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Novel Indenoisoquinolines NSC 725776 and NSC 724998 Produce Persistent Topoisomerase I Cleavage Complexes and Overcome Multidrug Resistance

¹ Laboratory of Molecular Pharmacology and ² Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland and ³ Department of Medicinal Chemistry and Molecular Pharmacology, and the Purdue Cancer Center, Purdue University, West Lafayette, Indiana

Requests for reprints: Yves Pommier, Laboratory of Molecular Pharmacology, National Cancer Institute, 37 Convent Drive, Building 37, Room 5068, NIH, Bethesda, MD 20892-4255. Phone: 301-496-5944; Fax: 301-402-0752; E-mail: pommier{at}nih.gov.

Camptothecin (CPT) derivatives are effective anticancer drugs, especially against solid tumors. As CPTs are chemically unstable and have clinical limitations, we have synthesized indenoisoquinolines as novel topoisomerase I (Top1) inhibitors. We presently report two indenoisoquinoline derivatives, NSC 725776 and NSC 724998, which have been selected for therapeutic development. Both are potent Top1 inhibitors and induce Top1 cleavage at unique genomic positions compared with CPT. Consistent with Top1 poisoning, protein-linked DNA breaks were detected in cells treated with NSC 725776 and NSC 724998 at nanomolar concentrations. Those drug-induced protein-linked DNA breaks persisted longer after drug removal than those produced by CPT. Studies in human cells in culture show that NSC 725776 and NSC 724998 exert antiproliferative activity at submicromolar concentrations. Furthermore, NSC 725776 and NSC 724998 show cross-resistance in cells deficient or silenced for Top1, which is consistent with their selective Top1 targeting. Similar to other known Top1 inhibitors, NSC 725776–treated and NSC 724998–treated cells show an arrest of cell cycle progression in both S and G₂-M and a dependence on functional p53 for their cytotoxicity. Dose-dependent -H2AX foci formation was readily observed in cells treated with NSC 725776 and NSC 724998. These -H2AX foci were detectable at pharmacologically relevant doses for up to 24 h and thus could be used as biomarkers for clinical trials (phase 0). [Cancer Res 2007;67(21):10397–405]

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				Correction: Topoisomerase I Inhibition by NSC 725776 and NSC 724998 Cancer Res., December 15, 2007; 67(24): 12034 - 12034. [Full Text] [PDF]