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ICAM-1 Has a Critical Role in the Regulation of Metastatic Melanoma Tumor Susceptibility to CTL Lysis by Interfering with PI3K/AKT Pathway

¹ Institut National de la Santé et de la Recherche Médicale, U753, Laboratoire d'Immunologie des Tumeurs Humaines: Interaction effecteurs cytotoxiques-système tumoral, Institut Gustave Roussy PR1 and IFR 54; ² Institut Gustave Roussy PR2; Departments of ³ Cytopathology and ⁴ Dermatology, Institut Gustave Roussy, Villejuif, France; ⁵ Chinese Academy of Sciences, Laboratory of Apoptosis and Cancer Biology, The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Beijing, China; and ⁶ Institut National de la Santé et de la Recherche Médicale, U520, Laboratoire de Biologie Cellulaire de l'Immunité Antitumorale, Institut Curie; ⁷ Department of Dermatology, Hôpital Cochin, Paris, France

Requests for reprints: Salem Chouaib, Institut National de la Sante et de la Recherche Medicale U753, Laboratoire d'Immunologie des tumeurs Humaines: Interaction effecteurs cytotoxiques-système tumoral, Institut Gustave Roussy, PR1, 39 rue Camille Desmoulins, F-94805 Villejuif Cedex, France. Phone: 331-42-11-45-47; Fax: 331-42-11-52-88; E-mail: chouaib{at}igr.fr.

Key Words: Human melanoma • metastasis • CTL clone • ICAM-1 • PI3K/AKT pathway

Human primary melanoma cells (T1) were found to be more susceptible to lysis by a Melan-A/MART-1–specific CTL clone (LT12) than their metastatic derivative (G1). We show that this differential susceptibility does not involve antigen presentation by target cells, synapse formation between the metastatic target and CTL clone, or subsequent granzyme B (GrB) polarization. Although PI-9, an inhibitor of GrB, was found to be overexpressed in metastatic G1 cells, knockdown of the PI-9 gene did not result in the attenuation of G1 resistance to CTL-induced killing. Interestingly, we show that whereas T1 cells express high levels of intercellular adhesion molecule-1 (ICAM-1), a dramatically reduced expression was noted on G1 cells. We also showed that sorted ICAM-1⁺ G1 cells were highly sensitive to CTL-induced lysis compared with ICAM-1^– G1 cells. Furthermore, incubation of metastatic G1 cells with IFN- resulted in the induction of ICAM-1 and the potentiation of their susceptibility to lysis by LT12. More importantly, we found that the level of ICAM-1 expression by melanoma cells correlated with decreased PTEN activity. ICAM-1 knockdown in T1 cells resulted in increased phosphorylation of PTEN and the subsequent activation of AKT. We have additionally shown that inhibition of the phosphatidylinositol (3,4,5)-triphosphate kinase (PI3K)/AKT pathway by the specific inhibitor wortmannin induced a significant potentiation of susceptibility of G1 and ICAM-1 small interfering RNA–treated T1 cells to CTL-induced lysis. The present study shows that a shift in ICAM-1 expression, which was associated with an activation of the PI3K/AKT pathway, can be used by metastatic melanoma cells to escape CTL-mediated killing. [Cancer Res 2008;68(23):9854–64]