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Epithelial-Mesenchymal Transition Induced by Growth Suppressor p12^CDK2-AP1 Promotes Tumor Cell Local Invasion but Suppresses Distant Colony Growth

¹ Department of Pathology, Harvard Medical School and ² Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts; ³ Department of Oral and Maxillofacial Surgery, Okayama University Graduate School, Okayama, Japan

Requests for reprints: Guo-fu Hu, Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115. Phone: 617-432-6582; Fax: 617-432-6580; E-mail: guofu_hu{at}hms.harvard.edu.

Key Words: epithelial-mesenchymal transition • Twist2 • invasion • metastasis • p12^CDK2-AP1, E-cadherin

Epithelial-mesenchymal transition (EMT) has been considered essential for metastasis, a multistep process including local invasion, intravasation, extravasation, and proliferation at distant sites. However, controversy remains as to whether EMT truly happens and how important it is to metastasis. We studied the involvement of EMT in individual steps of metastasis and found that p12^CDK2-AP1, a down-stream effector of transforming growth factor β, induced EMT of hamster cheek pouch carcinoma-1 cells by promoting the expression of Twist2. EMT cells have an increased invasive but decreased metastatic phenotype. When s.c. inoculated, both EMT and non-EMT cells established primary tumors, but only EMT cells invaded into the adjacent tissues and blood vessels; however, neither cells formed lung metastases. When i.v. inoculated, only non-EMT cells established lung metastases. Moreover, s.c. inoculation of a mixture of the two cell types resulted in intravasation of both cell types and formation of lung metastasis from non-EMT cells. Our results allowed us to propose a novel model for the role of EMT in cancer metastasis. We showed that EMT and non-EMT cells cooperate to complete the spontaneous metastasis process. We thus hypothesize that EMT cells are responsible for degrading the surrounding matrix to lead the way of invasion and intravasation. Non-EMT cells then enter the blood stream and reestablish colonies in the secondary sites. [Cancer Res 2008;68(24):10377–86]

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				T. Tsuji, S. Ibaragi, and G.-f. Hu Epithelial-Mesenchymal Transition and Cell Cooperativity in Metastasis Cancer Res., September 15, 2009; 69(18): 7135 - 7139. [Abstract] [Full Text] [PDF]