This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yoo, C. B. Articles by Jones, P. A. Search for Related Content PubMed PubMed Citation Articles by Yoo, C. B. Articles by Jones, P. A.

Delivery of 5-Aza-2'-Deoxycytidine to Cells Using Oligodeoxynucleotides

Departments of ¹ Biochemistry and Molecular Biology and ² Urology and ³ Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California and ⁴ SuperGen, Inc., Pleasanton, California

Requests for reprints: Peter A. Jones, University of Southern California/Norris Comprehensive Cancer Center and Hospital, 1441 Eastlake Avenue, Room 8302L, Mail Stop 83, Los Angeles, CA 90033-9181. Phone: 323-865-0816; Fax: 323-865-0102; E-mail: jones_p{at}ccnt.hsc.usc.edu.

The major goal of epigenetic therapy is to reverse aberrant promoter hypermethylation and restore normal function of tumor suppressor genes by the use of chromatin-modifying drugs. Decitabine, or 5-aza-2'-deoxycytidine (5-aza-CdR), is a well-characterized drug that is now Food and Drug Administration approved for the treatment of myelodysplastic syndrome. Although 5-aza-CdR is an extremely potent inhibitor of DNA methylation, it is subject to degradation by hydrolytic cleavage and deamination by cytidine deaminase. We show that short oligonucleotides containing a 5-aza-CdR can also inhibit DNA methylation in cancer cells at concentrations comparable with 5-aza-CdR. Detailed studies with S110, a dinucleotide, showed that it works via a mechanism similar to that of 5-aza-CdR after incorporation of its aza-moiety into DNA. Stability of the triazine ring in aqueous solution was not improved in the S110 dinucleotide; however, deamination by cytidine deaminase was dramatically decreased. This is the first demonstration of the use of short oligonucleotides to provide effective delivery and cellular uptake of a nucleotide drug and protection from enzymatic degradation. This approach may pave the way for more stable and potent inhibitors of DNA methylation as well as provide means for improving existing therapeutics. [Cancer Res 2007;67(13):6400–8]