Cancer Research Cancer Epigenetics Sign up for Cancer Research eTOC's
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Cancer Research 67, 4010, May 1, 2007. doi: 10.1158/0008-5472.CAN-06-4180
© 2007 American Association for Cancer Research
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beier, D.
Right arrow Articles by Beier, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beier, D.
Right arrow Articles by Beier, C. P.

Priority Reports

CD133+ and CD133– Glioblastoma-Derived Cancer Stem Cells Show Differential Growth Characteristics and Molecular Profiles

Dagmar Beier1, Peter Hau1, Martin Proescholdt3, Annette Lohmeier1, Jörg Wischhusen5, Peter J. Oefner4, Ludwig Aigner2, Alexander Brawanski3, Ulrich Bogdahn1 and Christoph P. Beier1

1 Laboratory of Neurooncology and 2 VW-Junior Group, Department of Neurology, 3 Department of Neurosurgery, and 4 Institute of Functional Genomics, University of Regensburg, Regensburg, Germany; and 5 Interdisciplinary Center for Clinical Research, Junior Research Group "Tumor Progression and Immune Escape", Clinics for Gynecology and Obstetrics, University of Würzburg, Würzburg, Germany

Requests for reprints: Christoph P. Beier, Department of Neurology, University of Regensburg, Universitätsstrasse 84, 93053 Regensburg, Germany. Phone: 49-941-941-3258; Fax: 49-941-941-3292; E-mail: Christoph.Beier{at}gmx.de.

Although glioblastomas show the same histologic phenotype, biological hallmarks such as growth and differentiation properties vary considerably between individual cases. To investigate whether different subtypes of glioblastomas might originate from different cells of origin, we cultured tumor cells from 22 glioblastomas under medium conditions favoring the growth of neural and cancer stem cells (CSC). Secondary glioblastoma (n = 7)–derived cells did not show any growth in the medium used, suggesting the absence of neural stem cell–like tumor cells. In contrast, 11/15 primary glioblastomas contained a significant CD133+ subpopulation that displayed neurosphere-like, nonadherent growth and asymmetrical cell divisions yielding cells expressing markers characteristic for all three neural lineages. Four of 15 cell lines derived from primary glioblastomas grew adherently in vitro and were driven by CD133– tumor cells that fulfilled stem cell criteria. Both subtypes were similarly tumorigenic in nude mice in vivo. Clinically, CD133– glioblastomas were characterized by a lower proliferation index, whereas glial fibrillary acidic protein staining was similar. GeneArray analysis revealed 117 genes to be differentially expressed by these two subtypes. Together, our data provide first evidence that CD133+ CSC maintain only a subset of primary glioblastomas. The remainder stems from previously unknown CD133– tumor cells with apparent stem cell–like properties but distinct molecular profiles and growth characteristics in vitro and in vivo. [Cancer Res 2007;67(9):4010–5]




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
A. Ernst, S. Hofmann, R. Ahmadi, N. Becker, A. Korshunov, F. Engel, C. Hartmann, J. Felsberg, M. Sabel, H. Peterziel, et al.
Genomic and Expression Profiling of Glioblastoma Stem Cell-Like Spheroid Cultures Identifies Novel Tumor-Relevant Genes Associated with Survival
Clin. Cancer Res., November 1, 2009; 15(21): 6541 - 6550.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
G. Frosina
DNA Repair and Resistance of Gliomas to Chemotherapy and Radiotherapy
Mol. Cancer Res., July 1, 2009; 7(7): 989 - 999.
[Abstract] [Full Text] [PDF]

