompounds (NOC). To investigate the genotoxic effects of NOC arising from red meat consumption, human volunteers were fed high (420 g) red meat, vegetarian, and high red meat, high-fiber diets for 15 days in a randomized crossover design while living in a volunteer suite, where food was carefully controlled and all specimens were collected. In 21 volunteers, there was a consistent and significant (P < 0.0001) increase in endogenous formation of NOC with the red meat diet compared with the vegetarian diet as measured by apparent total NOC (ATNC) in feces. In colonic exfoliated cells, the percentage staining positive for the NOC-specific DNA adduct, O6-carboxymethyl guanine (O6CMG) was significantly (P < 0.001) higher on the high red meat diet. In 13 volunteers, levels were intermediate on the high-fiber, high red meat diet. Fecal ATNC were positively correlated with the percentage of cells staining positive for O6CMG (r2 = 0.56, P = 0.011). The presence of O6CMG was also shown in intact small intestine from rats treated with the N-nitrosopeptide N-acetyl-N'-prolyl-N'-nitrosoglycine and in HT-29 cells treated with diazoacetate. This study has shown that fecal NOC arising from red meat include direct acting diazopeptides or N-nitrosopeptides able to form alkylating DNA adducts in the colon. As these O6CMG adducts are not repaired, and if other related adducts are formed and not repaired, this may explain the association of red meat with colorectal cancer. (Cancer Res 2006; 66(3): 1859-65)
This article has been cited by other articles:
|
|
 |
|
  R. Sinha, Y. Park, B. I. Graubard, M. F. Leitzmann, A. Hollenbeck, A. Schatzkin, and A. J. Cross Meat and Meat-related Compounds and Risk of Prostate Cancer in a Large Prospective Cohort Study in the United States Am. J. Epidemiol., November 1, 2009; 170(9): 1165 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M.C.P. Joosen, G. G.C. Kuhnle, S. M. Aspinall, T. M. Barrow, E. Lecommandeur, A. Azqueta, A. R. Collins, and S. A. Bingham Effect of processed and red meat on endogenous nitrosation and DNA damage Carcinogenesis, August 1, 2009; 30(8): 1402 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Billson, K. L. Harrison, N. P. Lees, C. N. Hall, G. P. Margison, and A. C. Povey Dietary variables associated with DNA N7-methylguanine levels and O6-alkylguanine DNA-alkyltransferase activity in human colorectal mucosa Carcinogenesis, April 1, 2009; 30(4): 615 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Joshi, R. Corral, K. D. Siegmund, R. W. Haile, L. Le Marchand, M. E. Martinez, D. J. Ahnen, R. S. Sandler, P. Lance, and M. C. Stern Red meat and poultry intake, polymorphisms in the nucleotide excision repair and mismatch repair pathways and colorectal cancer risk Carcinogenesis, March 1, 2009; 30(3): 472 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Wild Environmental exposure measurement in cancer epidemiology Mutagenesis, March 1, 2009; 24(2): 117 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang and Y. Wang Chemical synthesis of oligodeoxyribonucleotides containing N3- and O4-carboxymethylthymidine and their formation in DNA Nucleic Acids Res., February 1, 2009; 37(2): 336 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Padler-Karavani, H. Yu, H. Cao, H. Chokhawala, F. Karp, N. Varki, X. Chen, and A. Varki Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease Glycobiology, October 1, 2008; 18(10): 818 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N P Lees, K L Harrison, C N Hall, G P Margison, and A C Povey Human colorectal mucosal O6-alkylguanine DNA-alkyltransferase activity and DNA-N7-methylguanine levels in colorectal adenoma cases and matched referents Gut, March 1, 2007; 56(3): 380 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Goode, J. D. Potter, W. R. Bamlet, D. N. Rider, and J. Bigler Inherited variation in carcinogen-metabolizing enzymes and risk of colorectal polyps Carcinogenesis, February 1, 2007; 28(2): 328 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gottschalg, G. B. Scott, P. A. Burns, and D. E.G. Shuker Potassium diazoacetate-induced p53 mutations in vitro in relation to formation of O6-carboxymethyl- and O6-methyl-2'-deoxyguanosine DNA adducts: relevance for gastrointestinal cancer Carcinogenesis, February 1, 2007; 28(2): 356 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Pierre, G. Peiro, S. Tache, A. J. Cross, S. A. Bingham, N. Gasc, G. Gottardi, D. E. Corpet, and F. Gueraud New Marker of Colon Cancer Risk Associated with Heme Intake: 1,4-Dihydroxynonane Mercapturic Acid. Cancer Epidemiol. Biomarkers Prev., November 1, 2006; 15(11): 2274 - 2279. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
