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Prognostic Value of an Activation State Marker for Epidermal Growth Factor Receptor in Tissue Microarrays of Head and Neck Cancer

¹ Cell Biophysics Lab, ² Protein Phosphorylation Lab, London Research Institute, Cancer Research UK, London, United Kingdom; ³ Weatherall Institute of Molecular Medicine, Cancer Research UK, University of Oxford, John Radcliffe Hospital, Headley Way, Oxford, United Kingdom; and ⁴ Laboratoire de Physiologie de la Pérception et de l'Action, College de France, Paris, France

Requests for reprints: Banafshé Larijani, Cell Biophysics, 44 Lincoln's Inn Fields, Cancer Research UK, London, WC2A 3PX, United Kingdom. Phone: 44-20-7269-3082; Fax: 44-20-7269-3094; E-mail: banafshe.larijani{at}cancer.org.uk.

Overexpression and mutation of epidermal growth factor receptors (EGFR) have been shown to be important in the prognosis of several cancers, including head and neck cancers. However, our inability to define the activation status of these and other receptors limits our ability to assess the importance of these pathways and to exploit effectively new molecularly targeted treatments directed at their catalytic activities. Here we describe the use of automated, high-throughput fluorescence lifetime imaging microscopy to measure EGFR autophosphorylation status by fluorescence resonance energy transfer (FRET) in head and neck tumors. We have correlated FRET efficiency with the clinical and survival data. The results from head and neck arrays show that high FRET efficiency is correlated with worsening disease-free survival but not with overall survival. This powerful tool could be exploited as a new independent quantitative prognostic factor in clinical decisions and cancer management. (Cancer Res 2006; 66(5): 2834-43)

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