Cancer Research Translational Cancer Medicine 2008: Cancer Clinical Trials and Personalized Medicine  AACR Conference on Molecular Diagnostics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation

Cancer Research 67, 7713-7722, August 15, 2007. doi: 10.1158/0008-5472.CAN-07-1083
© 2007 American Association for Cancer Research

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, C. D.
Right arrow Articles by Slack, F. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, C. D.
Right arrow Articles by Slack, F. J.

Cell, Tumor, and Stem Cell Biology

The let-7 MicroRNA Represses Cell Proliferation Pathways in Human Cells

Charles D. Johnson1, Aurora Esquela-Kerscher3, Giovanni Stefani3, Mike Byrom1, Kevin Kelnar1, Dmitriy Ovcharenko1, Mike Wilson1, Xiaowei Wang2, Jeffrey Shelton1, Jaclyn Shingara1, Lena Chin3, David Brown1 and Frank J. Slack3

1 Asuragen, Inc.; 2 Ambion, Inc., Austin, Texas, and 3 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut

Requests for reprints: Frank J. Slack, Department of Molecular, Cellular and Developmental Biology, P.O. Box 208103, Yale University, New Haven, CT 06520. Phone: 203-432-3492; Fax: 203-432-6161; E-mail: frank.slack{at}yale.edu or David Brown, Asuragen, Inc., 2150 Woodward St., Austin, TX 78744. E-mail: dbrown{at}asuragen.com.

MicroRNAs play important roles in animal development, cell differentiation, and metabolism and have been implicated in human cancer. The let-7 microRNA controls the timing of cell cycle exit and terminal differentiation in Caenorhabditis elegans and is poorly expressed or deleted in human lung tumors. Here, we show that let-7 is highly expressed in normal lung tissue, and that inhibiting let-7 function leads to increased cell division in A549 lung cancer cells. Overexpression of let-7 in cancer cell lines alters cell cycle progression and reduces cell division, providing evidence that let-7 functions as a tumor suppressor in lung cells. let-7 was previously shown to regulate the expression of the RAS lung cancer oncogenes, and our work now shows that multiple genes involved in cell cycle and cell division functions are also directly or indirectly repressed by let-7. This work reveals the let-7 microRNA to be a master regulator of cell proliferation pathways. [Cancer Res 2007;67(16):7713–22]




This article has been cited by other articles:


Home page
Cancer Res.Home page
B. Boyerinas, S.-M. Park, N. Shomron, M. M. Hedegaard, J. Vinther, J. S. Andersen, C. Feig, J. Xu, C. B. Burge, and M. E. Peter
Identification of Let-7-Regulated Oncofetal Genes
Cancer Res., April 15, 2008; 68(8): 2587 - 2591.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Cevec, C. Thibaudeau, and J. Plavec
Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans
Nucleic Acids Res., April 1, 2008; 36(7): 2330 - 2337.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
S.-M. Park, A. B. Gaur, E. Lengyel, and M. E. Peter
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2
Genes & Dev., April 1, 2008; 22(7): 894 - 907.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. S. Kumar, S. J. Erkeland, R. E. Pester, C. Y. Chen, M. S. Ebert, P. A. Sharp, and T. Jacks
Suppression of non-small cell lung tumor development by the let-7 microRNA family
PNAS, March 11, 2008; 105(10): 3903 - 3908.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. B. Weidhaas, I. Babar, S. M. Nallur, P. Trang, S. Roush, M. Boehm, E. Gillespie, and F. J. Slack
MicroRNAs as Potential Agents to Alter Resistance to Cytotoxic Anticancer Therapy
Cancer Res., December 1, 2007; 67(23): 11111 - 11116.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation
Copyright © 2007 by the American Association for Cancer Research.