This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wyckoff, J. B. Articles by Condeelis, J. Search for Related Content PubMed PubMed Citation Articles by Wyckoff, J. B. Articles by Condeelis, J.

Direct Visualization of Macrophage-Assisted Tumor Cell Intravasation in Mammary Tumors

¹ Gruss Lipper Center for Biophotonics and Departments of ² Anatomy and Structural Biology and ³ Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York

Requests for reprints: Jeffrey Wyckoff, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461. Phone: 718-430-3348; Fax: 718-430-8996; E-mail: jwyckoff{at}aecom.yu.edu.

Although the presence of macrophages in tumors has been correlated with poor prognosis, until now there was no direct observation of how macrophages are involved in hematogenous metastasis. In this study, we use multiphoton microscopy to show, for the first time, that tumor cell intravasation occurs in association with perivascular macrophages in mammary tumors. Furthermore, we show that perivascular macrophages of the mammary tumor are associated with tumor cell intravasation in the absence of local angiogenesis. These results show that the interaction between macrophages and tumor cells lying in close proximity defines a microenvironment that is directly involved in the intravasation of cancer cells in mammary tumors. [Cancer Res 2007;67(6):2649–56]

This article has been cited by other articles:

				J. W. Pollard Macrophages define the invasive microenvironment in breast cancer J. Leukoc. Biol., September 1, 2008; 84(3): 623 - 630. [Abstract] [Full Text] [PDF]

				W. Yu, J. Chen, Y. Xiong, F. J. Pixley, X.-M. Dai, Y.-G. Yeung, and E. R. Stanley CSF-1 receptor structure/function in MacCsf1r-/- macrophages: regulation of proliferation, differentiation, and morphology J. Leukoc. Biol., September 1, 2008; 84(3): 852 - 863. [Abstract] [Full Text] [PDF]

				C. Rolny, L. Capparuccia, A. Casazza, M. Mazzone, A. Vallario, A. Cignetti, E. Medico, P. Carmeliet, P. M. Comoglio, and L. Tamagnone The tumor suppressor semaphorin 3B triggers a prometastatic program mediated by interleukin 8 and the tumor microenvironment J. Exp. Med., May 12, 2008; 205(5): 1155 - 1171. [Abstract] [Full Text] [PDF]

				J. Wels, R. N. Kaplan, S. Rafii, and D. Lyden Migratory neighbors and distant invaders: tumor-associated niche cells Genes & Dev., March 1, 2008; 22(5): 559 - 574. [Abstract] [Full Text] [PDF]

				M. Sidani, D. Wessels, G. Mouneimne, M. Ghosh, S. Goswami, C. Sarmiento, W. Wang, S. Kuhl, M. El-Sibai, J. M. Backer, et al. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells J. Cell Biol., November 19, 2007; 179(4): 777 - 791. [Abstract] [Full Text] [PDF]

				C. E. Lewis, M. De Palma, and L. Naldini Tie2-Expressing Monocytes and Tumor Angiogenesis: Regulation by Hypoxia and Angiopoietin-2 Cancer Res., September 15, 2007; 67(18): 8429 - 8432. [Abstract] [Full Text] [PDF]

				W. Wang, J. B. Wyckoff, S. Goswami, Y. Wang, M. Sidani, J. E. Segall, and J. S. Condeelis Coordinated Regulation of Pathways for Enhanced Cell Motility and Chemotaxis Is Conserved in Rat and Mouse Mammary Tumors Cancer Res., April 15, 2007; 67(8): 3505 - 3511. [Abstract] [Full Text] [PDF]