This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Medina, R. Articles by Stein, G. S. Search for Related Content PubMed PubMed Citation Articles by Medina, R. Articles by Stein, G. S.

The HiNF-P/p220^NPAT Cell Cycle Signaling Pathway Controls Nonhistone Target Genes

Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts

Requests for reprints: Gary S. Stein, Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655. Phone: 508-856-5625; Fax: 508-856-6800; E-mail: Gary.Stein{at}umassmed.edu.

HiNF-P and its cofactor p220^NPAT are principal factors regulating histone gene expression at the G₁-S phase cell cycle transition. Here, we have investigated whether HiNF-P controls other cell cycle– and cancer-related genes. We used cDNA microarrays to monitor responsiveness of gene expression to small interfering RNA–mediated depletion of HiNF-P. Candidate HiNF-P target genes were examined for the presence of HiNF-P recognition motifs, in vitro HiNF-P binding to DNA, and in vivo association by chromatin immunoprecipitations and functional reporter gene assays. Of 177 proliferation-related genes we tested, 20 are modulated in HiNF-P–depleted cells and contain putative HiNF-P binding motifs. We validated that at least three genes (i.e., ATM, PRKDC, and CKS2) are HiNF-P dependent and provide data indicating that the DNA damage response is altered in HiNF-P–depleted cells. We conclude that, in addition to histone genes, HiNF-P also regulates expression of nonhistone targets that influence competency for cell cycle progression. [Cancer Res 2007;67(21):10334–42]

This article has been cited by other articles:

				R. Xie, R. Medina, Y. Zhang, S. Hussain, J. Colby, P. Ghule, S. Sundararajan, M. Keeler, L.-J. Liu, M. van der Deen, et al. The histone gene activator HINFP is a nonredundant cyclin E/CDK2 effector during early embryonic cell cycles PNAS, July 28, 2009; 106(30): 12359 - 12364. [Abstract] [Full Text] [PDF]

				P. N. Ghule, Z. Dominski, X.-c. Yang, W. F. Marzluff, K. A. Becker, J. W. Harper, J. B. Lian, J. L. Stein, A. J. van Wijnen, and G. S. Stein Staged assembly of histone gene expression machinery at subnuclear foci in the abbreviated cell cycle of human embryonic stem cells PNAS, November 4, 2008; 105(44): 16964 - 16969. [Abstract] [Full Text] [PDF]