This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jones, R. Articles by Peters, G. Search for Related Content PubMed PubMed Citation Articles by Jones, R. Articles by Peters, G.

A CDKN2A Mutation in Familial Melanoma that Abrogates Binding of p16^INK4a to CDK4 but not CDK6

¹ Molecular Oncology Laboratory, Cancer Research UK London Research Institute and ² Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy

Requests for reprints: Gordon Peters, CRUK London Research Institute, Lincolns Inn Field London, WC2A 3PX, United Kingdom. Phone: 44-0207-269-3049; Fax: 44-0207-269-3094; E-mail: gordon.peters{at}cancer.org.uk.

The CDKN2A locus encodes two distinct proteins, p16^INK4a and p14^ARF, both of which are implicated in replicative senescence and tumor suppression in different contexts. Here, we describe the characterization of a novel strain of human diploid fibroblasts (designated Milan HDFs) from an individual who is homozygous for the R24P mutation in p16^INK4a. As this mutation occurs in the first exon of INK4a (exon 1), it has no effect on the primary sequence of p14^ARF. Based on both in vitro and in vivo analyses, the R24P variant is specifically defective for binding to CDK4 but remains able to associate with CDK6. Nevertheless, Milan HDFs behave as if they are p16^INK4a deficient, in terms of sensitivity to spontaneous and oncogene-induced senescence, and the R24P variant has little effect on proliferation when ectopically expressed in normal fibroblasts. It can, however, impair the proliferation of U20S cells, presumably because they express more CDK6 than primary fibroblasts. These observations suggest that CDK4 and CDK6 are not functionally redundant and underscore the importance of CDK4 in the development of melanoma. [Cancer Res 2007;67(19):9134–41]

This article has been cited by other articles:

				A. Baldwin, W. Li, M. Grace, J. Pearlberg, E. Harlow, K. Munger, and D. A. Grueneberg Kinase requirements in human cells: II. Genetic interaction screens identify kinase requirements following HPV16 E7 expression in cancer cells PNAS, October 28, 2008; 105(43): 16478 - 16483. [Abstract] [Full Text] [PDF]