This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Porkka, K. P. Articles by Visakorpi, T. Search for Related Content PubMed PubMed Citation Articles by Porkka, K. P. Articles by Visakorpi, T.

MicroRNA Expression Profiling in Prostate Cancer

¹ Laboratory of Cancer Genetics, Institute of Medical Technology and ² Department of Urology, University of Tampere and Tampere University Hospital, Tampere, Finland and ³ Department of Urology, University of Washington; ⁴ Puget Sound Veterans Affairs Medical System, Seattle, Washington

Requests for reprints: Tapio Visakorpi, Institute of Medical Technology, FIN-33014 University of Tampere, Tampere, Finland. Phone: 358-3-3551-7725; Fax: 358-3-3551-8597; E-mail: tapio.visakorpi{at}uta.fi.

MicroRNAs (miRNA) are small, endogenously expressed noncoding RNAs that negatively regulate expression of protein-coding genes at the translational level. Accumulating evidence, such as aberrant expression of miRNAs, suggests that they are involved in the development of cancer. They have been identified in various tumor types, showing that different sets of miRNAs are usually deregulated in different cancers. To identify the miRNA signature specific for prostate cancer, miRNA expression profiling of 6 prostate cancer cell lines, 9 prostate cancer xenografts samples, 4 benign prostatic hyperplasia (BPH), and 9 prostate carcinoma samples was carried out by using an oligonucleotide array hybridization method. Differential expression of 51 individual miRNAs between benign tumors and carcinoma tumors was detected, 37 of them showing down-regulation and 14 up-regulation in carcinoma samples, thus identifying those miRNAs that could be significant in prostate cancer development and/or growth. There was a significant trend (P = 0.029) between the expression of miRNAs and miRNA locus copy number determined by array comparative genomic hybridization, indicating that genetic aberrations may target miRNAs. Hierarchical clustering of the tumor samples by their miRNA expression accurately separated the carcinomas from the BPH samples and also further classified the carcinoma tumors according to their androgen dependence (hormone naive versus hormone refractory), indicating the potential of miRNAs as a novel diagnostic and prognostic tool for prostate cancer. [Cancer Res 2007;67(13):6130–5]

This article has been cited by other articles:

				T. Sun, Q. Wang, S. Balk, M. Brown, G.-S. M. Lee, and P. Kantoff The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines Cancer Res., April 15, 2009; 69(8): 3356 - 3363. [Abstract] [Full Text] [PDF]

				C. L. Bartels and G. J. Tsongalis MicroRNAs: Novel Biomarkers for Human Cancer Clin. Chem., April 1, 2009; 55(4): 623 - 631. [Abstract] [Full Text] [PDF]

				P. Gandellini, M. Folini, N. Longoni, M. Pennati, M. Binda, M. Colecchia, R. Salvioni, R. Supino, R. Moretti, P. Limonta, et al. miR-205 Exerts Tumor-Suppressive Functions in Human Prostate through Down-regulation of Protein Kinase C{varepsilon} Cancer Res., March 15, 2009; 69(6): 2287 - 2295. [Abstract] [Full Text] [PDF]

				J. M. Friedman, G. Liang, C.-C. Liu, E. M. Wolff, Y. C. Tsai, W. Ye, X. Zhou, and P. A. Jones The Putative Tumor Suppressor microRNA-101 Modulates the Cancer Epigenome by Repressing the Polycomb Group Protein EZH2 Cancer Res., March 15, 2009; 69(6): 2623 - 2629. [Abstract] [Full Text] [PDF]

				F. Kuchenbauer, R. D. Morin, B. Argiropoulos, O. I. Petriv, M. Griffith, M. Heuser, E. Yung, J. Piper, A. Delaney, A.-L. Prabhu, et al. In-depth characterization of the microRNA transcriptome in a leukemia progression model Genome Res., November 1, 2008; 18(11): 1787 - 1797. [Abstract] [Full Text] [PDF]

				S. Kim, N. Kim, B. Dong, D. Boren, S. A. Lee, J. Das Gupta, C. Gaughan, E. A. Klein, C. Lee, R. H. Silverman, et al. Integration Site Preference of Xenotropic Murine Leukemia Virus-Related Virus, a New Human Retrovirus Associated with Prostate Cancer J. Virol., October 15, 2008; 82(20): 9964 - 9977. [Abstract] [Full Text] [PDF]

				S. Ambs, R. L. Prueitt, M. Yi, R. S. Hudson, T. M. Howe, F. Petrocca, T. A. Wallace, C.-G. Liu, S. Volinia, G. A. Calin, et al. Genomic Profiling of MicroRNA and Messenger RNA Reveals Deregulated MicroRNA Expression in Prostate Cancer Cancer Res., August 1, 2008; 68(15): 6162 - 6170. [Abstract] [Full Text] [PDF]

				P. S. Mitchell, R. K. Parkin, E. M. Kroh, B. R. Fritz, S. K. Wyman, E. L. Pogosova-Agadjanyan, A. Peterson, J. Noteboom, K. C. O'Briant, A. Allen, et al. Circulating microRNAs as stable blood-based markers for cancer detection PNAS, July 29, 2008; 105(30): 10513 - 10518. [Abstract] [Full Text] [PDF]

				M. N. Nikiforova, G. C. Tseng, D. Steward, D. Diorio, and Y. E. Nikiforov MicroRNA Expression Profiling of Thyroid Tumors: Biological Significance and Diagnostic Utility J. Clin. Endocrinol. Metab., May 1, 2008; 93(5): 1600 - 1608. [Abstract] [Full Text] [PDF]

				R. D. Morin, M. D. O'Connor, M. Griffith, F. Kuchenbauer, A. Delaney, A.-L. Prabhu, Y. Zhao, H. McDonald, T. Zeng, M. Hirst, et al. Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells Genome Res., April 1, 2008; 18(4): 610 - 621. [Abstract] [Full Text] [PDF]

				S.-L. Lin, A. Chiang, D. Chang, and S.-Y. Ying Loss of mir-146a function in hormone-refractory prostate cancer RNA, March 1, 2008; 14(3): 417 - 424. [Abstract] [Full Text] [PDF]