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Background Mutation Frequency in Microsatellite-Unstable Colorectal Cancer

¹ Department of Medical Genetics, Biomedicum Helsinki, University of Helsinki; ² Second Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland; ³ CSC, Finnish IT Center for Science, Espoo, Finland; ⁴ Department of Surgery, Jyväskylä Central Hospital, Jyväskylä, Finland; and ⁵ Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland

Requests for reprints: Lauri A. Aaltonen, Department of Medical Genetics, Biomedicum Helsinki, University of Helsinki, P.O. Box 63, FIN-00014 Helsinki, Finland. Phone: 358-91912-5595; Fax: 358-91912-5105; E-mail: lauri.aaltonen{at}helsinki.fi.

Microsatellite instability (MSI) is observed in 12% of colorectal cancers. Genes containing a mononucleotide microsatellite in the coding sequence are particularly prone to inactivation in MSI tumorigenesis, and much work has been conducted to identify genes with high repetitive tract mutation rates in these tumors. Much less attention has been paid to background mutation frequencies, and no work has focused on nontranscribed regions. Here, we studied 114 nontranscribed intergenic A/T and C/G repeats 6 to 10 bp in length, located distant from known genes, to examine background mutation frequencies in MSI colorectal cancers. A strong correlation with tract length was observed, and mutation frequencies of up to 87% were observed in 8 to 10 bp tracts. Subsequently, to compare the background mutation rate in transcribed and nontranscribed noncoding repeats, we screened nine randomly selected intronic C/G8 repeats. In addition, the coding repeats of seven suggested MSI target genes, and nine previously published intronic A8 and G8 repeats were analyzed. Intronic repeats seemed to mutate less frequently than nontranscribed intergenic repeats. Our results show that strand slippage mutations in mismatch repair–deficient cells are as abundant in short intergenic repeats as in many proposed MSI target genes. However, under mismatch repair deficiency, strand slippage mutations in transcribed sequences seem to be repaired more efficiently than in intergenic nontranscribed sequences. The mechanisms causing these differences are not yet understood and should be a subject for further studies. For MSI target gene identification, repeats in transcribed sequences seem to be the most appropriate reference group for coding region repeat mutations. [Cancer Res 2007;67(12):5691–8]

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