This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pfenninger, C. V. Articles by Nuber, U. A. Search for Related Content PubMed PubMed Citation Articles by Pfenninger, C. V. Articles by Nuber, U. A.

CD133 Is Not Present on Neurogenic Astrocytes in the Adult Subventricular Zone, but on Embryonic Neural Stem Cells, Ependymal Cells, and Glioblastoma Cells

¹ Lund Strategic Research Center for Stem Cell Biology, Lund University; ² Division of Neuropathology, Department of Pathology and Cytology, Lund University Hospital; and Departments of ³ Neurosurgery and ⁴ Oncology, University Hospital, Lund, Sweden

Requests for reprints: Ulrike A. Nuber, Lund Strategic Research Center for Stem Cell Biology, Klinikgatan 26, 221 84 Lund, Sweden. Phone: 46-46-2221774; E-mail: ulrike.nuber{at}med.lu.se.

Human brain tumor stem cells have been enriched using antibodies against the surface protein CD133. An antibody recognizing CD133 also served to isolate normal neural stem cells from fetal human brain, suggesting a possible lineage relationship between normal neural and brain tumor stem cells. Whether CD133-positive brain tumor stem cells can be derived from CD133-positive neural stem or progenitor cells still requires direct experimental evidence, and an important step toward such investigations is the identification and characterization of normal CD133-presenting cells in neurogenic regions of the embryonic and adult brain. Here, we present evidence that CD133 is a marker for embryonic neural stem cells, an intermediate radial glial/ependymal cell type in the early postnatal stage, and for ependymal cells in the adult brain, but not for neurogenic astrocytes in the adult subventricular zone. Our findings suggest two principal possibilities for the origin of brain tumor stem cells: a derivation from CD133-expressing cells, which are normally not present in the adult brain (embryonic neural stem cells and an early postnatal intermediate radial glial/ependymal cell type), or from CD133-positive ependymal cells in the adult brain, which are, however, generally regarded as postmitotic. Alternatively, brain tumor stem cells could be derived from proliferative but CD133-negative neurogenic astrocytes in the adult brain. In the latter case, brain tumor development would involve the production of CD133. [Cancer Res 2007;67(12):5727–36]

This article has been cited by other articles:

				T. A. Prokhorova, K. T. G. Rigbolt, P. T. Johansen, J. Henningsen, I. Kratchmarova, M. Kassem, and B. Blagoev Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Quantitative Comparison of the Membrane Proteomes of Self-renewing and Differentiating Human Embryonic Stem Cells Mol. Cell. Proteomics, May 1, 2009; 8(5): 959 - 970. [Abstract] [Full Text] [PDF]

				B. A. Emmenegger and R. J. Wechsler-Reya Stem Cells and the Origin and Propagation of Brain Tumors J Child Neurol, October 1, 2008; 23(10): 1172 - 1178. [Abstract] [PDF]

				B. M. Howard, Zhicheng Mo, R. Filipovic, A. R. Moore, S. D. Antic, and N. Zecevic Radial Glia Cells in the Developing Human Brain Neuroscientist, October 1, 2008; 14(5): 459 - 473. [Abstract] [PDF]