Home page
 Acta Biochim Biophys SinHome page
W. Zhuang, Z. Qin, and Z. Liang
The role of autophagy in sensitizing malignant glioma cells to radiation therapy
Acta Biochim Biophys Sin, May 1, 2009; 41(5): 341 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
G. Stoica, G. Lungu, H. Martini-Stoica, S. Waghela, J. Levine, and R. Smith
Identification of Cancer Stem Cells in Dog Glioblastoma
Vet. Pathol., May 1, 2009; 46(3): 391 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Wakimoto, S. Kesari, C. J. Farrell, W. T. Curry Jr., C. Zaupa, M. Aghi, T. Kuroda, A. Stemmer-Rachamimov, K. Shah, T.-C. Liu, et al.
Human Glioblastoma-Derived Cancer Stem Cells: Establishment of Invasive Glioma Models and Treatment with Oncolytic Herpes Simplex Virus Vectors
Cancer Res., April 15, 2009; 69(8): 3472 - 3481.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
A. M. McCord, M. Jamal, U. T. Shankavarum, F. F. Lang, K. Camphausen, and P. J. Tofilon
Physiologic Oxygen Concentration Enhances the Stem-Like Properties of CD133+ Human Glioblastoma Cells In vitro
Mol. Cancer Res., April 1, 2009; 7(4): 489 - 497.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J.N. Rich and C.E. Eyler
Cancer Stem Cells in Brain Tumor Biology
Cold Spring Harb Symp Quant Biol, March 27, 2009; (2009) sqb.2008.73.060v1.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
R. Castriconi, A. Daga, A. Dondero, G. Zona, P. L. Poliani, A. Melotti, F. Griffero, D. Marubbi, R. Spaziante, F. Bellora, et al.
NK Cells Recognize and Kill Human Glioblastoma Cells with Stem Cell-Like Properties
J. Immunol., March 15, 2009; 182(6): 3530 - 3539.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Griffero, A. Daga, D. Marubbi, M. C. Capra, A. Melotti, A. Pattarozzi, M. Gatti, A. Bajetto, C. Porcile, F. Barbieri, et al.
Different Response of Human Glioma Tumor-initiating Cells to Epidermal Growth Factor Receptor Kinase Inhibitors
J. Biol. Chem., March 13, 2009; 284(11): 7138 - 7148.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
M. Ropolo, A. Daga, F. Griffero, M. Foresta, G. Casartelli, A. Zunino, A. Poggi, E. Cappelli, G. Zona, R. Spaziante, et al.
Comparative Analysis of DNA Repair in Stem and Nonstem Glioma Cell Cultures
Mol. Cancer Res., March 1, 2009; 7(3): 383 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Genomics ProteomicsHome page
I. L. BOTCHKINA, R. A. ROWEHL, D. E. RIVADENEIRA, M. S. KARPEH JR., H. CRAWFORD, A. DUFOUR, J. JU, Y. WANG, Y. LEYFMAN, and G. I. BOTCHKINA
Phenotypic Subpopulations of Metastatic Colon Cancer Stem Cells: Genomic Analysis
Cancer Genomics Proteomics, January 1, 2009; 6(1): 19 - 29.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. A. Harris, H. Yang, B. E. Low, J. Mukherje, A. Guha, R. T. Bronson, L. D. Shultz, M. A. Israel, and K. Yun
Cancer Stem Cells Are Enriched in the Side Population Cells in a Mouse Model of Glioma
Cancer Res., December 15, 2008; 68(24): 10051 - 10059.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. Pallini, L. Ricci-Vitiani, G. L. Banna, M. Signore, D. Lombardi, M. Todaro, G. Stassi, M. Martini, G. Maira, L. M. Larocca, et al.
Cancer Stem Cell Analysis and Clinical Outcome in Patients with Glioblastoma Multiforme
Clin. Cancer Res., December 15, 2008; 14(24): 8205 - 8212.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
T.J. Pierfelice, K.C. Schreck, C.G. Eberhart, and N. Gaiano
Notch, Neural Stem Cells, and Brain Tumors
Cold Spring Harb Symp Quant Biol, November 19, 2008; (2008) sqb.2008.73.013v2.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J.M Adams, P.N. Kelly, A. Dakic, S. Carotta, S.L. Nutt, and A. Strasser
Role of "Cancer Stem Cells" and Cell Survival in Tumor Development and Maintenance
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.004v1.
[Abstract] [PDF]

Home page
 J Child NeurolHome page
B. A. Emmenegger and R. J. Wechsler-Reya
Stem Cells and the Origin and Propagation of Brain Tumors
J Child Neurol, October 1, 2008; 23(10): 1172 - 1178.
[Abstract] [PDF]

Home page
 Cancer Res.Home page
M. Jaksch, J. Munera, R. Bajpai, A. Terskikh, and R. G. Oshima
Cell Cycle-Dependent Variation of a CD133 Epitope in Human Embryonic Stem Cell, Colon Cancer, and Melanoma Cell Lines
Cancer Res., October 1, 2008; 68(19): 7882 - 7886.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. M. Yi, H.-C. Tsai, S. C. Glockner, S. Lin, J. E. Ohm, H. Easwaran, C. D. James, J. F. Costello, G. Riggins, C. G. Eberhart, et al.
Abnormal DNA Methylation of CD133 in Colorectal and Glioblastoma Tumors
Cancer Res., October 1, 2008; 68(19): 8094 - 8103.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Bao, Q. Wu, Z. Li, S. Sathornsumetee, H. Wang, R. E. McLendon, A. B. Hjelmeland, and J. N. Rich
Targeting Cancer Stem Cells through L1CAM Suppresses Glioma Growth
Cancer Res., August 1, 2008; 68(15): 6043 - 6048.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
P. Y. Wen and S. Kesari
Malignant Gliomas in Adults
N. Engl. J. Med., July 31, 2008; 359(5): 492 - 507.
[Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Beier, S. Rohrl, D. R. Pillai, S. Schwarz, L. A. Kunz-Schughart, P. Leukel, M. Proescholdt, A. Brawanski, U. Bogdahn, A. Trampe-Kieslich, et al.
Temozolomide Preferentially Depletes Cancer Stem Cells in Glioblastoma
Cancer Res., July 15, 2008; 68(14): 5706 - 5715.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
X. Fan and C. G. Eberhart
Medulloblastoma Stem Cells
J. Clin. Oncol., June 10, 2008; 26(17): 2821 - 2827.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
P. B. Dirks
Brain Tumor Stem Cells: Bringing Order to the Chaos of Brain Cancer
J. Clin. Oncol., June 10, 2008; 26(17): 2916 - 2924.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
B. Annabi, S. Rojas-Sutterlin, C. Laflamme, M.-P. Lachambre, Y. Rolland, H. Sartelet, and R. Beliveau
Tumor Environment Dictates Medulloblastoma Cancer Stem Cell Expression and Invasive Phenotype
Mol. Cancer Res., June 1, 2008; 6(6): 907 - 916.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Soeda, A. Inagaki, N. Oka, Y. Ikegame, H. Aoki, S.-i. Yoshimura, S. Nakashima, T. Kunisada, and T. Iwama
Epidermal Growth Factor Plays a Crucial Role in Mitogenic Regulation of Human Brain Tumor Stem Cells
J. Biol. Chem., April 18, 2008; 283(16): 10958 - 10966.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F. B. Furnari, T. Fenton, R. M. Bachoo, A. Mukasa, J. M. Stommel, A. Stegh, W. C. Hahn, K. L. Ligon, D. N. Louis, C. Brennan, et al.
Malignant astrocytic glioma: genetics, biology, and paths to treatment
Genes & Dev., November 1, 2007; 21(21): 2683 - 2710.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2007 by the American Association for Cancer Research.