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In vivo Imaging of Cutaneous T-Cell Lymphoma Migration to the Skin

¹ The Wistar Institute, ² Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania; ³ Department of Dermatology, Medical University of Vienna, Vienna, Austria; ⁴ The Centenary Institute for Cancer Medicine and Cell Biology, Newtown, Australia; and ⁵ Discipline of Dermatology, University of Sydney, Camperdown, Australia

Requests for reprints: Wolfgang Weninger, The Centenary Institute for Cancer Medicine and Cell Biology, Locked Bag No. 6, Newtown, New South Wales 2041, Australia. Phone: 61-2-9515-6861; Fax: 61-2-9656-1048; E-mail: w.weninger{at}centenary.org.au.

Key Words: T-cell lymphoma • homing • adhesion molecules • chemokines • microscopy

Cutaneous T-cell lymphoma (CTCL) is characterized by the accumulation of malignant CD4⁺ T cells in the skin. Although the expression of adhesion molecules and chemokine receptors on CTCL cells has been studied extensively on ex vivo isolated cells, very little is known about the dynamics and mechanisms of CTCL trafficking in vivo. However, detailed knowledge of the molecular cues mediating CTCL migration may be used to interfere with their homing to the skin. We made use of real-time intravital epifluorescence video and two-photon microscopy to visualize malignant T cells from Sezary syndrome (SS), a leukemic variant of CTCL, in dermal microvessels in mouse ear skin. We found that SS cells rolled along dermal venules in a P-selectin– and E-selectin–dependent manner at ratios similar to CD4⁺ memory T cells from normal donors. We furthermore show that the chemokine CCL17/TARC, but not CCL27/CTACK, was sufficient to induce the arrest of SS cells in the microvasculature. However, a combination of both chemokines was required to induce extravasation of SS cells. Together, our experiments delineate the molecular adhesion cascade operant in SS cell homing to the skin in vivo. [Cancer Res 2009;69(7):2704–8]