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Cancer Research 69, 5946, July 15, 2009. Published Online First July 7, 2009;
doi: 10.1158/0008-5472.CAN-09-0420
© 2009 American Association for Cancer Research
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Molecular Biology, Pathobiology, and Genetics

Cytogenetic and cDNA Microarray Expression Analysis of MCF10 Human Breast Cancer Progression Cell Lines

Narasimharao V. Marella1, Kishore S. Malyavantham1, Jianmin Wang2, Sei-ichi Matsui3, Ping Liang2 and Ronald Berezney1

1 Department of Biological Sciences, University at Buffalo, State University of New York; 2 Department of Cancer Genetics and 3 SKY Core Resource Facility, Roswell Park Cancer Institute, Buffalo, New York

Requests for reprints: Ronald Berezney, Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260. Phone: 716-645-2363, ext. 154; Fax: 716-645-2975; E-mail: berezney{at}buffalo.edu.

We used a combination of spectral karyotyping, array comparative genomic hybridization, and cDNA microarrays to gain insights into the structural and functional changes of the genome in the MCF10 human breast cancer progression model cell lines. Spectral karyotyping data showed several chromosomal aberrations and array comparative genomic hybridization analysis identified numerous genomic gains and losses that might be involved in the progression toward cancer. Analysis of the expression levels of genes located within these genomic regions revealed a lack of correlation between chromosomal gains and losses and corresponding up-regulation or down-regulation for the majority of the ~1,000 genes analyzed in this study. We conclude that other mechanisms of gene regulation that are not directly related to chromosomal gains and losses play a major role in breast cancer progression. [Cancer Res 2009;69(14):5946–53]




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Correction: Article on Breast Cancer Tumor Progression
Cancer Res., October 1, 2009; 69(19): 7894 - 7894.
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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
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Molecular Cancer Research Cancer Prevention Research
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Copyright © 2009 by the American Association for Cancer Research.